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Citations for PerkinElmer :
451 - 500 of 587 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescence of recombinant pHLuorin2 in each pH calibration buffer was measured using Eppendorf semimicro Vis Cuvettes on an LS 55 Fluorescence spectrometer (PerkinElmer). A xenon lamp was used as the excitation source ...
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bioRxiv - Biochemistry 2020Quote: ... All binding assays were carried out at room temperature in phosphate-buffered saline (PBS) buffer at pH 7.4 and the signals were measured on an VICTOR Multilabel plate reader (Perkin Elmer). Dissociation constants (Kd ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were lysed in 75 µl RIPA lysis buffer containing 0.5% NP-40 and the radioactivity was measured using a Gamma Scintillation Counter (Wallac 1470 (Perkin Elmer), Location) ...
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bioRxiv - Biochemistry 2020Quote: ... Transport was initiated by dilution of 1.5 μl proteoliposomes in 80 μl external buffer (50 mM NaCl and 50 μM or 5 μM [3H]glutamine (PerkinElmer) in 20 mM Tris pH 7.0) ...
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bioRxiv - Neuroscience 2021Quote: ... plates were centrifuged once more under same conditions mentioned above and 5 µL of each FRET antibody pair was added (2.5 nM final concentration for donor and 5 nM for acceptor, diluted in 1x Lance buffer, (Perkin Elmer)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were lysed after 24 hours with Passive Lysis Buffer and the luciferase signal was measured on a Victor multilabel plate reader (PerkinElmer) using the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... cells were plated in a 96-well plate (40,000 cells/well) and were lysed using mammalian lysis cell buffer (Perkin Elmer) and then shaken for 5 min in an orbital shaker at 700 rpm ...
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bioRxiv - Biophysics 2020Quote: Fluorescence emission was recorded in buffer (50 mM Tris-HCl pH 7.4, 50 mM KCl) on a fluorescence spectrometer (LS 55, Perkin Elmer) with excitation/emission slit width of 5 nm and gain values set to 775 V (PMT R928 ...
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bioRxiv - Cancer Biology 2020Quote: ... The filters were washed with 5 ml wash buffer and counted using a liquid scintillation counter (Wallac 1450 MicroBeta Trilux, PerkinElmer). Exchange reactions with GST-Rab35 on glutathione-Sepharose beads were performed in the presence or absence of Arf5 ...
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bioRxiv - Microbiology 2021Quote: ... an equivalent of 500 ng of p24 Gag was lysed in buffer with 1% Triton (ELISA kit, Alliance Perkin Elmer) for 30 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Microbiology 2020Quote: ... Uptake was measured at room temperature by mixing 100 µl of cells with 100 µl of transport buffer containing 8 µM of [14C] glycerol (0.125 µCi each in triplicates, PerkinElmer Inc.). Incorporation of radioactivity was determined as described above.
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bioRxiv - Cell Biology 2023Quote: ... mRFP, mApple, tdTomato) used in (Kurihara et al., 2021) were diluted in PBS buffer and observed using ViewPlate-1536 (PerkinElmer). To observe red fluorescent proteins ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked with TNB blocking buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, and 0.5% TSA blocking reagent [FP102, PerkinElmer, MA, USA]) for 30 min ...
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bioRxiv - Genetics 2024Quote: ... were directly loaded into wells of white strip plates prefilled with 200 µL of Assay Buffer (Perkin Elmer, Waltham, Massachusetts). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... 15 µl of detection buffer (BPS Bio. 33006) containing 7 mg/ml of Glutathione AlphaLisa acceptor beads (Perkin Elmer AL109) and 14 µg/ml of Streptavidin donor beads (Perkin Elmer 676002 ...
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bioRxiv - Neuroscience 2024Quote: ... in an acid medium (KAc/HAc buffer pH 5.2) originates a colorimetric reaction measured at 405 nm in a Victor III (Perkin Elmer) device ...
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bioRxiv - Microbiology 2024Quote: ... RBCs were resuspended in 7 ml parasite culture medium and mixed with 7 ml BrightGlo Lysis Buffer (Perkin Elmer; #E2610). Lysate aliquots were snap frozen in liquid nitrogen and stored at -80° C until use ...
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bioRxiv - Microbiology 2023Quote: ... of the resulting solutions was carried out with ICP-OES (optical emission spectrometry with inductively coupled plasma) Spectra Avio 200 (Perkin Elmer LAS GmbH, Rodgau, Germany) according to DIN standard EN ISO 11885 ...
