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Citations for Qiagen :
451 - 500 of 5763 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Primers (Qiagen): Gapdh QT00199633 ...
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bioRxiv - Molecular Biology 2021Quote: ... The blood samples from the 2020 FMD outbreak were analysed using a one-step PCR assay using the QIAGEN One-step RT-PCR Kit (Qiagen GmbH, Hilden, Germany) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed on cDNA using a Rotor-Q Real-Time PCR Cycler from Qiagen using gene-specific primers and normalized to the ACTIN gene (listed in Supplemental Table 1).
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bioRxiv - Cell Biology 2022Quote: ... Real time RT-PCR was performed using SYBR Green Fluor qPCR Mastermix (Qiagen, Limburg, The Netherlands) on a CFX Connect (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR of cDNA samples was performed using 2× Rotor-Gene SYBR Green PCR Mastermix (Qiagen) and Rotor-Gene Q real-time PCR cycler (Qiagen).
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bioRxiv - Pathology 2019Quote: ... Quantitative RT-PCR gene expression analysis was performed on duplicate samples using fast SYBR green (Qiagen) technology using the ViiA 7 Real-Time PCR System (Applied Biosystem) ...
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bioRxiv - Pathology 2021Quote: ... a RT-qPCR was performed as quality control step using miRCURY LNA miRNA PCR Assays (Qiagen) to test UniSp6 (Cat No ...
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bioRxiv - Microbiology 2020Quote: All qPCR and RT-qPCR-reactions were done using the QuantiTect Multiplex PCR NoROX Kit (Qiagen) or QuantiTect Probe RT-PCR Kit (Qiagen ...
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bioRxiv - Systems Biology 2021Quote: ... the presence of RVFV RNA was assessed using the QuantiNova® RT-PCR probe kit (QIAGEN). Samples were tested in duplicate using 5 μl of RNA template per well.
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed with a 6plex real-time PCR system (Qiagen, Rotor Gen Q, Germany) using the RealQ Plus 2x Master Mix Green kit ...
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bioRxiv - Immunology 2024Quote: ... Cells were sorted directly into 12.5 μl (per well) of OneStep RT-PCR Buffer mix (QIAGEN) in a 96-well PCR plate (one cell/well).
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bioRxiv - Microbiology 2022Quote: ... We performed purification between the two PCRs to reduce contaminants or carry-over of primer dimers using 96-well PCR purification plates (Qiagen Inc., Germantown, MD, USA). The final libraries were quantified with a Qubit Fluorometer (ThermoFischer ...
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bioRxiv - Neuroscience 2023Quote: ... Adequate sensitivity of the assays was assessed as described earlier using (un)methylated DNA standards from the EpiTect PCR Control DNA Set (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... Adequate sensitivity of the assay was initially confirmed using methylated and unmethylated DNA standards from the EpiTect PCR Control DNA Set (Qiagen).
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bioRxiv - Bioengineering 2022Quote: ... for aliquoting into a 384-well RT2 Profiler PCR Array with genes from the Mouse Inflammatory Response and Autoimmunity set (Qiagen). Plates were analyzed with the Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System ...
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix, 5μL of QIAGEN 5x Q-solution and 2 μL of primer mix). A positive control reaction ...
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bioRxiv - Neuroscience 2019Quote: ... The following primers from Qiagen (Qiagen Quantitect Primer Assay) were used:
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bioRxiv - Cancer Biology 2022Quote: ... The other primers were purchased from QIAGEN (Quantitect Primer): Hprt (QT00166768) ...
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bioRxiv - Neuroscience 2021Quote: ... Primers used were for Esr1 (QuantiTect Primer Assays; Qiagen), Chat ...
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bioRxiv - Cell Biology 2019Quote: ... All primers are QuantiTect primer from Qiagen (Hilden, Germany). Primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Primers were purchased from Qiagen (miScript Primer Assay range) and from Eurogentec (primer list Supp ...
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bioRxiv - Developmental Biology 2023Quote: ... All primers were purchased from Quantitect Primer assay ((Qiagen N.V. ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was conducted using specific 10x QuantiTect primers diluted in 1.1 mL TE pH 8.0 (Qiagen). The genes that were analyzed were Adcy1 (QT00386421) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1μl of primer mix (consisting of the forward and reverse primers of comp162710 at a concentration of 0.2μM) and 1μl of QIAGEN Multiplex PCR Mastermix (Qiagen). The length of amplified fragments was determined by gel electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene-specific primers (supplementary table S9) were used for end-point PCR (HotStarTaq Plus DNA Polymerase; Qiagen), to detect different sized amplicons on agarose gels (1.5%) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were purified and annealed with the sequencing primer for pyrosequencing using the PyroMark Q24 (Qiagen).
