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Citations for Qiagen :
51 - 100 of 966 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... S2 cells were transfected with indicated constructs using Effectene transfection reagent (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Extractions using this method were found to be similar in terms of DNA yield and recovery to using the Gene QIAamp 96 DNA QIAcube HT Kit (Qiagen).
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bioRxiv - Systems Biology 2023Quote: Total RNA was isolated from parental MDA-MB-453 and long-term BLU9931 MDA-MB-453 cells using a RNeasy mini kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... coli expression vector pQE80 (Qiagen). Expression of TEV protease and I86-HaloTag-TEV-I87 was induced in BLR (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain M15 [pREP4] (Qiagen) containing a pQE-30 derivative encoding His6-PA1048 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BLR(pREP4) cells (Qiagen) cultured in DYT medium (1% tryptone ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain M15 [pREP4] (Qiagen) containing pAJD2948 was grown in 500 mL of LB broth at 37°C to an OD600 of 0.6-1.0 ...
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bioRxiv - Microbiology 2023Quote: ... coli strain M15 [pREP4] (Qiagen) containing plasmid pAJD3179 encoding His6-PA3978 was grown in LB broth to mid-log phase at 37°C with aeration ...
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bioRxiv - Microbiology 2023Quote: ... coli strain M15 [pREP4] (Qiagen) containing a plasmid pQE-30 protein-encoding derivative was grown in LB broth to mid-log phase at 37°C with aeration ...
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bioRxiv - Biochemistry 2023Quote: ... coli from pQE80L-Navi (Qiagen) as N-terminally His-tagged and biotinylated proteins for SPR ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... S2 cells were transfected with pA-EB1-GFP using Effectene Transfection Reagent (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... pMT-pHluorin2-mCherry was transfected in S2 cells using Effectene transfection reagent (Qiagen) with a 1:10 ratio of DNA to Effectene Reagent.
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bioRxiv - Microbiology 2021Quote: ... into S2 cell lines using Attractene Transfection reagent following the manufacturer’s protocol (Qiagen). A “no miRNA” treatment with only psiCheck-2 plasmid and transfection reagent was also conducted ...
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bioRxiv - Cell Biology 2022Quote: Genes were silenced using siRNAs obtained from Qiagen (see Table S2 for sequences). Silencing was performed for 72–96 h using Lipofectamine RNAiMAX (Thermo Fisher Scientific 13778150 ...
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bioRxiv - Developmental Biology 2021Quote: S2 cells were transfected with Btz-WT or Btz-HD using Effectene (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... using the QIAstat-Dx Respiratory SARS-CoV-2 Panel (Qiagen). Viral stocks were produced infecting at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2021Quote: ... or using the QIAseq SARS-CoV-2 Primer Panel (Qiagen) (for organoid supernatants) ...
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bioRxiv - Genomics 2021Quote: ... QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 333895) and QIAseq FX DNA Library Kit were used in order to prepare amplicon libraries for viral genome sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... with the spike-in control cel-miR-39-3p (QIAGEN, Hilden, Germany) and reverse transcribed (qScript microRNA cDNA synthesis kit Quanta Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... S2 cells were pelleted 48 hours after transient transfection using Effectene Transfection Reagent (Qiagen) and washed in fresh medium ...
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bioRxiv - Genomics 2022Quote: Total RNA from S2 cells was isolated using the RNeasy Plus Mini Kit (Qiagen). Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Plasmids of 5 µg were transfected into 6x10[6] S2 cells using Effectene (Qiagen) and incubated for 3 days ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from S2 cells using the RNeasy Plus Mini Kit (Qiagen 74134). RNA was reverse transcribed using the Maxima H minus system (Thermo Fisher K1681).
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bioRxiv - Evolutionary Biology 2020Quote: ... The pAC5.1-miRNA (300 ng) was transfected into Drosophila S2 cells using Effectene (Qiagen) per the manufactures’ instructions ...
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bioRxiv - Genomics 2021Quote: ... Transient Transfections of S2 cells were performed using Qiagen Effectene Transfection reagent (Qiagen 30425) at a 1:25 DNA-Enhancer ratio ...
