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Citations for Qiagen :
1 - 50 of 966 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The amino acid sequences of coronavirus spike orthologues were subjected to multiple alignment using CLC Workbench 7 (CLC Bio, Qiagen, Aarhus, Denmark). Protein sequence NCBI reference sequences for the indicated viruses are as follows ...
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bioRxiv - Biochemistry 2021Quote: ... or pQE30 vectors (expressed in M15 E. coli cells (Qiagen)) ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg C19orf43 (purified from E. coli BL21 through Qiagen Ni-TA agarose using standard procedures ...
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bioRxiv - Biochemistry 2019Quote: Mutant Chinese hamster BiP proteins with an N-terminal His6-tag were purified as described before with modifications (Preissler et al, 2017b). Proteins were expressed in M15 Escherichia coli (E. coli) cells (Qiagen). The bacterial cultures were grown in LB medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin at 37 ° C to an OD600nm of 0.8 and expression was induced with 1 mM IPTG ...
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bioRxiv - Microbiology 2024Quote: ... Verified plasmids were extracted from Escherichia coli (E. coli) using the QIAprep Spin Miniprep Kit or HiSpeed Plasmid Midi Kit (Qiagen) and dialyzed (0.025µM MCE Membrane ...
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bioRxiv - Microbiology 2020Quote: Coronavirus nsp12 sequence alignment was generated using CLC Workbench (Qiagen) from sequences downloaded from the NCBI website (Accession numbers MN985325.1 and MT123290.1 ...
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bioRxiv - Microbiology 2022Quote: ... 1mg of bacterial genomic DNA (isolated from E. coli cells using genomic DNA kit, Qiagen), or tRNA (from E ...
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bioRxiv - Immunology 2022Quote: ... of soluble SARS-CoV-2 WA1 and SARS-CoV-2 Omicron BA.1 spike trimers were isolated from cell supernatants using a Ni-NTA column (Qiagen, Hilden, Germany). Eluents from Ni-NTA purifications were subjected to SEC using a HiLoad Superdex 200 16/600 column followed by a Superose 6 10/300 (Cytiva ...
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bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... synthetic RNA spike-ins (UniSp2, UniSp4, UniSp5 RNA Spike-in mix; Qiagen) were added and used as quality controls for RNA isolation ...
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bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Bacterial clones on selection plates were scraped and pooled to extract recombinant plasmids containing the recipient barcodes and DNA blocks (donor barcodes, oligonucleotides, and E. coli ORFs) using Plasmid Plus Mini Kit (QIAGEN). The pooling capacity is determined by the number of unique positioning barcodes ...
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bioRxiv - Neuroscience 2021Quote: ... A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) that spanned a wide range of concentrations were added to the recovered RNAs according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) spanning a wide range of molar concentrations were added to the recovered RNAs ...
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bioRxiv - Cell Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen) accord to the instructions provided by the manufacturer.
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... transiently transfected S2 cells using Effectene (Qiagen) were cultured in Schneider’s medium at 22 °C for four days ...
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bioRxiv - Systems Biology 2023Quote: ... 1ul ExiSEQ NGS Spike-in (Qiagen) was added to the extracted RNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 115 in the long-term replication experiment were purified with spin columns (RNeasy, QIAGEN). The purified RNAs were reverse-transcribed using PrimeScript reverse transcriptase (Takara ...
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bioRxiv - Biochemistry 2024Quote: ... coli (QIAGEN) or phage T4 SSB (gp32 ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 specific T cell immune responses were evaluated by QuantiFERON SARS-CoV-2 (Qiagen) [36] ...
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bioRxiv - Molecular Biology 2021Quote: Beta-coronavirus RNA was extracted from the viral samples on each petri dish using the QiAmp Viral RNA mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 whole-genome sequencing was performed by using QIAseq DIRECT SARS-CoV-2 Kit (QIAGEN). The quality of paired-end reads obtained from MiSeq sequencing was analyzed by using Qiagen CLC Genomics Workbench 22.0.1 and the Identify ARTIC V3 SARS-CoV-2 Low Frequency and Shared Variants (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... S2 cells were transfected using Effectene Transfection Reagent (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... S2 cells were transfected using Effectene Transfection Reagent (QIAGEN), as previously described99 with plasmids for Actin5C-GAL4 (a gift from Yasushi Hiromi ...
