Labshake search
Citations for Worthington Biochemical :
1 - 50 of 57 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... 100 µl of nuclease-free water containing 20 units of micrococcal nuclease (MNase, Worthington) was added and enzymatic chromatin digestion was carried out for 25 min at 25°C with 1000 rpm shaking ...
-
bioRxiv - Developmental Biology 2020Quote: ... micrococcal nuclease (Worthington) was added at the concentration of 50 U/ml ...
-
bioRxiv - Biochemistry 2019Quote: ... Microccocal nuclease (MNase; Worthington) was added at a concentration of 10 units/1.0 × 107 cells then incubated at 37°C for 30 min ...
-
bioRxiv - Genomics 2022Quote: ... with micrococcal nuclease (Worthington) for 5 min at 37 °C to recover mono-to tri-nucleosomes ...
-
bioRxiv - Genetics 2019Quote: ... and micrococcal nuclease (Worthington) (60 units for S ...
-
bioRxiv - Genomics 2022Quote: ... with micrococcal nuclease (Worthington) for 5 min at 37 °C to recover mono- to tri-nucleosomes ...
-
bioRxiv - Genomics 2022Quote: ESCs were lysed with micrococcal nuclease (Worthington) in 50 mM Tris pH 7.5 ...
-
bioRxiv - Genomics 2022Quote: ESCs were lysed with micrococcal nuclease (Worthington) in 50 mM Tris pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: Micrococcal nuclease (MNase) was purchased from Worthington Biochemical Corp (Lakewood ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples treated with 0.2 U/µL Micrococcal Nuclease (Worthington) were incubated at 37 °C for 2 h in 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Genomics 2023Quote: ... added 0.5 U of micrococcal nuclease (Worthington Biochemical Corporation) and incubated the tubes for 7 min in a 37°C water bath ...
-
bioRxiv - Genomics 2023Quote: ... nuclei were resuspended in micrococcal nuclease (MNase, Worthington Enzymes) digestion buffer (10 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... High digests (80% mononucleosomes) required 50U of micrococcal nuclease (Worthington, LS004798) and for the low digests ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Systems Biology 2019Quote: ... 50μl of this suspension were digested with micrococcal nuclease (LS004798, Worthington Biochemical Corporation ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... the supernatants were digested with 1 U/mL micrococcal nuclease (Worthington Biochemical) for 40 min at 37 °C ...
-
bioRxiv - Immunology 2022Quote: ... followed by the addition of 75 U of micrococcal nuclease (Worthington Biochemical) and incubated at 37°C for 5 min ...
-
bioRxiv - Genetics 2024Quote: ... Nuclei were aliquoted in tubes with varying concentrations of micrococcal nuclease (Worthington), mixed via tube inversion ...
-
bioRxiv - Genetics 2023Quote: ... Nuclei were aliquoted in tubes with varying concentrations of micrococcal nuclease (Worthington), mixed via tube inversion ...
-
bioRxiv - Genomics 2022Quote: ... 100 ul NP buffer supplemented with 40 units of Micrococcal Nuclease (Worthington MNase, LS004798) were added and samples were incubated for 20 minutes at 37°C ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... 0.1 mM PMSF) were digested with 50 U of micrococcal nuclease (Worthington, Lakewood, NJ) at 30 °C for 2 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... we aliquoted 100 µg of chromatin and added 1 Worthington unit of Micrococcal nuclease (Worthington) per 4.785 µg of chromatin (measured at 260nm ...
-
bioRxiv - Genomics 2022Quote: ... Nuclei from each replicate were first used for a test Micrococcal Nuclease (MNase, Worthington Biochemical) titration ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin was fragmented into ∼90% mononucleosome and ∼10% dinucleosome with Micrococcal Nuclease (MNase) (Worthington Biochem #LS004798). We recommend titrating the MNase digesting condition for each sample ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... 2×106 HCT116 cells were used per each immunoprecipitation (IP) reaction and 15 units of micrococcal nuclease (MNase, Worthington) was used to digest the chromatin for the ChIP reaction ...