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bioRxiv - Cancer Biology 2022Quote: S2-VP10 genomic DNA was extracted using a DNeasy Blood & Tissue Kit (Qiagen, 69504). The promoter and enhancer candidate regions of ROR1 were amplified by PCR using KOD -Plus- Neo (Toyobo ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from S2 cells using the RNeasy Plus Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... or (ii) QIAseq SARS-CoV-2 Primer Panel (Qiagen GmbH, Germany) for amplified of viral genome sequencing or (iii ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with the addition of QIAseq miRNA Library QC Spike-ins for normalization (Qiagen). Adenylation of 3’ linker was performed in a 40 μL reaction at 65°C for 1 hr containing 200 pmol 3’ linker ...
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bioRxiv - Microbiology 2020Quote: ... coli with QIAprep Spin Miniprep Kit (Qiagen) and were confirmed by sequencing ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... coli and purified with NiNTA matrix (Qiagen). Immunization of the rabbits was performed by Absea (Beijing ...
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bioRxiv - Microbiology 2019Quote: ... coli using the QiaPrep Spin kit (Qiagen). DNA samples were analysed by agarose gel electrophoresis and visualised by staining with SYBR Safe (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... coli and purified by Ni-NTA (QIAGEN). Then protein samples were desalted through a PD-10 column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... coli using QIAprep Spin Miniprep kits (Qiagen) as outlined by the manufacturer ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH5α cells and isolated by Qiagen miniprep kit (Qiagen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli DH5α using Plasmid Miniprep Kit (Qiagen) for transformation of B ...
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bioRxiv - Genomics 2020Quote: ... S2 cells were transfected with 1 ug plasmid DNA using the Effectene reagent kit (Qiagen). The plasmid DNA which contains cDNA of FLAG-tagged ECDs are under the metallothionein promoter control ...
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bioRxiv - Molecular Biology 2020Quote: Drosophila S2 cells were transfected with 3μg of pAWM-dfr4 using Effectene transfection kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS using Effectene (Qiagen, Valencia, CA). Cells were harvested 48 hours after transfection and proteins were extracted using EB2 buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: S2 cell transfections were carried out in 6-well plates using Effectene transfection reagent (Qiagen). Each well was transfected with 1 μg of the pAc5.1-λN-HA:dFMRP and 1 μg pAFW-AGO1 or pAFW-GW182 plasmids ...
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bioRxiv - Molecular Biology 2023Quote: S2 cell transfections were carried out in 6-well plates using Effectene transfection reagent (Qiagen). Each well was transfected with 1 μg of the pAc5.1-EGFP:dFMRP and 1 μg of pAc5.1-mCherry plasmids containing DCR1 ...
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bioRxiv - Genomics 2024Quote: ... S2 from 4f-SAMMY-seq was additionally purified using PCR DNA Purification Kit (Qiagen, 28106) and then DNA fragments in this fraction were separated using AMPure XP paramagnetic beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: SARS-CoV-2 RNA was extracted with QIAamp Viral Mini Kit (Qiagen) in a QIACube extractor or with Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... adding UniSp6 and cel-miR-39-3p RNA Spike-Ins (Qiagen Cat. No 339390). qPCR was done using Qiagen miRCURY SYBR Green Kit (Qiagen Cat ...
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bioRxiv - Cell Biology 2020Quote: ... dNTPs and synthetic mRNA Spike-Ins contained in 5 μl of Vapor-Lock (Qiagen). Immediately following sorting ...
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bioRxiv - Neuroscience 2021Quote: ... 3.5ul of miRNeasy Serum/Plasma Spike-In Control (miR-39, 1.6 × 108 copies; Qiagen) was added to each CSF sample ...
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bioRxiv - Genomics 2021Quote: ... Resulting libraries of synthetic RNA spikes were cleaned up using RNeasy spin columns (Qiagen). Synthetic RNA integrity was confirmed by RNA Nano 6000 chip on the Agilent Bioanalyzer.
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bioRxiv - Molecular Biology 2020Quote: ... The resulting pMT-ball-FLAG vector was transfected into S2 cells using Effectene transfection reagent (Qiagen) and stable cell line was generated by following the manufacturer’s instructions ...