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Citations for Worthington Biochemical :
301 - 350 of 370 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from HCT116 wild-type or knockout cells and treated with DNase I (Worthington Biochemical) at 25 °C for 20 min ...
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bioRxiv - Genomics 2020Quote: ... Cortices acquired from multiple sets of male littermates were incubated in activated 20 U/mL Papain/DNase (Worthington) for 15 minutes at 37°C ...
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bioRxiv - Genomics 2021Quote: ... 1 mL of lung dissociation medium (100 µg/mL of DNAse I, Roche; 1.25 mg/mL of Pronase, Roche; 9.2 µg/mL of Elastase, Worthington; 100 µg/mL of Dispase II ...
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bioRxiv - Developmental Biology 2022Quote: ... and placed in a prewarmed digestion enzyme mix - inflation enzyme mix plus 0.5mg/mL DNase 1 (Worthington Biochemical). They were placed in an incubator at 37C and rotated for 15min ...
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bioRxiv - Neuroscience 2023Quote: ... Organoids were dissociated into single cells using a 1:20 mixture of DNAse in PBS (Worthington Biochemical, LK003163) and TrypLE (Thermo Fisher ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... hCOs were dissociated into single cells using a 1:20 mixture of DNAse in PBS (Worthington Biochemical, LK003163) and TrypLE (Thermo Fisher ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... lung was digested with a mixture of Collagenase IV (1.6 mg/ml) and DNase (50 unit/ml) (Worthington) at 37°C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... isolated cerebellar were digested with trypsin (10 mg/ml)-DNase (200 units/ml) (TRL, DP, Worthington, Lakewood, NJ) solution ...
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bioRxiv - Neuroscience 2024Quote: ... The mechanical dissociation was performed in ice-cold DMEM/F12- containing 40 units/mL DNAse (cat#: LK003172; Worthington) and adapted according to Mattei et al ...
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bioRxiv - Cancer Biology 2024Quote: ... dissociated mechanically and enzymatically (1 mg/mL type-IV collagenase and 0.25 mg/mL DNAse I, Worthington Biochemical) and fractionated in a Percoll gradient (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... Media was aspirated and cells were resuspended in DMEM/F12 supplemented with 100ug/mL DNase (Worthington Biochemical, LS002006). After incubating in DNase ...
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bioRxiv - Genetics 2020Quote: ... the cells were resuspended in EBSS containing 50μg/mL of DNase I and 2 mg/mL ovomucoid (Worthington, LK003150). 0.5 volume of EBSS ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... The cells were washed with the buffer and were incubated with 0.2 mg/mL DNase I (Worthington Biochemical, US) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... mechanically dissociated and transferred to basal medium containing 0.1% collagenase Type IV and 150 mg/ml DNase I (Worthington), and incubated for 30 min shaking at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Fresh retinae were dissected before being placed in activated papain/DNase (1:20 solution; Worthington Biochemicals, New Jersey, USA) and incubated at 37°C for 45 minutes on a shaker ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in Dispase II (5 mg/mL; StemCell 7913) and DNase I (100 μg/mL; Worthington Biochemical LS002006) for 5 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... A secondary digestion was performed to liberate additional cells from tissue by transferring approximately 8 ml of tissue and collagenase solution to a 10 ml Falcon tube containing 0.2 mg DNase (LS002006, Worthington) in 500 μl BSA ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... Micro-dissected regions were dissociated by incubating with papain (20 units/ml) DNase (0.005%) for 30min at 37°C (Worthington). Samples were triturated in HEPES buffered EBSS to fully dissociate the tissue and centrifuged (3 min ...
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bioRxiv - Neuroscience 2020Quote: ... and lysed with freeze/thawing in hypotonic buffer containing Dulbecco’s Modified Eagle Medium (DMEM, Welgene) and deoxyribonuclease I (DNase I, Worthington). After removing cell debris through centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated during 10 min at RT with 0.1 mg/ml of DNAse I (PN LS002007, Worthington-Biochem) in order to eliminate ambient DNA and favor the resuspension of the pellet ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... The collected tissue was incubated with continuous shaking at 37°C for 45 min in 1 ml of papain solution (20 U/ml) and 0.2 ml DNase (both from Worthington) prepared in isolation buffer ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001], 0.5 mg/ml DNAse I [Worthington Biochemical ...
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bioRxiv - Immunology 2023Quote: ... The decidua was mechanically dissociated and digested in RPMI-1640 supplemented with 40 U ml-1 DNAse I (Worthington) and 300 U ml-1 collagenase I (Worthington ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg/ml Dispase II (MilliporeSigma, catalog #D4693) and 50 U/ml of DNase I (Worthington Biochemical, catalog #LS002006). Cells were washed in ice-cold PBS with 3% FCS and 2 mM EDTA (FACS buffer ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Immunology 2023Quote: ... one lobe (1mg/ml Collagenase II, 2.5mg/ml Collagenase IV Worthington LS004188 and 15ug/ml DNAse I, 50 min). Kidney ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... dispase (0.2 U/ml) and DNAse (168 U/ml) dissolved in Earle’s Balanced Salt Solution (EBSS, Worthington Biochemical Corporation). Up to 6 retinae were put in 1.7 ml of dissociation solution ...