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Genomics 2022Quote: ... and pellet was re-suspended in 10ul of lysis buffer supplemented with 17.5 units of micrococcal nuclease (Worthington MNase, LS004798) and chilled on ice for 10 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Developmental Biology 2019Quote: ... Cells then were resuspended in 100 μl 1X PBS with CaCl2 (final concentration: 1mM) and TritonX100 (final concentration: 0.1%) and incubated with 5 Units micrococcal nuclease (Worthington Biochemical, LS004798) at 37°C for 3 min ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Molecular Biology 2023Quote: Uterine pieces were washed with phenol red-free free HBSS(−) to remove epithelial cells and further incubated in phenol red-free free HBSS(−) with collagenase type III (#CLS3, Worthington, Lakewood, NJ) at 37.5°C for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... 1% (w/v) fatty acid-free BSA (Worthington, USA) was prepared by dissolving fatty acid-free BSA in serum-free DMEM containing 4 μM L-glutamine ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Physiology 2024Quote: ... The Ca2+ free buffer was then supplemented with 0.66mg/mL collagenase (Worthington; Type II), 0.033mg/mL trypsin and 0.05mM CaCl2 and perfusion continued for a further 10 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... mechanically disrupted and digested in serum free media containing 2mg/ml collagenase type I (Worthington) and DNase I (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Worthington Biochemical) at 37°C for 16 hours ...
-
bioRxiv - Physiology 2022Quote: ... comprised of Ca2+-free solution with the addition of 200 U/mL Collagenase II (Worthington Biochemical Corporation), 0.06 mg/mL Protease XIV (from Streptomyces griseus ...
-
bioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1 ...
-
bioRxiv - Bioengineering 2022Quote: ... vessels were removed from the perfused system and incubated in 3 Kunitz/ml protease-free DNAse (Worthington, LS006343) in 1x enzyme reaction buffer (40 mM Tris-HCl pH 8.0 ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. ...Cat# LS006343, 50 ku, $640.00 Ask -
bioRxiv - Biochemistry 2020Quote: ... DRGs were digested in 1 mL of Ca2+/Mg2+-free HBSS containing 20 U of papain (Worthington, Lakewood, NJ), 0.35 mg of L-cysteine and 1 μL of saturated NaHCO3 and incubated at 37°C for 10 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... All four cell lines were infected with SARS-CoV-2/Australia/VIC01/2020 at a multiplicity of infection of 0.1 with infection inoculum composed of serum-free culture media and TPCK-treated trypsin (Worthington). The plates were incubated at 37 °C for 30 minutes ...
-
bioRxiv - Biophysics 2019Quote: ... cleaned and cut into pieces in ice-cold calcium- and magnesium-free Dulbecco’s phosphate buffered saline and digested for 30 min in papain (10 units/ml; Worthington) at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... 3 mL of overlay (1.2% Avicel in FBS-free DMEM supplemented with 0.1 mg/mL DEAE dextran and 1 μg/mL Worthington’s trypsin) was added to each well and plates were incubated for 3-4 days ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
-
bioRxiv - Physiology 2022Quote: ... before perfusion was switched to EGTA-free isolation buffer supplemented with 1 mg/mL collagenase (type II; Worthington Biochemical, USA), 0.06 mg/mL protease XIV ...
-
bioRxiv - Microbiology 2020Quote: ... 1 hour after infection media was changed to serum-free media supplemented with 1µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington-Biochemical). Passage experiments were performed by infecting cells at an MOI of 0.01 ...
-
bioRxiv - Microbiology 2019Quote: ... was propagated in MA104 cells in serum-free DMEM in the presence of 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). After harvest ...
-
bioRxiv - Cancer Biology 2023Quote: Xenograft tumors were harvested and digested using 5mL digestion media (phenol red-free DMEM/F12 [Corning cat. 16-405-CV], 100 mg/mL gentamicin [Worthington, cat ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
-
bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask