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Citations for Qiagen :
451 - 500 of 5692 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Sample was cleaned using a magnetic rack and the supernatant containing DNA was purified with a MinElute PCR Purification Kit (Qiagen). Libraries were prepared according to manufacturer’s instructions using the following kits ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... per condition or genotype were homogenized in 2-ml Eppendorf tubes containing lysis buffer with 1% beta-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless steel beads (Qiagen #69989) ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were centrifuged 15,000 x g at 4°C for 30 min and the resulting supernatant containing the target protein was filtered and purified using a pre-equilibrated Ni-NTA beads column (Qiagen). The recombinant protein was eluted using 4 mL of elution buffer (8 mM Na2HPO4 ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extraction from 50 μl of nuclei suspension from FACS (containing 5000 sorted NeuN+ nuclei) was conducted using RNeasy Mini Kit (74104, Qiagen) and RNase-Free DNase Set (79254 ...
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bioRxiv - Microbiology 2021Quote: ... placed in 250 μL PBS containing a 5 mm stainless steel bead and homogenized using a tissue lyser (Tissue-Lyser II, Qiagen) for 2-minutes at 30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... cDNA MERS-D1 (91) containing basepairs 12259–15470 of the MERS-CoV genome was digested with BgII and purified by Qiagen QIAquick PCR Purification Kit to be used as a standard in the RT-PCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... For tissue disruption frozen biopsy samples were transferred to ceramic bead-filled tubes (#KT03961-1-102.BK, Bertin Technologies, Montigny-le-Bretonneux, France) containing 700 µl QIAazol Lysis reagent (#79306, Qiagen) and homogenized for 2x 20 sec at 5000 rpm using a Precellys 24 tissue homogenizer (Bertin Technologies).
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from each fecal containing tube using the Tissue and Blood DNA Isolation kit (Qiagen, Valencia, CA) following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2021Quote: ... Frozen tissue was partially thawed and submerged in lysis buffer containing 1% ß-mercaptoethanol and 0.5% Reagent DX before tissues were homogenized together with TissueRupture (Qiagen). The homogenate was centrifuged to remove any potential tissue residues ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out in 50-μL total volumes containing 1 μL of template using a DNA HotStarTaq Master Mix kit (Qiagen). The cycling protocol was as follows ...
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bioRxiv - Microbiology 2020Quote: ... and a portion of the tissue (0.08-0.3g) was placed into pre-labeled microcentrifuge tubes containing 5 mm stainless steel beads (Qiagen Inc., CA). Lung samples were homogenized with DMEM + 2% FBS in a TissueLyser (Qiagen Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Individual blood-fed female flies were separated into Eppendorf tubes containing 100 uL of DNA lysis buffer (Gentra Puregene Tissue Kit, Qiagen) and stored at -20°C.
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bioRxiv - Genetics 2022Quote: ... The gRNA was amplified with oligos containing a 5’ T7 promoter sequence and purified using the QIAquick PCR purification Kit (Qiagen). RNA was generated using the Hiscribe™ T7 Quick High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Genetics 2020Quote: ... Bacterial cells lysis was then achieved using Pathogen Lysis Tubes (containing beads) and a lysis buffer in the TissueLyser LT instrument (QIAGEN). Lysates were transferred to QIAamp UCP Mini Columns and bound DNA was eluted in 50 μL of elution buffer ...
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bioRxiv - Genomics 2019Quote: ... For the bacteria-specific 341F/805R47 primer pairs a different reaction mixture was used containing 10x Standard Taq Reaction buffer (Qiagen), 2 mM MgCl2 ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatant was applied twice to a column containing a gel bed of 2 ml nickel-nitriloacetic acid agarose (Qiagen, www.quiagen.com) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads containing long DNA fragments were separated on a magnet and the supernatant containing only small DNA fragments below roughly 800 bp were cleaned up using MinElute PCR purification columns (Qiagen). All libraries were amplified for 12 cycles in total ...
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bioRxiv - Biochemistry 2019Quote: ... Cell lysate was separated by centrifugation at 28000 x g for 45 min and 4°C and the supernatant was loaded onto a column containing 2 mL of Ni-NTA agarose (Qiagen) equilibrated with buffer A for 1 h for purification by nickel affinity chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing His-eIF4A1 or His-eIF4A2 was incubated with a 1.5 ml bed volume of Ni-NTA Superflow (Qiagen, 30430) or a 0.75 ml bed volume of Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue microregions were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
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bioRxiv - Immunology 2020Quote: ... Phage particles were purified from the culture media using PEG/NaCl and uridine containing single-stranded DNA (dU-ssDNA) extracted using a QIAprep spin M13 kit (Qiagen). Mutagenesis was carried out by annealing degenerated oligonucleotides to the dU-ssDNA ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Microbiology 2021Quote: ... Positive clones were verified by Sanger sequencing analysis and the plasmid containing the chimeric vector was purified with a QIAprep Spin Miniprep kit (Qiagen). The chimeric vector containing the f88-4 epitope 4 (aa 662–671 ...
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bioRxiv - Microbiology 2020Quote: ... The DNA template for standard curves was prepared by PCR performed in 50 μl reactions containing 25 μl Taq PCR Mastermix (Qiagen), 19 μl PCR-certified water ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: The validated Alx1DN (N-terminal portion of protein product containing homeodomain and nuclear localization domains) clones in pCS2+8 were purified via miniprep (Qiagen) alongside a control (C-terminal portion containing transactivation domain) ...
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bioRxiv - Genomics 2021Quote: ... The supernatant containing tagmented gDNA was transferred into a new Eppendorf tube followed by DNA purification with MinElute PCR purification kit (Qiagen). Meanwhile ...
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bioRxiv - Microbiology 2021Quote: The resulting sequencing data was analyzed by mapping reads to a database containing 60 bases of sequence flanking each secondary gene using CLC Genomics Workbench (Qiagen). Total read counts at each locus were normalized to the secondary gene with the greatest number of reads in each sample ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2022Quote: ... Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The aqueous phase containing the RNA was transferred into a new tube and RNA extraction was performed by using RNeasy 96 kit (Qiagen). DENV titers were determined by quantitative PCR with reverse transcription assay using TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Genetics 2022Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Genomics 2022Quote: ... 32 μl Master Mix containing 30 μl MDA reaction buffer and 2 μl Phi29 polymerase (REPLI-g UltraFast Mini Kit; Qiagen) were added ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
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bioRxiv - Microbiology 2022Quote: ... at 17,000 G and the pellet containing the microorganisms was used to extract DNA with the DNeasy Blood & Tissue kit as recommended (Qiagen). DNA concentration was estimated by Qubit dsDNA HS kit (Thermo Fisher scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...
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bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR product containing gfp-lcn was recovered from the agarose gel and purified using the gel extraction kit (Qiagen). The purified PCR product was digested with HindIII and BamHI and purified by the PCR purification kit (Qiagen) ...
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bioRxiv - Systems Biology 2022Quote: ... The digested DNA fragment containing the tapA promoter was recovered from the agarose gel and purified using the gel extraction kit (Qiagen). The GFP coding region was amplified by PCR with primers omf316 and om528 using pMF719 (thrC: ...
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bioRxiv - Pathology 2023Quote: Total RNA from HDLEC treated or not with conditioned media containing APLN were harvested 24h after treatment and isolated using the RNeasy mini kit (Qiagen). DNA digestion was performed using the RNase-Free DNase set (Qiagen) ...
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bioRxiv - Biophysics 2022Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the AbundancePCA plasmid and BindingPCA plasmids by temperature-cycle ligation ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...