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Citations for Qiagen :
301 - 350 of 5692 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... 2 mL samples were mixed into glass tubes containing 2 volumes of RNAprotect bacteria reagent solution (Qiagen). After mixing and incubating for 5 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was loaded onto a column containing 1 mL of NiNTA agarose resin (Qiagen, Hilden, Germany) pre-equilibrated with lysis buffer containing 10 mM Imidazole ...
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bioRxiv - Genomics 2020Quote: Tumor samples containing >60% tumor cellularity were selected for DNA extraction using PAXgene Tissue DNA kit (Qiagen). Gentra Puregene Blood Kit (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... The phage-containing pellet was then processed using an Endofree Plasmid Giga Kit (Qiagen, cat. code 12391), with the following adjustments ...
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bioRxiv - Immunology 2022Quote: ... and loaded to RT2 Profiler PCR Arrays containing primers for antifungal genes (PAMM-147ZD; table S2; Qiagen). The RNA load was normalized with Actb ...
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bioRxiv - Plant Biology 2023Quote: ... All PCR reactions were performed in 50µl volume containing: 25µl of Taq PCR Master Mix (QIAGEN, Germany), 2.5µl of BSA (0.2 μg/μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... a control plasmid containing the FKBP12F36V insert was purified from a bacterial clone (QIAGEN Plasmid Maxi kit). Six serial dilutions of the plasmid were generated ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... VG and DRG were transferred into an 1.5ml tube containing one Tungsten Carbide bead (3mm, Qiagen, #69997) and 1 mL of Qiazol lysis reagent (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in 2 mL solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... A master mix containing 12.5 μL RT2 SYBR® Green qPCR master mix (Qiagen, Netherlands, Cat #330503), 0.25 μL forward primer (100 μM) ...
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bioRxiv - Immunology 2024Quote: ... in serum-free media containing a transfection reagent (HiPerFect Transfection Reagent, catalog no. 301704; Qiagen, Valencia, CA). M- or G-MDSC cultures were subjected to a transient elastase treatment (0.4 U ...
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bioRxiv - Microbiology 2024Quote: Tissue samples of mice were transferred into 2 ml tubes containing 4 mm stainless steel beads (Qiagen) and 1 ml of ice-cold sterile saline ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA containing microRNA was isolated according to manufacturer’s guidelines using miRNeasy spin columns (QIAGEN, Maryland, USA). The RNA yield and purity were determined using a NanoDrop ND-1000 (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Molecular Biology 2021Quote: qPCR on cDNA and ChIP samples was carried out in reactions containing SYBR Green I HotStarTaq enzyme (Qiagen). Relative quantitation was performed using standard curves made by pooling aliquots of each cDNA sample being examined ...
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bioRxiv - Physiology 2019Quote: DNA was isolated from intestines containing fecal material using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol for purification of total DNA from animal tissues and for pretreatment of Gram-positive bacteria ...
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bioRxiv - Molecular Biology 2019Quote: ... The peak fractions containing Esco2 were pooled and applied onto a 1 ml Ni-NTA Superflow column (Qiagen), equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... RNA containing aqueous phase of 600 µL was collected from each sample and passed through Qiashredder column (Qiagen) at 21,000 x g for 2 min to homogenize any remaining genomic DNA in the aqueous phase ...
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bioRxiv - Neuroscience 2020Quote: ... containing Protease inhibitor cocktail (PIC, Complete, 1:25) and lysed at 4°C using a TissueLyser LT (Qiagen) on 50 Hz for 2 min ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was homogenized in 1 ml phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (Thermo) and 0.1% RNase A (Qiagen) using a BioMasher (Nippi ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0) containing 15 mg/mL of lysozyme + 15 µL of proteinase K solution (20 mg/mL, Qiagen), and then incubated for 8–10 min ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Cell Biology 2022Quote: ... the aqueous phase containing RNA was mixed with 70% ethanol and transferred to RNeasy MinElute spin columns (QIAGEN). The cleanup and further concentration of the RNA was done using the standard procedure of RNeasy MinElute Cleanup Kit.
