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Citations for Qiagen :
301 - 350 of 5525 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... genomic DNA from the rostral quarter of the frozen brain was isolated using DNeasy Blood and Tissue kit (69504, Qiagen) and following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Samples were collected from the injured cortical region (including affected as well as non-affected brain tissue) and homogenized using the TissueLyser (QIAGEN). Total RNA was extracted with TRI Reagent (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Vector genomes present in each treated animal was quantified after preparation of genomic DNA from one brain hemisphere with the AllPrep96 DNA/RNA Kit (Qiagen). qPCR with primers and a probe directed against the hSyn promoter sequence (forward 5’-ATGAGTGCAAGTGGGTTTTAGGA-3’ ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from 10-20 mg fresh-frozen postmortem brain tissue using the DNEasy Blood and Tissue kit (cat#: 69504, Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Microbiology 2023Quote: A 388 bp region surrounding NS4A position 19 was amplified from total RNA extracted from a subset of brains using OneStep RT-PCR (Qiagen) using the following primers ...
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bioRxiv - Physiology 2023Quote: Genomic DNA (gDNA) was extracted from brain samples (25 mg) with the DNeasy blood and tissue kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol and resuspended in 20 µl of DEPC water ...
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bioRxiv - Immunology 2024Quote: ... and brain) homogenates collected from mice at 5 dpi and 5 dpc were generated using the Tissue Lyzer II (Qiagen). Briefly ...
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bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Microbiology 2022Quote: ... DNA from human samples was extracted with PowerSoil Pro (Qiagen) on the QiaCube HT (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... the human Stress & Toxicity PathwayFinder RT2 Profiler™ technology (Qiagen), assessing expression of 84 stress response-related genes ...
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Cancer Biology 2022Quote: ... individual Human SHANK3 siRNA_2 (Cat. no. S100717710 Hs_SHANK3_2 siRNA, Qiagen) and individual ON-TARGETplus Human SHANK3 siRNA_7 (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... and Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, Catalog Number ...
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bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: A siRNA library targeting all human kinases (Qiagen, Hilden, Germany) was utilized ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from whole brain of 6 dpf larval zebrafish using RNeasy micro plus RNA extraction kit (Qiagen, Germany). Total RNA (2ng/µl ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from fresh-frozen tissue from four brain regions (motor cortex, frontal cortex, hippocampus, and cerebellum) using the AllPrep DNA/RNA Mini kit (Qiagen, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from brain regions of adult mice using an RNeasy Plus Mini Kit (Cat#74134, Qiagen, Germantown, USA), in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: RNA samples from WT and Rlip+/- mouse liver and brain were prepared using the RNeasy mini kit from Qiagen (Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected CD31+ BECs from the mouse brain using an RNA extraction kit (#74104, Qiagen, MD, USA). The extracted RNA was stored in RNAse-free water at -80°C until transfer to the Boston University School of Medicine Microarray Core Facility ...
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bioRxiv - Genomics 2020Quote: Genomic DNA (gDNA) was extracted from 5154 brain tissue and dural fibroblasts using the MagAttract HMW DNA Kit (Qiagen, Germantown, MD). The length of gDNA was determined by either standard gel electrophoresis in 0.4% agarose or pulse field gel electrophoresis in 1% agarose and 0.5 × TBE for 16 hours at 6 V/cm and a 1200 angle with an initial switch time 1 second and a final switch time-6 seconds ...
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bioRxiv - Genomics 2022Quote: ... we extracted RNA from freshly dissected tissue of two male and two female singing mice (liver and brain) using a Qiagen RNeasy Mini kit (Qiagen 74104). We then homogenized the tissue using a rotor-stator homogenizer with disposable tips and did on-column DNAse digestion following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was removed and mice brains were subjected to DNA purification procedures using the DNeasy PowerSoil Pro Kits (Qiagen, Germany, 47014). DNA purification was followed by qPCR as described below ...
