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Citations for Qiagen :
301 - 350 of 1607 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The resulting pMT-ball-FLAG vector was transfected into S2 cells using Effectene transfection reagent (Qiagen) and stable cell line was generated by following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... coli and purified using a standard protocol (QIAGEN) at a temperature lower or equal to 12 °C to obtain a near 100 % supercoiled DNA ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Genetics 2022Quote: ... coli and was purified using Ni-NTA (Qiagen) before injection ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells using an RNeasy mini kit (Qiagen) after lysis with lysozyme (FujiFilm Wako ...
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bioRxiv - Biochemistry 2021Quote: ... Escherichia coli SSB (EcoSSB) was purchased from Qiagen.
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bioRxiv - Immunology 2022Quote: ... coli lysate by binding to Ni-NTA (QIAGEN) and gravity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using the MiniPrep Kit (Qiagen), and sequences were confirmed by Sanger sequencing (Quintara Biosciences Service) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli and purified using the MiniPrep Kit (Qiagen), and sequences were confirmed by Sanger sequencing (Quintara Biosciences Service) ...
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bioRxiv - Biochemistry 2023Quote: ... coli and plasmids were recovered by miniprep (Qiagen). Plasmids were checked by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2024Quote: ... coli using the QIAprep Spin Miniprep Kit (Qiagen). All plasmids made by PCR cloning were sequenced by Azenta ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Strep-tag II epitope mouse monoclonal antibody # 34850 (QIAGEN), anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: anti-STrEP-Tag (#34850, Qiagen), monoclonal anti-β-actin antibody (A5441 ...
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bioRxiv - Cell Biology 2022Quote: The plasmid #TH8-77 was digested by Not I and DNA fragment containing cdc8 gene fused with mNeongreen and a 40 aa linker were purified by gel extraction (Qiagen). Obtained DNA fragment was used for the transformation of MBY101 strain (h- ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5 µL of a mixture of spike ins UniSp6 and cel-miR-39-3p (Qiagen) was added to the cDNA reaction ...
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bioRxiv - Microbiology 2019Quote: ... follow manufacturer’s protocol using host and viral primer sets (Table S2) or using QuantiTech Primer Assay (Qiagen) for 18s (Hs-RRN18S_1_SG) ...
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bioRxiv - Cell Biology 2021Quote: ... pMT-FMR1-ADARcd-V5 was co-transfected with pHygro in S2 cells using Effectene transfection reagent (Qiagen) with a 1:10 ratio of DNA to Effectene Reagent ...
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bioRxiv - Immunology 2022Quote: ... and loaded to RT2 Profiler PCR Arrays containing primers for antifungal genes (PAMM-147ZD; table S2; Qiagen). The RNA load was normalized with Actb ...
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bioRxiv - Evolutionary Biology 2024Quote: ... rotundus DNA samples (S2 Table) using the HotStarTaq® Master Mix Kit (Qiagen® Valencia, CA, USA) and primers described by Martins et al [51] at 0.2 µM each ...
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bioRxiv - Microbiology 2023Quote: Protein complexes were produced from the relevant pQE70 plasmid (see Table S2) in strain M15 pREP4 (Qiagen) as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen), which recognized the His-tag on PXDN-con4 ...
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bioRxiv - Immunology 2020Quote: ... immunoplates were coated with 0.125ug of Tetra-His antibody (34670; QIAGEN) followed by 2 ug/ml and 5 ug/ml of soluble RBD and NP ...
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bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
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bioRxiv - Neuroscience 2020Quote: ... cultured and then isolated using a Hi-speed Midiprep kit (Qiagen). Expression was first examined by transfection of N2A cells in vitro ...
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bioRxiv - Cell Biology 2021Quote: ... and His6- Vps4 was detected using Penta-His Antibody (Qiagen, Germany) per manufacturer instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tagged protein was purified using Ni-NTA resin (Qiagen) following the manufacturer’s instructions and was used for rabbit immunization ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed using Ni-NTA agarose (Qiagen). For FRET experiments ...
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bioRxiv - Immunology 2020Quote: ... His-tagged Fabs were loaded onto Ni-NTA superflow resin (Qiagen) preequilibrated with Buffer A (10 mM Tris ...
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bioRxiv - Biophysics 2022Quote: The following anti-His fluorophore-conjugated antibodies were purchased from Qiagen and used in UV-Bind assays for His-tagged proteins ...
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bioRxiv - Molecular Biology 2022Quote: The His-tagged protein was purified by Ni-NTA agarose (Qiagen). Eluted proteins from beads were then applied to the NGC chromatography system (Bio–Rad) ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... His-tagged Usp2-cc was bound to Ni-NTA Agarose (QIAGEN) equilibrated in buffer F (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... rKChIP3-WT (rWT) and rKChIP3-AA (rAA) cells were lysed and total RNA extracted with the RNeasy extraction kit (Qiagen, Netherlands). cDNA was synthesized with Superscript III (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... coli using the QIAprep Spin Miniprep kit (Qiagen, #27104). Genomic DNA (gDNA ...
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bioRxiv - Microbiology 2020Quote: ... coli by affinity chromatography using Ni-NTA agarose (Qiagen) according to the manufacturer’s instructions (Ruc-His6 was purified under urea denaturing conditions) ...
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bioRxiv - Microbiology 2020Quote: ... coli using the QIAprep Miniprep kit (QIAGEN, Germantown, MD) and confirmed by PCR to contain the construct again using the CjNC110F2 and CjNC110R2 primers.
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bioRxiv - Microbiology 2022Quote: ... coli via QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) prior to restriction enzyme digestion ...
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bioRxiv - Microbiology 2021Quote: ... coli with the Qiagen mini plasmid preparation kit (Qiagen). Pneumococcal DNA was isolated by phenol/chloroform extraction and ethanol precipitation from an exponentially growing culture in ThyB media (30 mg/ml Todd-Hewitt Broth powder and 0.2 mg/ml yeast) ...
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bioRxiv - Microbiology 2019Quote: ... coli using the QIAprep® Spin Miniprep Kit (Qiagen) and the DNA was eluted with nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... coli cultures with AllPrep PowerFecal DNA/RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified on Ni-NTA agarose beads (Qiagen) using standard protocols ...
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bioRxiv - Immunology 2022Quote: ... Nanobodies with 6xHis tag were captured by Ni-NTA Agaose (QIAGEN) and eluted by 500 nM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... SUMO-tag and protease were removed using Ni-NTA beads (Qiagen) and the protein of interest dialyzed in a sucrose buffer (250 mM sucrose ...
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bioRxiv - Genomics 2020Quote: ... D.melanogaster S2 cells were transfected with pAc-sgSCR-Cas9 or pAc-sgIntS6-Cas9 by Effectene transfection (Qiagen 301425) and positive clones were selected with puromycin.
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bioRxiv - Molecular Biology 2020Quote: ... Transfection of in vitro-synthesized RNAs into S2 cells was performed using TransMessenger reagent (Qiagen, Valencia, CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN): control Myc ...
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bioRxiv - Microbiology 2022Quote: ... melanogaster S2 pseudo-clonal pools were generated by co-transfection with a pCoPURO (26) by Effectene transfection (QIAGEN). For expression ...
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bioRxiv - Neuroscience 2022Quote: ... and 18S rRNA (5’-GAG CGA AAG CAT TTG CCA AG-3’ and 5’-GGC ATC GTT TAT GGT CGG AA-3’) on a Roter-Gene Q (Qiagen, Hilden, Germany) with 2x AmpiGene® qPCR Green Mix Lo-ROX (Enzo Biochem ...