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 40-60 wing discs were dissociated in 250 μL of Papain (20 U/mL) and DNase I (200 U/mL) in Earle’s Buffered Salt Solution (all from Worthington Biochemical), at 25°C for 40 minutes ...
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bioRxiv - Developmental Biology 2020Quote: Prostate tissues were minced with scissors and then incubated in papain (20 units/ml) with 0.1 mg/ml DNase I (Worthington LK003150) at 37°C with gentle agitation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... followed by quenching of the enzyme using 1 mg/ml ovomucoid/bovine serum albumin solution with 0.1 mg/ml DNase I (Worthington LK003150). Cells were passed through a 70 μm strainer (PluriSelect 43-10070-70 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Genetics 2020Quote: ... supplemented with 10 μM Rock inhibitor and 33 μg/mL DNase I (Worthington, LK003170, only for days 30 and 52). The cells were dissociated using a P1000 and collected in a 15 mL tube capped with a 40 μm cell strainer ...
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Bioengineering 2022Quote: ... The distal lung tissue was separated from the mainstem bronchi and transferred to a Petri dish containing DMEM with 1% HEPES and 100 U/mL DNase I (Worthington). The tissue was then dissociated with a blunt bone marrow aspiration needle ...
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bioRxiv - Immunology 2020Quote: Freshly resected tumor samples were mechanically dissociated and digested in HBSS medium with Collagenase IV (2.5 mg/mL) and DNAse I (0.2 mg/mL) (Worthington Biochemical Corporation) at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: Hearts from E14.5 embryos were isolated and incubated 30 minutes at 37°C on a rotating shaker with 200 µl of digestion solution per embryo consisting out of 0.05 mg/ml DNase I (Worthington, 2139) and 1 mg/ml Collagenase I (Worthington ...
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bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... Cortical organoids were dissociated into single-cell suspensions via 30-min incubation at 37 °C in papain and DNAse in EBSS (Cat. # LK003150, Worthington), shaking vigorously every 5-10 min ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... samples were coarsely chopped with surgical scissors and enzymatically digested with Papain supplemented with DNase I (Worthington Biochemical Corporation, LK003150). Samples were incubated for 15 minutes at 37°C on a thermocycler kept at 1400 g and briefly pipetted every 5 minutes ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Genetics 2022Quote: ... cortices and hippocampi acquired from 12 male mice from 3 separate litters were combined and dissociated using activated 20 U/mL Papain/DNase (Worthington) diluted in Hibernate A (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Cancer Biology 2024Quote: Tumor-transplanted organoids were transferred to a 1.7 mL Eppendorf tube containing 1 mL Papain supplemented with 50 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Systems Biology 2024Quote: ... WT and Elavl2 knockout EBs were collected into Eppendorf tubes where the medium was replaced with papain solution containing 20 U/mL papain and 67 U/mL DNase (Worthington). Tubes were incubated for 45-60 minutes at 37° C under constant agitation ...
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bioRxiv - Neuroscience 2024Quote: ... the retinas were incubated for 7 min at 37°C in a solution of papain and deoxyribonuclease (DNase) (Worthington, #LK003172). Incubation was followed by trituration of the mixture to dissociate the cells ...
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Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3XbioRxiv - Neuroscience 2024Quote: ... Cortices from single embryos were isolated in ice-cold Hank’s balanced salt solution (HBSS) and then digested with pre-warmed papain containing DNase (Worthington, #LK003150) at 37°C and 5% CO2 for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Worthington Biochemical) at 37°C for 16 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Cell mixture is centrifuged 6 minutes at 300 x g and after carefully aspirating the supernatant the cell pellet is then resuspended with EBSS plus ovomucoid inhibitor albumin mixture supplemented with DNase (each part of Worthington kit). Gentle trituration with a 10 ml pipette held several centimeters from the tube bottom is sufficient to resuspend ...
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bioRxiv - Cell Biology 2020Quote: ... finely minced endometrial tissue was enzymatically and mechanically digested in Dulbecco’s modified Eagle’s medium (DMEM/F12) supplemented with collagenase type I and DNase I (Worthington Biochemical Corporation, USA) at 37°C on a rotating MACSmix (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2023Quote: ... 3-4 pooled organoids at 3-4 months of age or three single organoids at 2-month age from both haploinsufficient and corrected lines were transferred to a 60 mm dish containing a papain and DNase solution from the Papain Dissociation Kit (Worthington, #LK003150). Organoids were minced into small bits with razors and incubated for 30 min at 37°C on an orbital shaker ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... with 2 mL pre-warmed digestion enzyme in ACSF solution (Papain, 25 unit/mL and DNase I, 55 unit/mL, Worthington Biochemical). For spinal dorsal horn neuronal atlas construction ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... the implants were enzymatically digested with the digestion solution (200 U/ml Collagenase IV and 125U/ml DNase I, both from Worthington Biochemical) in RPMI media (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenate was digested for 15 minutes in enzymatic solution (0.2 µm filter sterilised 4.5 U/mL papain [Worthington Biochemicals], 1× DNAse [Worthington Biochemicals], in PBS) at 37 °C ...