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bioRxiv - Cell Biology 2023Quote: ... Chemiluminescence was determined in a Fusion® SL chemiluminescence reader (Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) from membranes incubated in Western Lightening® Plus-ECL solution (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Biochemistry 2022Quote: ... 100 μL of the solution was transferred to a black 96-well plate and luminescence read out was performed with a plate reader (PerkinElmer Victor3™ Multilabel Counter 1420).
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bioRxiv - Biochemistry 2022Quote: ... 100 μL of the solution was transferred to a black 96-well plate and the luminescence was measured using a plate reader (PerkinElmer Victor3™ Multilabel Counter 1420).
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bioRxiv - Bioengineering 2022Quote: ... the 96-well sample plate was placed in a designated position on a liquid handling robot (Janus BioTx Pro Plus, PerkinElmer) for automated serial dilution (Fig ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were boiled at 95°C for 10 min and subjected directly to LabChip GX II capillary gel electrophoresis (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the brain hemispheres and two blood samples were weighed in Econo® glass vials for liquid scintillation counting (Perkin Elmer). All samples were treated with a tissue solubilizer (Solvable® ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Diluted samples were analysed by Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) using the NexION 350D ICP-MS (PerkinElmer Inc.) as described previously 134.
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bioRxiv - Plant Biology 2022Quote: ... Radioactivity in each sample was estimated as counts per minute (CPMs) using a Wallac 1450 TriLux MicroBeta 96-well plate scintillation counter (PerkinElmer).
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bioRxiv - Synthetic Biology 2020Quote: ... The sample plate was incubated for 30 min at room temperature in darkness and read on a Victor3 luminometer (PerkinElmer).
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bioRxiv - Genomics 2021Quote: A simultaneous FAME and cholesterol GC-FID method was developed by optimizing two previous methods.92,93 Samples were analyzed on a Perkin Elmer Clarus 500 GC-FID system (Perkin Elmer) equipped with a DB-FFAP polyethylene glycol fused capillary column (30 m × 0.25 mm inner diameter ...
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bioRxiv - Biophysics 2023Quote: ... absorption spectra and fluorescence of degassed and non-degassed samples were recorded using a Lambda 950 spectrophotometer (from Perkin Elmer) and FLS980 fluorometer (from Edinburgh) ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of each sample was used to measure the optical density at 600 nm (OD600) using an EnVision 2105 Multimode Plate Reader (PerkinElmer). 93 μl of each sample were loaded into a black ...
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bioRxiv - Molecular Biology 2022Quote: RNA purified from the IPs and ribodepleted RNA from the input samples were processed for library preparation with the Nextflex Small RNA kit v3 (#NOVA-5132-06, PerkinElmer), following manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... PRO-seq libraries for all replicates were then prepared simultaneously by subjecting one aliquot of 1e7 nuclei/sample to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) following our previously detailed protocol (24) ...
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bioRxiv - Cancer Biology 2024Quote: Images of the drug-treated samples (10-17 images per well) were acquired using Opera Phenix High Content Screening System (PerkinElmer) with 40x objective and Andor Zyla sCMOS camera with Harmony High Content Imaging and Analysis Software (PerkinElmer) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Synthetic Biology 2023Quote: The spectra of three samples (n=3) of both fresh and decellularized banana leaves were collected using a Fourier-transform infrared spectrometer (FTIR) (PerkinElmer Universal ATR ...
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bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer, Austin, Texas, USA). Quality of library preparation was assessed on a 2100 Agilent Bioanalyzer ...
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bioRxiv - Plant Biology 2022Quote: ... and the samples were pipetted in three technical replicates into a 384 well plate using an automated JANUS Workstation (PerkinElmer). According to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were ground to 1mm in particle size and subsequently scanned with a DA 7250 (PerkinElmer Inc., Wellesley, MA, USA) near-infrared reflectance (NIR ...
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bioRxiv - Microbiology 2024Quote: ... Lyophilized biomass samples were analyzed with respect to their carbon content using a PE 2400 Series II CHN elemental analyzer (PerkinElmer). Total fixed carbon in each biomass sample was then determined by multiplying the measured percent carbon by the dry cell weight of the sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The samples were freeze-dried and submitted to a mineralisation procedure using a Titan MPS microwave (Perkin Elmer, Waltham, MA). Each sample was placed into a PTFE digestion vessel ...