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Citations for Qiagen :
601 - 650 of 5763 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... QuantiTect Primer Assays (Qiagen) or primers designed with the Primer3 software (http://primer3.ut.ee ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: QuantiTect Primer Assays (Qiagen) were used for all the genes analysed ...
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bioRxiv - Physiology 2022Quote: ... Primers designed by Qiagen for LKB1 were not used as they detect area of the genes that are not within the floxed loxP sites ...
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bioRxiv - Physiology 2022Quote: ... primers obtained from Qiagen were used to detect the expression of tyrosine hydroxylase (QuantiTect Primer Assay ...
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bioRxiv - Neuroscience 2019Quote: ... Gad2 promoter primers (Qiagen) and Methylated DNA and unmethylated DNA control primers (Diagenode ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... 0.4 µM primers (Qiagen), and cDNA to a total input volume of 10 µL ...
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bioRxiv - Immunology 2023Quote: ... QuantiTect Primer Assay (Qiagen) against 18S ribosomal RNA (QT00199367) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitect primers from Qiagen: Hs_TFEB_1_SG QuantiTect Primer Assay (QT00069951) ...
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bioRxiv - Genomics 2020Quote: Bisulfite converted DNA was amplified with a region-specific biotinylated/unmodified DNA primer pair (Metabion; Supplemental Table S4) using the PyroMark PCR Kit (Qiagen) according to the manufactures instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Relative gene expression was measured using RT2 Profiler low-density PCR plates preloaded with qPCR primer assays for genes encoding GABA- and glutamate-related targets (PN: PARN-152ZA, Qiagen). Thermal cycling and data collection were accomplished using an ABI Real-Time PCR 7300.
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bioRxiv - Bioengineering 2020Quote: ... The prepared cDNA was preamplified using the RT2 PreAMP Primer Mix for Human and Mouse PCR Array (Qiagen, PBH-181Z). cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was then amplified using qPCRBio SyGreen Mix (PCR Biosystems, Wayne, PA, USA) and QuantiTect Primer Assays (Qiagen, Hilden, Germany) for ACE2 (Hs_ACE2_1_SG ...
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bioRxiv - Immunology 2021Quote: ... The IgGH gene lacking the leader sequence was amplified by PCR with the given primers and purified using the MinElute Reaction cleanup kit (Qiagen), followed by digestion with BamHI-Hf and NotI restriction enzymes (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA probes were PCR amplified using specific primers and gel purified using a Qiagen MinElute Gel Extraction Kit (Qiagen). EMSAs were performed by adding increasing amounts of purified OrhR-His6 fusion protein to a DNA probe (40 ng ...
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bioRxiv - Pathology 2022Quote: ... The N gene-specific primers were used to amplify 97 bp of SARS-CoV-2 N gene by conventional PCR and purified by Qiagen gel extraction kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... were amplified using biotin-labeled primers (Supplementary Table 2) synthesized by Sangon Biotech (Shanghai, China) and purified by a PCR purification kit (Qiagen). The EMSA was conducted as described previously(An et al. ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions contained 4 μl of cDNA (1:50 dilution) and 200 nM of each primer (supplementary table 1) and QuantiTect SYBR Green PCR master mix (Qiagen) or SensiFAST SYBR Hi-ROX kit (Bioline) ...
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bioRxiv - Microbiology 2022Quote: ... The synthetic sglPP7 DNA was amplified using primers KC94 and KC116 and the resulting PCR product was gel purified (Qiagen), digested with restriction enzymes EcoRI and XhoI (New England Biolabs) ...
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bioRxiv - Biochemistry 2019Quote: ... The bisulfite treated DNA was then used for PCR amplification using the amplicon and primers with treatment specific barcodes and HotStarTaq DNA Polymerase (Qiagen). PCR products were resolved on an 1% agarose gel ...
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bioRxiv - Developmental Biology 2020Quote: ... For allelic-skewing analysis the cDNA was PCR-amplified with biotinylated primers and sequenced using the Pyromark Q24 system (QIAGEN).
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (QPCR) using gene-specific primers (supplementary table S9) was performed using QuantiNova SYBR Green mix (Qiagen) on an MX3000P real-time PCR machine (Stratagene) ...
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bioRxiv - Microbiology 2019Quote: ... the coding region of MW1875 or MW1874 amplified by PCR using specific primers was cloned into pQE30 (QIAGEN, Tokyo, Japan) which is used for the construction of histidine-tagged recombinant proteins ...
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bioRxiv - Neuroscience 2019Quote: ... PCR reactions were performed using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen: mmu-miR-598-3p ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Viral DNA levels were quantified by qPCR using primers and probe that detect the ASFV VP72 gene52 with the Quantifast Pathogen PCR kit (Qiagen) and the following cycle conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.2 uM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, ß-actin) and 12.5 ul of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
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bioRxiv - Developmental Biology 2020Quote: ... (underlined region is the T7 promoter for In vitro transcription) These primers amplified exonic region of Dyrk1b and the product was cleaned using PCR purification kit (Qiagen)and cloned into pGEM®-T Easy Vector Systems (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... SiCBL4 and SiCBL7 promoters were respectively amplified using biotin-labeled primers (Table S1) synthesized by Sangon Biotech (Shanghai China) and purified using a PCR purification kit (Qiagen). EMSA was conducted using a LightShift Chemilumniescent EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Integration of the repair construct into the genome was verified by diagnostic PCR using primers 40-43 on parasite genomic DNA extracted using the DNeasy Blood and Tissue Kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... The primers were used in a 28 cycle PCR with the HotStarTaq Plus Master Mix Kit (Qiagen Inc., Hilden, Germany). The PCR cycle conditions were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Actin was used as a housekeeping gene to normalize the amount of viral RNA by using the qPCRBIO SyGreen mix HI-ROX (PCR Biosystems) together with QuantiTect primer assay (QT01680476, Qiagen). All Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2019Quote: ... and qPCR was performed using gene specific QuantiTect Primer Assay primers from Qiagen. Relative expression levels were normalized to gapdh expression according to the formula <2^− (Ct gene of interest − Ct gapdh ...
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bioRxiv - Cancer Biology 2020Quote: ... and internal control EIF3D and RPL13A specific primers (RT2 qPCR Primer Assays -Qiagen) and RT² SYBR® Green qPCR master mix using the recommended protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Primers used against DDIT3 (CHOP) were commercially available QuantiTect primer assays (Qiagen, UK). Please refer to table S2 and S3 in the supplementary material for the sequences of other primers used ...
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bioRxiv - Plant Biology 2020Quote: The real-time quantification of cDNA corresponding to 2µg of total RNA was performed in the ABI PRISM 7900HT sequence detection system using the QuantiTech SYBR Green RT-PCR kit (Qiagen). The Gene-specific primers used are listed in Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay12 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted by quantitative RT-PCR from Col-0 and DWF4-OE seedlings 6 days post germination with the RNeasy Mini Kit (Qiagen). For DWF-OE ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR assay (24, 25) using the QuantiFast probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Genetics 2022Quote: SARS-CoV-2 RNA copy number was quantified in RNA samples extracted from infected cells using the QuantiTect probe RT-PCR Kit (Qiagen). TWe prepared a standard curve using the ORF9 nucleoprotein coding sequence cloned in pcDNA3 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then diluted 25 times to serve as template for real-time quantitative PCR (RT-qPCR) using Rotor-Gene 6000 (Corbett Research, QIAgen), as previously described (Forlenza et al. ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were done with the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
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bioRxiv - Microbiology 2020Quote: The 16 h clindamycin-induced and uninduced seed cultures were cultivated and incubated in 1 ml of RNA Protect in the same manner as for RT-PCR Total RNA was extracted with an RNeasy RNA isolation kit (Qiagen). The isolated RNA was treated with DNaseI (0,1 U μl-1 ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR assay (Corman et al., 2020; Wolfel et al., 2020) using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The SVIP-MPv2 assay was developed and validated as a one-step procedure using the QuantiNova Probe RT-PCR Kit (Qiagen) with final concentrations of primers (1.4μM each ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA (5 μl) was used for cDNA synthesis and qPCR was performed in one step using QuantiTect Probe RT-PCR (Qiagen) on a StepOnePlus System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Gene expression was evaluated by qPCR using the ONE-step QuantiTect Probe RT-PCR Kit (Qiagen, Cat No./ID: 204445). For each reaction 2 μL of RNA were used ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10ng of RNA per sample was used in each reaction according to instruction in QuantiFast SYBR Green RT-PCR kit (QIAGEN). Samples were subsequently loaded on to a LightCycler 480 Instrument II (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay33 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Pathology 2021Quote: ... and gene expression was carried using the RT² Profiler PCR Array for human cellular stress responses (Qiagen, Catalog#:PAHS-019ZA) following manufacturer’s instructions ...