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Citations for Qiagen :
451 - 500 of 1607 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 200nM siRNA/well were used for transfection using 5µl/well of Hi-Perfect (Qiagen) following manufacturer’s recommendation.
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bioRxiv - Biochemistry 2020Quote: ... but with an anti-His antibody (Qiagen, #34660 at a dilution of 1:5000) as primary ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... His-βC1 fusion proteins were purified using Ni-nitrilotriacetate (Ni-NTA) agarose (Qiagen, 30210) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... N-terminal His-tagged proteins were purified using a QIAexpress protein purification system (Qiagen), as previously described47.
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bioRxiv - Evolutionary Biology 2021Quote: ... His-tagged proteins from the supernatant were incubated overnight with Ni-NTA agarose (Qiagen) at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Blocking of the membranes was performed in anti His HRP conjugate blocking buffer (Qiagen). After blocking at room temperature for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged proteins were purified by affinity chromatography on Ni-NTA Agarose resin (Qiagen) and eluted with buffer containing 0.5 M imidazole ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with pMK33-yki-SBP and pMK33-bon-SBP constructs using the Effectene transfection reagent (Qiagen, Cat# 301427). Stable cell lines were selected with medium containing 300 μg/ml of Hygromycin B (MilliporeSigma ...
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bioRxiv - Cell Biology 2021Quote: Plasmids containing a tagged gene of interest were transfected into Drosophila S2 cells using Effectene transfection reagent following the manufacturer supplied protocol (Qiagen 301425). S2 cells were passaged 24h before transfection ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Genomics 2022Quote: ... 25 fmol of the exogenous synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples at the beginning of the extraction procedure to check both the extraction of miRNAs and the efficiency of the cDNA synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210). Proteins were eluted by 200 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210) as previously described (21) ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21 (DE3) (38) and purified using Ni-NTA affinity column (Qiagen), HiTrapTM Heparin HP column and HiTrapTM Q HP column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21(BE3) and purified using Ni-NTA agarose chromatography (Qiagen) as previously described (Xu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells and purified by FPLC using a Ni-NTA column (Qiagen) and Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified by Nickel affinity chromatography (Ni2+-NTA-sepharose resin, Qiagen). His6-FUBI-eS30 WT and AA constructs were expressed in E ...
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bioRxiv - Bioengineering 2022Quote: ... coli and purified using a QiaPrep Spin Miniprep kit (Qiagen, Venlo, NL). All plasmids were subjected to Sanger sequencing to verify the presence of the correct sing guide RNA (sgRNA ...
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bioRxiv - Microbiology 2023Quote: ... coli was extracted with the MagAttract HMW DNA kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol for DNA extraction from Gram-negative bacteria ...
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bioRxiv - Genomics 2023Quote: ... coli RNA was isolated using an RNEasy column (Qiagen, Germantown, MD, USA) followed by polyadenylation with an E ...
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bioRxiv - Biochemistry 2023Quote: ... coli using CLC Genomic Workbench v9.01 (QIAGEN® CLC bio, Aarhus, Denmark). Furthermore ...
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bioRxiv - Cell Biology 2023Quote: ... coli bacteria (XL1Blue strain or DH5α strain) using plasmid extraction kits (QIAGEN; Venlo ...
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bioRxiv - Developmental Biology 2023Quote: ... coli was modified from the protein purification protocol of The QIAexpressionist (QIAGEN) as follows ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were prepared from VectorBuilder Escherichia coli stocks using Plasmid Miniprep (Qiagen) and ampicillin selection ...
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bioRxiv - Evolutionary Biology 2019Quote: ... from herbarium specimens or silica gel samples (Table S2) using the CTAB modified protocol (Doyle and Doyle, 1987) and the QIAquick PCR Purification Kit (Qiagen, Venlo, Netherlands), followed by Qubit® 2.0 Fluorometer quantification (Life Technologies ...
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bioRxiv - Bioengineering 2019Quote: ... The cut membranes were sandwiched between two O-rings and placed into a commercial miniprep spin column (Figure S2) (Qiagen, Hilden, Germany). The spin column was then placed into a clear 2 mL collection tube.
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bioRxiv - Genomics 2022Quote: ... Total genomic DNAs were respectively extracted using modified SDS-EDTA protocol (Tab. S2) followed by a clean-up step performed with DNeasy Plant Mini Kits (Qiagen, Hilden, Germany). DNA quality and concentration were assessed using a Nanodrop photospectrometer ND-1000 (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen) in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... a 1:2000 dilution of a mouse anti-Penta-His Alexa Fluor 647 conjugate (Qiagen) was used.
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse anti-StrepII and anti-His antibodies were obtained from QIAGEN (catalog number 34850) and Genscript (catalog number A00186) ...
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bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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bioRxiv - Immunology 2022Quote: ... after which the cells were stained with anti-Penta-His-AF647 (1 µg/mL; Qiagen) for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged proteins were purified using the QIAexpress Ni-NTA Protein Purification System (Qiagen) with 0.1 M ...
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bioRxiv - Genomics 2019Quote: ... tBLASTp analyses (e-value < 0.001) were performed using CLC Genomics Workbench (CLC bio Qiagen). Protein sequences were defined as orthologs if they were reciprocal-best BLASTp hits having an e-value < 10−10 ...
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bioRxiv - Molecular Biology 2020Quote: ... after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: The plasmids pIB-spike-V5 with or without pIB-GALNTs-FLAG were transfected to S2R+ cells (DGRC) using Effectene transfection reagent (Qiagen) according to the instructions ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
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bioRxiv - Genomics 2020Quote: ... We used 0.5 ng of total RNA per tissue sample supplied with Cel-mir-39 spike-in (Qiagen, cat# 339390) to perform the reactions in a final volume of 20uL.
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
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bioRxiv - Physiology 2022Quote: ... Total RNA was isolated from 200 μL mouse serum following a standardized protocol (PMID: 24357537) using the miRNeasy Serum Plasma kit with ce-miR-39 spike-in (QIAGEN), QIAcube (QIAGEN ...
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bioRxiv - Neuroscience 2021Quote: ... Purification of C-terminal His6-tag-labeled GluN1 and GluN1ΔM4 by Ni2+-NTA agarose (Qiagen) chromatography was performed as in (Madry et al. ...