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bioRxiv - Immunology 2022Quote: ... and loaded to RT2 Profiler PCR Arrays containing primers for antifungal genes (PAMM-147ZD; table S2; Qiagen). The RNA load was normalized with Actb ...
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bioRxiv - Cell Biology 2021Quote: ... the ViiA 7™ Real-Time PCR system was used to run the Human Stem Cell RT² Profiler™ PCR Array (Qiagen, Hilden, Germany). For the analysis of cardiac differentiation-associated genes ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA quantity was measured using nanodrop and qRT-PCR assays were performed using QuantiNova™ SYBR Green RT-PCR Kit (Qiagen, Cat.# ID: 208154) in StepOnePlus™ Real-Time PCR System by Applied biosystems ...
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bioRxiv - Genetics 2021Quote: ... Amplification products from semi-quantitative RT-PCR were gel-purified using the QIAquick gel extraction kit (Qiagen) and Sanger sequenced ...
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bioRxiv - Cancer Biology 2019Quote: Real time RT-PCR was performed using total RNA isolated on RNeasy Quick spin columns (QIAGEN, CA). One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR ...
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bioRxiv - Genomics 2019Quote: ... Gene expression levels were determined by qPCR using miScript II RT and SYBR Green PCR kits (Qiagen) and results were normalized to the housekeeping gene Hprt1 ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative RT-PCR Array: Total RNA from microfluidic channels was purified using an RNeasy Mini Kit (Qiagen). Next ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA was subsequently transcribed into cDNA using the OneStep RT-PCR Kit from QIAGEN (Catalog # 210212) with the following thermocycler program ...
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bioRxiv - Microbiology 2020Quote: ... A 20 µL reaction mix containing 12.5 µL of 2X Quantitect Probe RT-PCR Master Mix (Qiagen), 0.5 µL of RT mix from the kit ...
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bioRxiv - Microbiology 2020Quote: ... Viral genomic RNA (gRNA) was detected with a one-step real-time RT-PCR assay (Quantifast, Qiagen) using primers and probes generated to target either the SARS-CoV-2 E (28 ...
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bioRxiv - Cell Biology 2021Quote: ... Two micrograms of RNA was used for reverse transcription with Rotor-Gene SYBR Green RT-PCR (QIAGEN) or The SensiMix™ SYBR® No-ROX (BioLine ...
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bioRxiv - Cell Biology 2021Quote: ... Two micrograms of RNA was used for reverse transcription with Rotor-Gene SYBR Green RT-PCR (QIAGEN) or The SensiMix™ SYBR® No-ROX (BioLine ...
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bioRxiv - Immunology 2020Quote: ... Real-time quantitative PCR (RT-qPCR) was performed on a Rotor-Gene Q2plex real-time cycler (Qiagen). For relative gene expressions of IL6 and CXCL8 ...
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bioRxiv - Cell Biology 2021Quote: The RT-qPCR for Miro1 and PCR for mitochondrial DNA was performed on Rotor gene Q (Qiagen). Relative gene expression of Miro1 and mitochondrial DNA copy number were evaluated using their specific forward and reverse primers as indicated in Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... RORC1 and RORC2 gene expression was evaluated by One step SYBR green real-rime RT-PCR (Qiagen) using a Light-Cycler 480 II as follows ...
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bioRxiv - Plant Biology 2023Quote: ... or 500 ng of total RNA for reverse transcription using the One Step RT PCR-Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of RNAs were reverse transcribed and amplified using the OneStep RT-PCR kit (Qiagen Inc.). MTA1 and EF1α (FGRRES_08811 ...
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bioRxiv - Biochemistry 2023Quote: ... The RT-quantitative PCRs were carried out in four biological replicates in a 96-well plate (QIAGEN) using a QIAcuity system (QIAGEN) ...
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bioRxiv - Plant Biology 2020Quote: ... An RNase-Free DNase Set (Qiagen) was used to remove genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Individual set of two siRNAs (Qiagen) against HNF1B or ERG were tested in knockdown efficiency and compared with the siRNA negative-control (Qiagen ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and RNase- Free DNase Set (Qiagen). RNA integrity numbers (RIN ...