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bioRxiv - Neuroscience 2023Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Molecular Biology 2022Quote: ... Spike-in cel-miR-39-3p (MS00019789, Qiagen) was used as an internal control.
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (Qiagen) to generate the protein expression strains ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Qiagen). The bacterial cultures were grown in LB medium with 100 μg/ml ampicillin and 50 μg/ml kanamycin at 37°C to an OD600 nm of 0.8 and expression was induced with 1 mM isopropylthio β-D-1-galactopyranoside (IPTG) ...
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bioRxiv - Biophysics 2021Quote: ... coli M15 (Qiagen) was transformed with ApsAC (1-577):pQE70-2Z.4 and grown in LB media to OD600 ≈0.7 ...
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bioRxiv - Systems Biology 2021Quote: RNA isolation from SARS-CoV and SARS-CoV-2 infected A549-ACE2 cells was performed as described above (Qiagen). 500 ng total RNA was used for reverse transcription with PrimeScript RT with gDNA eraser (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... Syn-cel-miR-39 spike in synthetic RNA (Qiagen) was added to monitor extraction efficiency ...
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bioRxiv - Physiology 2022Quote: ... and 0.15 fmol of the spike-in UniSp6 (Qiagen) were added to the cDNA to control for the reproducibility of the cDNA synthesis.
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bioRxiv - Microbiology 2021Quote: Total genomic DNA was isolated directly from long-term parasite cultures using the Gentra Puregene Tissue Kit (Qiagen) according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: GO term enrichment analysis was performed using the gProfileR package (159) and Ingenuity Pathway Analysis (IPA) Software (Qiagen), using expressed genes in each of the normalized dataset as background ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated from cultures infected with rVSV/SARS-CoV-2/GFP1D7 and rVSV/SARS-CoV-2/GFP2E1 using the QIAamp Viral RNA mini kit (Qiagen) and cDNA synthesis was performed using SuperScript III using hexamers (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... coli M15 cells (Qiagen) with pRAREII (Novagen).
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bioRxiv - Biochemistry 2020Quote: ... coli M15 (pREP4) (Qiagen), fresh transformants were inoculated into LB liquid culture (1000 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... coli M15 cells (Qiagen) using H2O-based M9 medium supplemented with 15NH4Cl and D-glucose-13C6-d7 ...
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bioRxiv - Microbiology 2022Quote: ... coli by miniprep (Qiagen), and the internal 504 bp of pilT was removed by digestion with AccI (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... coli strain M15 (Qiagen), and recombinant proteins were produced from the E ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were transfected into S2 cells using Effectene Transfection Reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... DNA constructs were transiently transfected into S2 cells using Effectene (Qiagen). EVs for Western blot analysis (Fig ...
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bioRxiv - Genomics 2020Quote: The SARS-Co-V-2 viral target genome amplicon libraries were constructed using QIAseq SARS-CoV-2 Primer Panel V1 (Qiagen, Germany) coupled with QIAseq FX DNA library kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... Synthetic oligonucleotides obtained from the miRCURY Spike-In kit (Qiagen) were added to the Qiazol lysis buffer before homogenization of the cCM/sEV samples ...
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bioRxiv - Genomics 2021Quote: ... the S1 and S2 plasmids were purified using a GigaPrep kit (Qiagen). To isolate the insert ...
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bioRxiv - Neuroscience 2021Quote: ... and transiently transfected in S2 cells using Effectene (QIAGEN, catalog no. 301425). Cells were collected three days post-transfection ...
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bioRxiv - Cell Biology 2020Quote: Drosophila adult flies or Drosophila S2 cells were homogenised using QIAshredder (QIAGEN) and total RNA was extracted with RNeasy Mini Kit (QIAGEN ...