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Citations for PerkinElmer :
501 - 550 of 587 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 63%, 40%, 25%) with 9:1 CSBL1:CBL1 buffer (Zeptosens, Bayer) using a Sciclone Caplier ALH3000 liquid handling root (Perkin Elmer). Diluted samples were spotted onto ZeptoChips (Zeptosens ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in buffer A to a final concentration of 4 nM) was added to a 384-well microplate (OptiPlateTM-384 Plus, Perkin Elmer) followed by the addition of 15 μL diluted proteins and 5 μL of the GFP antibody-conjugated acceptor beads (5x) ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Cancer Biology 2020Quote: ... SORE6>GFP sensor expressing MDA-MB-231 primary tumor FFPE sections were treated with 1X citrate pH 6.0 buffer (PerkinElmer, cat# AR6001) and stained with chicken anti-GFP (Novus ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each reaction was diluted in TE and mixed with a PicoGreen working stock (diluted to 1/200× in TE Buffer) to be read with an Enspire plate reader (Perkin Elmer) with 480 nm excitation and 520 nm emission filter ...
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bioRxiv - Biochemistry 2020Quote: ... The 500 nM annealed DNA substrates were then radiolabeled in PNK buffer with PNK enzyme and radioactive ATP [γ32P] from Perkin Elmer at 37°C for 1 hr then diluted in TE buffer (10 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... with 15 µl of sodium phosphate buffer (0.5 M, pH 7.6) was mixed with 37 MBq carrier-free Na125I (NEZ033L010MC, Perkin Elmer, Waltham, US). 4 μl chloramine T (1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA concentration was measured with Quant-iT™ PicoGreen™ dsDNA Assay Kit after it was eluted in 400µl 10mM Tris-EDTA elution buffer (PerkinElmer). Aliquotes of DNA samples were produced with Tecan Genesis/Tecan Freedom Evo and then shipped on dry ice for genetic analyses ...
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bioRxiv - Molecular Biology 2023Quote: ... the SPOT arrays were incubated in methylation buffer containing additionally 50 nM SETD6 and 0.76 µM labelled [methyl-3H]-AdoMet (Perkin Elmer Inc.) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... with 15 μL of sodium phosphate buffer (0.5 M, pH 7.6) was mixed with 37 MBq carrier-free Na125I (NEZ033L010MC, Perkin Elmer, Waltham, US). 4 μl chloramine T (1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the media from each well was transferred to a non-treated 96-well plate and 25ul of AlphaLISA Lysis Buffer (PerkinElmer, #AL001C) was added per well ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... After two washes of 5min in 1X RNAscope wash buffer slides were incubated with 1:1500 Cyanine 3 amplification reagent (PerkinElmer, #FP1170012UG), or 1:1000 Cyanine 5 amplification reagent (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were finally resuspended in the same buffer and aliquoted into three plastic scintillation vials for counting using a MicroBeta2 microplate counter (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
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bioRxiv - Physiology 2023Quote: ... Cells were then lysed in RIPA buffer and 14C content was measured with Gold XR scintillation fluid using a liquid scintillation counter (Perkin Elmer). The glucose uptake was calculated by normalizing counts to protein contents for each sample.
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bioRxiv - Neuroscience 2022Quote: ... 1:200 in TNB buffer or NGS for 2 hours at room temperature and then incubated with HRP-Conjugated Streptavidin (PerkinElmer; USA) 1:100 in TNB buffer or NGS for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times for 5 min in TBS-T buffer and prior to chemiluminescence detection using Western Lightning chemiluminescent substrate (Perkin Elmer). Chemiluminescence signal was detected with an ECL-Imager (INTAS ...
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bioRxiv - Biophysics 2021Quote: ... The sample was subjected to liquid scintillation counting in the Wallac 1450 MicroBeta trilux liquid scintillation and luminescence counter (PerkinElmer, USA). Three or four parallel repeat experiments were performed for the measurement of each data point.
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bioRxiv - Developmental Biology 2021Quote: ... the samples were immunostained as described above after detection of the Emi2 or cyclin B1 RNA probe with tyramide-FITC (PerkinElmer, Inc.) in in situ hybridization analysis ...
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... All treated and RNA-extracted samples were then used to produce small libraries for sequencing using a NETFLEX small RNA-Seq kit (PerkinElmer, USA).
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were diluted into 5% nitric acid and 1% hydrochloric acid and tested on the PerkinElmer NexION 300D (PerkinElmer, Inc.). A calibration curve with known Au concentrations was prepared and the Au concentration was determined according to absorbance values ...
