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Citations for Qiagen :
601 - 650 of 5692 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with buffer containing Proteinase K (PK; Qiagen UK Ltd, Manchester, UK) (1 µg/ml PK ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Synthetic Biology 2022Quote: ... A 150 bp galactose operon-specific fragment containing the region where the predicted RNA 3′-end is located was amplified using HotStarTaq™ DNA polymerase (QIAGEN, Germany) with gal-specific primer M3-F and 3RP primer (Table S3) ...
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bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
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bioRxiv - Genetics 2023Quote: ... amplifications were performed in reaction volumes of 25 μL using a reaction mix containing 1x Type-it Multiplex PCR Master Mix (Qiagen, Gmbh, Hilden), 0.2 μM of each forward and reverse primer pair ...
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bioRxiv - Genetics 2023Quote: ... Frozen tissue samples were homogenised by adding 600 uL RLT containing 1% β-Mercaptoethanol and a 5mm stainless steel bead (Qiagen # 69989) and processing for 40 seconds at 15 Hz with the TissueLyser II ...
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bioRxiv - Immunology 2023Quote: ... Spleens were harvested at indicated timepoints and homogenized in a 2ml homogenizer tube (Fisher Brand Cat. 14-666-315) containing 1ml sterile PBS and one 5mm stainless steel bead (QIAGEN Cat. 69989). For oral infection ...
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bioRxiv - Molecular Biology 2024Quote: ... We typically dissected about 20 slices per HVC and transferred the tissue into an Eppendorf tube containing 340 μl of RLT buffer mixture (with DTT, Qiagen, Valencia, California). We used these rather thin sections for dissection of HVC to avoid contamination of the samples with non-HVC tissues ...
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bioRxiv - Biophysics 2019Quote: ... and probed with primary antibodies anti-5His monoclonal (1:1,000; QIAGEN), anti-Myc monoclonal (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... immunoplates were coated with 0.125ug of Tetra-His antibody (34670; QIAGEN) followed by 2 ug/ml and 5 ug/ml of soluble RBD and NP ...
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bioRxiv - Cell Biology 2021Quote: ... and His6- Vps4 was detected using Penta-His Antibody (Qiagen, Germany) per manufacturer instruction ...
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bioRxiv - Biophysics 2020Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used.
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bioRxiv - Microbiology 2022Quote: ... Antibodies against His-tag and S-tag were purchased from Qiagen and GenScript respectively ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used ...
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bioRxiv - Microbiology 2020Quote: ... An anti·His antibody conjugated to horseradish peroxidase (Penta·His HRP Conjugate, Qiagen) was used to detect 6xHis-tagged proteins ...
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bioRxiv - Cell Biology 2021Quote: ... was from List Biological Laboratories and the mouse monoclonal antibody against the His-tag from Qiagen.
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bioRxiv - Biophysics 2022Quote: The following anti-His fluorophore-conjugated antibodies were purchased from Qiagen and used in UV-Bind assays for His-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Cell Biology 2021Quote: Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... confluent 293T-REx cells (25 cm2) were harvested and nuclei were isolated from 5×106 cells with the Qproteome Cell Compartment Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
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bioRxiv - Immunology 2022Quote: ... for cell fractions ≥0.2×106 cells and the RNeasy Micro Kit (Qiagen) for fractions ≤0.2×106 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cell culture samples were collected with RNAprotect Cell Reagent (Qiagen, Germantown, MD) and the mRNA was extracted using a Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were lysed using Red Blood Cell Lysis buffer (Qiagen) for 1 min ...
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bioRxiv - Genetics 2020Quote: Cell pellets were resuspended in 600 ul Cell Lysis Solution (Qiagen, 158906) supplemented with 3 ul Proteinase K (NEB ...
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bioRxiv - Cell Biology 2023Quote: Cell samples were collected in RNAprotect Cell Reagent (Cat. No. 76526, QIAGEN) during the differentiation procedure at day 9 ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP-positive cells were sorted into REPLI-g cell storage buffer (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... RNAprotect Cell Reagent (Qiagen) was added to stop the infection and preserve the RNA ...
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bioRxiv - Cell Biology 2022Quote: ... coli M15 cells (Qiagen) with pRAREII (Novagen).
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bioRxiv - Molecular Biology 2021Quote: ... coli M15 cells (Qiagen) using H2O-based M9 medium supplemented with 15NH4Cl and D-glucose-13C6-d7 ...
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bioRxiv - Microbiology 2020Quote: ... Fresh frozen tissue homogenates were prepared by thawing frozen tissue and placing 200 mg (± 50 mg) of minced tissue in a tube containing 1 ml DMEM culture medium and a steel bead (Qiagen, Germantown, MD, USA). Homogenization was performed with the TissueLyser LT (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... PI-negative fraction was sorted in each well of a 96 well plate containing 3 μL of PBS (Qiagen, REPLI-g SC Kit) with 0.2% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... followed by lysis in a 2 mL sure-lock tube containing 5 mm stainless steel homogenization beads using the TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 seconds at 30 hz followed by 1 min of 30 hz while keeping the sample cold ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Cell Biology 2022Quote: ... Then midguts were transferred on ice into a 2 ml microtube containing 200 µL of PBS1x with anti-proteases and crushed one minute at 30Hz with a Tissue Lyser (Qiagen, Tissue Lyser LT).
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bioRxiv - Biophysics 2022Quote: ... 100 µl of the protein extract were then supplemented with 400 µl of homogenization buffer containing 10 mM imidazole and added to 50 µl Ni2+-NTA agarose beads (Qiagen GmbH, Hilden, Germany) preconditioned with washing buffer (0.1 M sodium phosphate buffer (pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... washed and lysed with RLT buffer containing β-mercaptoethanol for RNA isolation as per manufacturer’s instructions with Qiagen RNAeasy Micro Kit (Qiagen, Germantown, MD, USA).
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Genomics 2023Quote: ... The still frozen leaf powder was transferred into a 2.0 ml Eppendorf tube containing 400 µl API Qiagen extraction buffer (Qiagen DNeasy Plant Mini Kit). The mixture was incubated at 40°C for 1.5 hours with gentle agitation ...
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bioRxiv - Genetics 2023Quote: Whole-blood samples were collected by venipuncture in tubes containing EDTA and genomic DNA was extracted using the QIAmp DNA Blood Maxi kit (Qiagen, Valencia CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the viral transport media (VTM) containing the nasopharyngeal swabs using the QIAamp® DNA Microbiome Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from ∼1 x 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1x TAE gel electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... pooled cells from each cell population using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturers recommended protocol.
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bioRxiv - Molecular Biology 2019Quote: DNA from H1299 cells (using the Qiagen Blood and Cell Culture Midi Kit) was quantified using a NanoDrop DNA Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells isolated by FACS or cultured cells using the RNEasy Mini kit (Qiagen) or the RNEasy Micro kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... APC-negative cells were single-cell sorted by FACS into TCL buffer (QIAGEN) containing 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from cells with RNeasy kit with Qiashredder cell disruption (Qiagen) and RNase-free DNase Set (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested and resuspended immediately in RNAprotect Cell Reagent (Qiagen, Cat# 76526) for RNA stabilization ...