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... The upper aqueous phase containing RNA was carefully transferred to a 2 ml collection tube (cat. 990381, Qiagen) without touching the interphase and placed in a QIAcube instrument for extraction.RNA extraction was carried out using the recommended protocol (FIW-50-001-J_FW_MB and PLC program version FIW-50-002-G_PLC_MB ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The supernatant containing the DNA solution was added to an equilibrated Qiagen genomic tip 100 (Qiagen, Venlo, Netherlands). Genomic DNA was eluted and precipitated using isopropanol ...
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bioRxiv - Microbiology 2019Quote: ... The final concentrated solution was transferred to a microtube containing Pathogen lysis tube L beads (Qiagen, Hilden, Germany), 50 μl of Solution SL and 200 μl of sterile saline solution to homogenize ...
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bioRxiv - Molecular Biology 2021Quote: A plasmid containing the modified Widom 603–42 sequence31 was purified with a QIAquick PCR Purification Kit (Qiagen) and used as the template for the amplification.
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bioRxiv - Microbiology 2020Quote: ... RNA was purified from 280 μl VLP-containing supernatant using a QIAamp viral RNA (vRNA) Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Frozen samples of liver and gill tissue were placed together in 600μl of lysis buffer containing 0.5% foaming reagent (Reagent DX, Qiagen) and 1% of ß-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... and placed into 2ml eppendorf tubes containing 200μl LB and a stainless steel bead of 5mm diameter (Qiagen). Flies were homogenized using the TissueLyser II (Qiagen ...
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bioRxiv - Immunology 2019Quote: MDM sorted as described above in “Flow cytometry” were collected into tubes containing RLT buffer (Qiagen, Hilden, Germany) and RNA was isolated using RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... PBS/G was replaced with PBS/G containing 0.2 mg/mL proteinase K (Qiagen N. V., Hilden, Germany) or various concentrations of sodium azide ...
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bioRxiv - Microbiology 2021Quote: Frozen samples of liver and gill tissue were placed together in 600μl of lysis buffer containing 0.5% foaming reagent (Reagent DX, Qiagen) and 1% of ß-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Drops were prepared by mixing 0.1 µl of protein solution with 0.1 µl of precipitant solution containing 0.2 M magnesium chloride and 20% PEG3350 (NeXtal PACT Suite, Qiagen). Crystals were harvested after 2 weeks ...
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bioRxiv - Cancer Biology 2022Quote: ... the aqueous phase containing RNA was mixed with 70% ethanol and transferred to RNeasy MinElute spin columns (QIAGEN). Cleanup and further concentration of the RNA was done using the standard procedure of RNeasy MinElute Cleanup Kit.
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from shoot apexes containing young leaves using RNeasy Plant Mini kit (QIAGEN, Hilden, Germany). RNAseq libraries were prepared using the Illumina TruSeq Stranded RNA LT kit (Illumina ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... 440 μL of MDA reaction mixture containing 290 μL REPLI-g sc Reaction Buffer (Qiagen, catalog no. 150345), 20 μL REPLI-g sc DNA Polymerase (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: All dsDNA was derived from purified plasmid DNA from bacterial cultures containing the indicated plasmid (Qiagen Plasmid Plus) or by SPRI purification of amplified linear dsDNA.
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bioRxiv - Microbiology 2022Quote: ... RNA containing aqueous phase was collected from each sample and passed through a Qiashredder column (Qiagen, Valencia, CA) at 21,000 x g for 2 min to homogenize any remaining genomic DNA in the aqueous phase ...
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bioRxiv - Physiology 2022Quote: ... approximately 100 mg of adipose tissue and a 7 mm metal bead were placed in a 2 mL centrifuge tube containing 0.5 mL PBS buffer (pH 7.4) and homogenised in TissueLyser II (Qiagen) for 2 minutes at 25 Hz ...
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bioRxiv - Immunology 2022Quote: ... 4h and 20h post activation 2000 cells were sorted directly into 0.2ml semi-skirted 96 well PCR plate (Peqlab) containing 10μl 1x TCL Buffer (Qiagen) and 1%(v v-1 ...
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bioRxiv - Developmental Biology 2023Quote: Approximately 15 female flies of CantonS were dissected in 1XPBS before being transferred to tubes containing RNAlater (Qiagen) on ice ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen). Primers ABR-RT-OF and ABR-RT-OR2 were used to sequence the 5’ and 3’ ends of reverse transcriptase ...