Labshake search
Citations for Worthington Biochemical :
1 - 50 of 523 citations for Mouse Anti Dengue Virus Envelope Protein Serotypes 1 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
-
bioRxiv - Microbiology 2019Quote: ... tissue supernatants were serially diluted 10-fold in virus infection medium supplemented with 1 µg/mL L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ). The plates were rinsed twice with PBS ...
-
bioRxiv - Bioengineering 2020Quote: ... Infection with the H1N1 virus was performed in the presence of 1 µg of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin/ml (Worthington Biomedical).27 Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... and then treated with 1 mg/ml collagenase type 3 (Worthington Biochemical Corporation ...
-
bioRxiv - Cell Biology 2024Quote: ... After enzymatic digestion (DMEM GlutaMAX, 1% P/S, 3% papain [Worthington 3126] ...
-
bioRxiv - Microbiology 2023Quote: ... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
bioRxiv - Developmental Biology 2022Quote: ... the mouse knee was minced into small fragments and digested with 3 mg/ml collagenase type I (Worthington) and 4 mg/ml dispase (Roche ...
-
bioRxiv - Neuroscience 2020Quote: ... incubated with 3% collagenase type 3 (Worthington), 3 U/ml dispase (Worthington ...
-
bioRxiv - Neuroscience 2023Quote: ... 165859) and collagenase type II (3 mg mL −1, cat. no. 4176, Worthington) for ∼40 min at 37 ⁰C under gentle agitation and centrifuged at 800 x g for 7 minutes ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Tibiae and femora from each mouse were combined and incubated with 4 ml of collagenase solution (3 mg/ml Collagenase Type IV in PBS, Worthington Biochemical Corp., Lakewood, NJ) inside a 5% CO2 chamber at 37°C for 15 min under continuous agitation ...
-
bioRxiv - Microbiology 2021Quote: ... At pre-determined timepoints post-infection 100μL of PBS (or in the case of influenza virus PBS + 0.1 μg of TPCK-treated trypsin (Worthington, USA)) was added to the apical compartment and cells were incubated at 37°C with 5% CO2 for 10 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... After tissue digestion by 75 U/ml collagenase type 3 (Worthington #CLS-3, S8P18814) for 15 min ...
-
bioRxiv - Neuroscience 2020Quote: ... 3 U/ml dispase (Worthington) and DNase (Millipore Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... DNase I (vial 3, Worthington), Ovomucoid protease inhibitor (vial 4 ...
-
bioRxiv - Systems Biology 2024Quote: ... 3 U/mL Elastase (Worthington), and 20 U/mL DNAse (Worthington ...
-
bioRxiv - Systems Biology 2024Quote: ... 3 U/ml Elastase (Worthington), and DNAse I (Worthington) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Type 3 (Worthington Biochemical LS004216), and Type 4 (Worthington Biochemical LS004212) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Biochemistry 2020Quote: Reverse transcription reactions with Moloney Murine Leukemia Virus (MMLV) RT (BioBharati Life Sciences Pvt. Ltd) and HIV RT (Worthington Biochemical) were carried out according to the manufacturer’s directions using primer P5 (Table S1) ...
-
bioRxiv - Cell Biology 2019Quote: ... each consisting of calvaria from 6 pups were digested in 10 ml collagenase solution (1 mg/ml Collagenase type I and type II (1:3 ratio, Worthington Biochemical Corp., Lakewood, NJ), 1 mM CaCl2 ...
-
bioRxiv - Cancer Biology 2024Quote: Mouse tumors were digested in a 1 mg/mL collagenase IV and 0.1 mg/mL DNSase 1 (Worthington Biochemical, Lakewood, NJ) in HBSS for 45 minutes at 37 °C with intermittent shaking ...
-
bioRxiv - Developmental Biology 2022Quote: ... MM from E11.5 kidneys from CD-1 mice were dissociated with Collagenase Type 3 (Worthington Biochemical Corporation; Cat #LS004180) and DNase I (New England Biolabs ...
-
bioRxiv - Developmental Biology 2022Quote: ... and digested with gentle rocking for 1 h at 37°C in 3 mg/mL collagenase II (Worthington, LS004176) diluted in HBSS containing 5% fetal bovine serum (FBS) ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... The minced tumors were digested with 5ml to 10ml of 1 mg/ml Collagenase 3 (Worthington cat. no. LS004183) plus 0.5mg/ml Hyaluronidase (Sigma-Aldrich H3884 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Biochemistry 2019Quote: ... Cortex tissues obtained from mouse brains on postnatal day 1 were dissociated with papain (Worthington Biochemical). Dissociated cells (2.0 × 104 cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... or a cocktail for mouse lung digestion composed of Collagenase Type I (CLS-1 LS004197, Worthington), Elastase (ESL LS002294 ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Immunology 2021Quote: Back skin and ears were cut into smaller pieces and incubated in collagenase 3 (3 mg/ml, Worthington), DNAse I (5 µg/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... and 3 U/mL dispase (LS02104, Worthington). After 37□°C incubation for 45□min ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and adenosine deaminase (3 U/ml, Worthington) for 30 min followed by addition of agonists or vehicle control and incubated for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... The RT units of a concentrated stock of HIV Q23.BG505 virus were determined multiple times using a standard curve of purified HIV RT enzyme (Worthington Biochemical Corp. #LS05003). Aliquots of this titered stock of Q23.BG505 were used as the quantitative standard curve in all assays ...
Product cited:
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10 mM...Cat# LS05003, 200 un, $95.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... and the telencephalon was manually cut into pieces, followed by enzymatic digestion (DMEM GlutaMAX 1% P/S, 3% papain (Worthington 3126), 1.5% 10 mg ml−1 DNAse and 2.4% 12 mg ml−1 cystein ...
-
bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
-
bioRxiv - Developmental Biology 2024Quote: ... Zebrafish ventricles were fixed in 3% PFA for 5 min and incubated in PBS supplemented with 1 mg/mL collagenase type 4 (Worthington Biochemical) at 4°C overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) digestion buffer (at ratio of 8μL / mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The tissue pieces from each sample were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) in Hibernate-E medium (at ratio of 8μL/mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... 150 U/mL Collagenase type 3 (Worthington, LS004183), 20 µg/mL Liberase Blendzyme 2 (Roche ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Pathology 2023Quote: ... digested with collagenase (3 mg/mL, Worthington, S3N6800)/Dispase (1 mg/mL ...
-
bioRxiv - Biophysics 2024Quote: ... and 0.83 mg/ml collagenase type 3 (Worthington Biochemical Corporation ...
-
bioRxiv - Genomics 2024Quote: ... 3 mg/mL collagenase II (Worthington, cat. LS004174), and 1.6 mg/mL hyaluronidase (Worthington ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
-
bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
-
bioRxiv - Genomics 2022Quote: ... Bulk mammary tissues were mechanically processed into a slurry and digested overnight with collagenase type 3 (200U/mL, Worthington Biochem CLS-3) and hyaluronidase (100U/mL ...
-
bioRxiv - Neuroscience 2020Quote: ... The remaining frozen tissue was weighed and briefly sliced on dry ice and then incubated in 75 U/ml collagenase type 3 (Worthington #CLS-3, S8P18814) in Hibernate-E solution (human tissue for 20 min ...
-
bioRxiv - Cell Biology 2023Quote: ... limb buds from the E12.5 mouse embryo were isolated and digested with 1 mg/ml type II collagenase (Worthington, LS004176) for 1 h at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask