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Citations for Qiagen :
501 - 550 of 5763 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and RNase-Free DNase Set (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNase-free DNase Set (Qiagen) was used for on-column DNase digestion ...
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bioRxiv - Genetics 2019Quote: ... and RNase-Free DNase Set (QIAGEN). Adaptors as well as low quality base pairs were trimmed ...
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bioRxiv - Microbiology 2021Quote: ... The RNase-Free DNase Set (Qiagen) treatment was applied to all samples according to the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2023Quote: ... and RNase-Free DNase Set (QIAGEN) according to the product instructions ...
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bioRxiv - Immunology 2023Quote: ... with Rnase-Free DNase set (Qiagen). The RNA was eluted with RNAse-free H2O in final volume of 20μl and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... and RNase-Free DNAse Set (Qiagen). Total RNA (150 ng ...
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bioRxiv - Immunology 2023Quote: ... including RNase-Free DNase Set (Qiagen) treatment ...
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bioRxiv - Immunology 2023Quote: ... and RNase-Free DNase Set (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... including RNase-Free DNase Set (Qiagen) treatment ...
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bioRxiv - Microbiology 2023Quote: ... and RNase-free DNase Set (Qiagen) on-column DNA digestion ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and RNase-Free DNase Set (QIAGEN) according to product instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase-free DNase Set (Qiagen). Infection was confirmed by RT-qPCR using specific primers to detect HDV transcripts.
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Cell Biology 2021Quote: ... Validated qPCR primers and primer assays (Qiagen, see Table 1) were used together with QuantiTect SYBR Green PCR kit (QIAGEN ...
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bioRxiv - Genetics 2022Quote: ... Routine qPCR for miR detection and validation (see Supplementary Table 1 for miR sequences) was performed with the miScript PCR control set (catalog number 218380; Qiagen, Germany). The miScript™ miRNA PCR Array Human Serum & Plasma 384HC (Cat No ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.0–3.0 μl (5.0–15 pmol) of primers and 7.5–15 μl of 2x Multiplex PCR Plus Master mix (QIAGEN). The PCR protocol consisted of an initial DNA polymerase (HotStar Taq ...
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bioRxiv - Cancer Biology 2020Quote: ... 1μl of forward/reverse primers (5pmol) and 3μl of nuclease-free water were mixed in a 0.1mL real-time PCR (Qiagen) to conduct PCR in a 10μl of reaction volume ...
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bioRxiv - Genomics 2022Quote: ... and then PCR-amplified with biotinylated primers and pyrosequenced for allele quantification on a Pyromark Q24 system (Qiagen). The same PCR was done on no-reverse transcription control samples to confirm absence of genomic DNA contamination ...
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bioRxiv - Immunology 2020Quote: ... Primers for PCR amplification and sequencing were designed with PyroMark® Assay Design 2.0 software (QIAGEN, Hilden, Germany). PCRs were performed with the IMMOLASE™ DNA polymerase PCR kit (Bioline) ...
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bioRxiv - Cancer Biology 2022Quote: PCR primers for pyrosequencing methylation analysis were designed using the Pyromark Assay Design SW 1.0 software (Qiagen, Germany) (Table 1) ...
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bioRxiv - Microbiology 2022Quote: ... and sgRNA-specific and universal primers (Table 1) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen). sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre) ...
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bioRxiv - Cancer Biology 2023Quote: CXCL5 primer (Qiagen Mm_Cxcl5_2_SG QuantiTect Primer Assay QT01658146 ...
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bioRxiv - Cancer Biology 2023Quote: ... GAPDH primer (Qiagen Mm_Gapdh_3_SG QuantiTect Primer Assay QT01658692)
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bioRxiv - Immunology 2023Quote: ... universal primer (Qiagen) and SYBR Green (Roche ...
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bioRxiv - Developmental Biology 2020Quote: Hsa-miR mimics (339173, miRCURY LNA miRNA Mimic) and inhibitors (339121, miRCURY LNA miRNA Inhibitors) were ordered from Qiagen, Hilden ...
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bioRxiv - Neuroscience 2023Quote: ... and hsa-miR-519a-3p miRCURY LNA miRNA Mimic or its related Negative Control miRCURY LNA miRNA Mimic (Qiagen). Cell lysates were prepared 24 hours later in passive lysis buffer following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... FS RNAs and cellular GAPDH pre-mRNAs were done with the QuantiTect SYBR Green RT-PCR Kit (Qiagen), and performed essentially as described previously (56 ...
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bioRxiv - Microbiology 2021Quote: ... Assays were run in a 384-well format using the QuantiFast Pathogen RT-PCR + IC Kit (QIAGEN; 211454). USA/WA-1/2020 SARS-CoV-2 RNA (20,000 genome copies per reaction ...
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bioRxiv - Bioengineering 2020Quote: ... RT-PCR amplifications were carried out using a Power SYBR Green RNA-to-CT 1 step kit (Qiagen) on a ViiATM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of gene expression level was conducted with Rotor-Gene SYBR Green RT-PCR kit (Qiagen, cat# 204174) on the Rotor-Gene-Q PCR cycler (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: The quantitative real-time TaqMan based assay was carried out using a One-step RT-PCR kit (Qiagen) in the Light Cycler 2.0 system (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR amplifications were carried out using Power SYBR Green RNA-to-CT 1 step kit (Qiagen, UK) on a 384 well ViiA Real-Time PCR System (Applied Bio-systems ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed in an Applied Biosystems QuantStudio 3 using the QuantiTect® Multiplex PCR Kit (QIAGEN). The primers and probes used are listed in Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... All primers were purchased from miScript Primer Assays (Qiagen, Valencia, CA) and GAPDH mRNA expression of each sample was used as normalization.
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bioRxiv - Systems Biology 2019Quote: ... set to collection mode range 125-160bp) to extract the library molecules followed by a concentration step (Qiagen MinElute PCR Purification,. 28004) to produce libraries ready for sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... For RT (Qiagen) for miRNA isolation quality control ...
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bioRxiv - Immunology 2023Quote: ... Primers were designed using the NCBI primer-designing tool (see Table 1) or ordered as validated primers from Qiagen. The mRNA expression levels were plotted as relative gene expression to β-actin (2-ΔCt) ...
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bioRxiv - Genetics 2021Quote: ... The 10 μL reaction was carried out with 40 ng genomic DNA and 0.2 μM of each primer and 5 mM Multiplex PCR Kit (Qiagen). Cycling conditions were identical for both directions with 95°C 15:30 min ...
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bioRxiv - Microbiology 2020Quote: ... which is performed using LRT forward and reverse primers and probe using the Rotor Gene Probe PCR kit (Qiagen) as described above ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of primer mix (with a fluorescently labelled forward primer) at 0.2 μM and 1 μL QIAGEN Multiplex PCR Master Mix (QIAGEN). PCR cycling conditions were 95 °C for 10 min followed by 35 cycles of 95 °C for 30 s ...
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bioRxiv - Cancer Biology 2020Quote: ... Pyrosequencing of the PCR products was performed using 0.5 µM of sequencing primer on the PSQ96 HS System (Pyrosequencing, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: qPCR was carried out using miScript Primer Assays and the miScript SYBR Green PCR kit according to the manufacturer’s instructions (Qiagen) and data were acquired on an ABI PRISM® 7900HT thermal cycler using SDS software ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed on cDNA dilutions of 1/20 in the presence of 7.5-pmol primers with QuantiTect SYBR Green PCR Master Mix (Qiagen) on a Lightcycler 480 (Roche) ...
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bioRxiv - Plant Biology 2021Quote: ... ABI1 and ABI2 with primers listed in Supplemental Table 1 using the Rotor Gene SYBR Green PCR Kit (Qiagen) according to manufacturers’ protocol in a Rotor gene Q cycler (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... The effects of mRNA silencing by siRNA was investigated by real-time PCR using specific QuantiTect primer Assay (Qiagen).
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bioRxiv - Genetics 2022Quote: ... 10 ng of each sample (technical triplicates of 3 independent biological replicates) was amplified using various primers (0.5 µM; Table S2) and SYBR Green PCR master mix (QIAGEN). Reactions were run for 44 cycles on a StepOnePlus Real-Time PCR machine (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Bisulfite-treated DNA was PCR amplified using primers (see Supplementary Table 2) designed with PyroMark Assay Design 2.0 software (Qiagen). Finally ...
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bioRxiv - Cancer Biology 2019Quote: ... and single-cell qPCR of GAPDH or ACTB with QuantiNova SYBR Green PCR Kit (Qiagen, primers see Table S9). The concentration was measured with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Qiagen PyroMark Assay Design 2.0 software was used to design pyrosequencing primers and amplicons were generated with PyroMark PCR Kit (978705, Qiagen) and sequenced using a PyroMark Q96 MD workstation ...