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bioRxiv - Microbiology 2023Quote: ... collected water samples (50 mL) were immediately spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h under in situ natural illumination ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Plant Biology 2023Quote: ... All liquid scintillation samples were counted using a liquid scintillation counter following standard procedures (Tri-Carb 4910TR; Perkin Elmer, Waltham, MA). Radio analysis of 14C-acetate labeled lipid species was completed using an Agilent 1260 Infinity HPLC system coupled to a Lab Logic β-Ram6 for in-line radioactivity detection using modifications of the standardized method (Kotapati & Bates ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were generated from a mixture of poly(A)+ enriched and rRNA-depleted samples using the NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer). Fragmentation of the RNAs was carried out enzymatically at 95°C for 10 min via the addition of NEXTflex® RNA Fragmentation Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 50 μl of media from each sample and 50 μl of the enzyme and substrate mix was pipetted in a well of the imaging plate (PerkinElmer, 6055300). The plate was briefly mixed and incubated for 1 h at RT avoiding exposure to light ...
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bioRxiv - Physiology 2023Quote: ... blood samples were collected and submitted to radiation determination (cpm) in a gamma radiation counter (Wallac Wizard Gamma counter, PerkinElmer, USA). The results obtained were compared with the standard of 99mTc-DTPA and calculated as the percentage product of blood cpm times 100 cpm of administered dose.
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bioRxiv - Bioengineering 2023Quote: UV-vis spectra of the medium and hydrogel samples were measured using a plastic cuvette (1 cm light path) with a UV-visible light spectrophotometer (Lambda 35, Perkin Elmer). The transmittance was measured over the range of 800-400 nm.
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bioRxiv - Molecular Biology 2024Quote: ... the remaining 20% of the beads (supplemented with 40 µl of 2X ROTI®Load and boiled at 95°C for 10 min) were subjected to SDS-PAGE for immunoblotting together with 1% input samples using HRP-streptavidin conjugate (PerkinElmer; #NEL750001EA) or anti-p65 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... and the absorbance of the samples was measured at the wavelength of 370-430nm excitation and emission respectively in Ensight multimode plate reader (Perkin Elmer).
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bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of glycosylated Rac1 was determined by liquid scintillation counting of each sample for 1 min using a Tri-carb 2910TR scintillation counter (Perkin Elmer). The initial rates of the reaction were fit to the Michaelis Menten equation (eq 1 ...
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bioRxiv - Biophysics 2023Quote: ... The uptake reaction was terminated by filtering the samples through a 96 well glass fiber filter (Filtermat B – GF/B, Perkin Elmer) soaked in 1.5% poly(ethyleneimine ...
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bioRxiv - Neuroscience 2022Quote: Fixed samples of Drosophila embryo nerve cords and dissociated mouse commissural neurons were imaged using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon 40X objective with Volocity imaging software ...
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bioRxiv - Plant Biology 2024Quote: ... Zn) in the sample was then analyzed using the ICP-OES instrument (Optima 7300DV, Perkin Elmer Instruments Co., Ltd., Yokohama, Japan). The experiment was repeated twice to test reproducibility.
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence intensity of this protein-free promoter sample was continuously measured for 120 s with a LS-55 fluorescence spectrofluorometer (Perkin Elmer) at 24°C ...
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bioRxiv - Biochemistry 2024Quote: We load equal amounts of 20 µl sample volume for the total protein concentration titration in 384 well plates (PhenoPlate, PerkinElmer, USA). The well plates were chosen due to the superior non adhesive properties that allow to assume spherical shape of the condensates ...
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bioRxiv - Molecular Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubating the section with 0.15% hydrogen peroxide followed by blocking the sections with TNB (blocking) buffer (Perkin Elmer, Woodbridge, Ontario). Sections were incubated with primary antibodies [(c-Kit/CD117 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted at 1/500 in Tris-NaCl blocking buffer (TNB; 100 mM Tris, 150 mM NaCl, 0.5% (w/v) blocking reagent (Perkin Elmer, catalog # FP1012), pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... at 37°C for 1 h in a reaction containing 20 pmol probe, 1X T4 PNK Reaction Buffer, 50 pmol [γ-32P]-ATP (7000 Ci/mmol, 150 mCi/ml)(PerkinElmer, NEG002Z250UC). For hybridization ...
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bioRxiv - Neuroscience 2022Quote: ... diluted at 1/500 in Tris-NaCl blocking buffer (TNB; 100 mM Tris, 150 mM NaCl, 0.5% (w/v) blocking reagent (Perkin Elmer, catalog #FP1012), pH 7.6) ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 µl of radioligand diluted in binding buffer ([3H]-N-methylspiperone: 0.4 nM final concentration, Perkin Elmer, Waltham, MA; [3H]-(R)-(+)-7-OH-DPAT ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μm [γ-32P]GTP (~50 cpm/fmol, Perkin Elmer) at 30 °C ...