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Citations for Qiagen :
451 - 500 of 966 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... then spun at 14000K RPM for 10 minutes at 4°C to pellet debris (Qiagen, 37901). Protein concentration in the supernatant was quantified using BCA assay (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) and total RNA was extracted from the supernatant using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 37°C followed by purification with the QIAquick PCR purification kit (Qiagen). To quantify the RNA transcripts ...
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bioRxiv - Biophysics 2020Quote: ... Enzyme was inactivated (65°C, 15min) prior to PCR purification of the linearized product (Qiagen, Germany). 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Genomics 2021Quote: Skeletal muscle previously stored at −80°C was lysed with the RLT buffer Plus buffer (Qiagen) and beta-mercaptoethanol using the TissueLyser II (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... for 20 min at 37°C and purification with the RNeasy MinElute Cleanup kit (Qiagen, #74204). RNA quality was checked with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: ... snap frozen (−80°C) for proteomics analysis or dissolved in 300 ul of RNAlater reagent (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... at 37°C for 30 min and further purified using an RNeasy Mini Kit (Qiagen, 74104) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Hypothalamic blocks were dissected and stored at − 80°C until RNA extraction using RNAeasy kits (Qiagen).
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bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... tissues were incubated overnight at 56 °C in 300 μL of ATL digestion buffer (Qiagen®) including proteinase K (Cf = 1 mg/ mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were stored at -80°C with the addition of RNA protect reagent (Qiagen, MD, USA). DNA was extracted using DNeasy PowerSoil kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN) (1 h at 50°C and then overnight at 37°C) ...
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bioRxiv - Neuroscience 2023Quote: ... Colonies were cultured for 16 hr at 37°C and DNA was prepared by maxiprep (Qiagen). The entire homology arm sequence and the whole assembled sequence was verified (Genewiz).
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bioRxiv - Neuroscience 2023Quote: ... DNA was eluted two times with 50 μl of pre-heated (70°C) EB (Qiagen, 80204) through centrifugation at 8,500×g for 1 min as documented previously10 ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 30 min followed by clean-up using an RNeasy Mini kit (Qiagen) and the modified transcripts of SINEUP-GFP RNA were recovered.
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, Cat. #28004) to produce tagmented DNA samples ...
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bioRxiv - Plant Biology 2024Quote: ... 65°C treatment with EDTA and cleaned with the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). Depletion of ribosomal RNA (rRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were incubated at 37°C for 15 minutes following addition of 2µg RNase A (Qiagen). 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were washed once with PBS (37 °C) and 350 µl RNA lysis buffer (Qiagen, Buffer RLT
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bioRxiv - Cell Biology 2019Quote: ... and stored at −80°C or immediately used for RNA isolation using RNAeasy Universal Mini kit (Qiagen) with on-column DNAse digestion by RNase-free DNase set (Qiagen ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from -80°C stored stool samples using the DNeasy Powersoil HTP 96 kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated for ~2 h rotating at 4°C with Ni-NTA Resin (Qiagen, 1018244) that had been washed with wash buffer (20mM Tris HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated at 37°C for 30 min after the addition of RNase A (QIAGEN) to a final concentration of 100 µg/mL ...
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bioRxiv - Genomics 2019Quote: ... and grown at 30°C for 18 hours before isolating library vectors using QIAprep (Qiagen, Hilden, Germany) spin miniprep kits.
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bioRxiv - Genomics 2019Quote: ... Cell pellets were either processed immediately or kept at −80°C in RNAProtect Cell Reagent (QIAGEN # 76526).
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bioRxiv - Pathology 2021Quote: ... at 4°C and total RNA was extracted with the RNeasy Midi kit (Qiagen, Santa Clarita, CA). Mouse Genome 430A 2.0 arrays (Affymetrix ...
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bioRxiv - Microbiology 2019Quote: ... lysed with RLT-buffer and frozen at −80 °C for subsequent RNA extraction (RNA easy, Qiagen, Germany), reverse transcription and qPCR ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated for 2h at 4°C with 5 ml of Ni-NTA agarose (Qiagen) pre-equilibrated in wash buffer 1 WB1 ...
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bioRxiv - Microbiology 2020Quote: ... samples were defrosted and incubated in 1 ml of RNAprotect cell reagent (Qiagen, 5 min, 25°C). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... incubated at 37°C for 35 min and were purified with a MinElute PCR Purification Kit (QIAGEN). After the optimization of PCR cycle number using SYBER Green I Nucleic Acid gel Stain (Takara Bio) ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were first equilibrated for at least 48 hours at −20°C in RNAlater ICE™ (Qiagen).
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bioRxiv - Genomics 2022Quote: ... and homogenized at 25/s for 1 min at 4°C using a TissueLyser II (Qiagen, 85300). Samples were incubated at room temperature for 5 min followed by addition of 180 μl chloroform ...
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bioRxiv - Genomics 2022Quote: ... Samples were incubated overnight at 55°C before adding 2 µl of RNaseA (100 mg/ml, Qiagen) and incubated for 15 min at 45°C ...
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bioRxiv - Neuroscience 2023Quote: ... and stored at −80°C until RNA was isolated following the manufacturer’s protocol and DNase (79254, Qiagen) treatment.
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was carried out at 4°C with a Ni-NTA sepharose column (Qiagen, Hilden, Germany). The column was pre-equilibrated with 50 mM NaH2PO4-buffer pH 7.6 ...
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bioRxiv - Systems Biology 2023Quote: ... Cryovials were thawed at 36°C and total RNA was extracted (RNeasy Micro Kit, Qiagen, Hilden, Germany) in one batch ...
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bioRxiv - Microbiology 2023Quote: ... and cleared supernatants stored at −80°C until virus titration/ spin through a QIA shredder column (Qiagen). Viral titers were expressed as TCID50 units per gram source tissue.
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bioRxiv - Microbiology 2023Quote: ... incubated at 65°C for 5 min and beaten for 5 min in a bead beater (Qiagen) set at high speed ...
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bioRxiv - Plant Biology 2024Quote: ... the reactions were carried out at 65 °C for 20 minutes using the Rotor-Gene Q (Qiagen). Melt curve analysis was conducted from 99 °C to 80 °C ...
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bioRxiv - Physiology 2024Quote: ... Organoids were pelleted and stored at −80° C until RNA extraction using the RNeasy Micro Kit (Qiagen). Reverse-transcription was performed with 850 ng of RNA using the High Capacity cDNA RT kit (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 sections were stored in -80°C prior to RNA extraction using the Rneasy micro kit (Qiagen). RNA quality was assessed using a 5300 Fragment Analyzer (Agilent ...