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bioRxiv - Neuroscience 2024Quote: The fresh-frozen brain tissue from control and epileptic animals was homogenized with a handheld rotor-stator homogenizer (QIAGEN, TissueRuptor II) in a lysis buffer that contains 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Neuroscience 2024Quote: RNA extraction was performed on N=31 homogenized half-brain tissue aliquots using the RNeasy Plus Micro Kit (QIAGEN, Germantown, MD). Tissue aliquots were homogenized in 350 µl QIAGEN Buffer RLT Plus using a Qiagen TissueLyser LT and centrifuged for 3 min at maximum speed ...
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bioRxiv - Neuroscience 2023Quote: Sample preparation: 20 mg of snap frozen brain tissues were bead homogenized using 5 mm stainless steel bead (QIAGEN Cat#69989) in 0.4 ml of methanol:water (80:20 v/v ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... or isolated primary mouse macrophages using RNeasy Mini Kit (Qiagen, Cat# 74106) and reverse-transcribed using High-Capacity cDNA Reverse Transcription Kits (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... MiRNA primary quantification was performed in the GeneGlobe Data Analysis Center (Qiagen) for trimming ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissues in the primary cohort were fixed using PAXgene fixative (Qiagen) and prepared for microdissection as described previously [46] ...
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bioRxiv - Biochemistry 2021Quote: ... The primary and secondary antibodies used were anti-penta His (Qiagen #34660) and goat anti-mouse IgG (LiCOR #926-68070 ...
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bioRxiv - Cancer Biology 2019Quote: ... for snap frozen primary specimens and QIAamp DNA FFPE Tissue kit (Qiagen) for FFPE primary specimens ...
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bioRxiv - Cancer Biology 2020Quote: ... primary cultures or fixed tissues using DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from primary hepatocytes using the RNeasy kit (Qiagen). cDNA was synthesized from 2 mg total RNA using oligo (dT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: THP-1 and primary macrophage RNA was extracted using RNeasy kit (Qiagen), followed by cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Human EMT qPCR arrays were purchased from Qiagen (Cat. #: PAHS-021Z), performed as described using RNA from PDX mammary tumors grown in SOFT and STIFF Col1/rBM hydrogels ...
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bioRxiv - Molecular Biology 2021Quote: ... Late female and male L3 instar larvae and 24 h female and male adults were collected separately and their brains (> 20 per sample) dissected in PBS 1X and stored in RNALater (Qiagen, Venlo, Netherlands). Total RNA was isolated using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: Total RNA was isolated from third-instar larval brains or seven-day-old adult heads using the QIAGEN RNeasy Mini Kit (#74106, QIAGEN, MD, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and homogenized using a syringe equipped with a 26G needle (10 brains per sample) using the RNA XS Plus kit (Qiagen, Hilden, Germany). cDNAs were synthesized using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: DNA was extracted from 9mL of stationary growth phase cultures grown in Brain-Heart Infusion (BHI, Oxoid) broth using a QIAsymphony (Qiagen, The Netherlands) with the QIAsymphony DSP Virus/Pathogen Midi Kit according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... ileum and hyperpallial area of the brain) were collected as necropsies after euthanasia and immediately placed into RNA later buffer (Cat. No. 76106, Qiagen, Hilden, Germany), after which they were kept overnight at +4°C and thereafter stored at −80°C until RNA extraction ...
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bioRxiv - Genomics 2022Quote: ... we extracted total RNA from eight snap-frozen tissues kept at -80°C (brain, breast muscle, heart, kidney, liver, lung, spleen, and testis) using the RNeasy Mini Kit (Qiagen; Hombrechtikon, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from primary fibroblasts was extracted using the RNeasy Mini Kit (Qiagen), including DNAse I treatment to remove genomic DNA contamination ...
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bioRxiv - Cancer Biology 2020Quote: FFPE RNA was extracted from matched primary and metastatic FFPE samples by QIAGEN AllPrep DNA/RNA FFPE kit ...
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Molecular profiling of driver events and tumor-infiltrating lymphocytes in metastatic uveal melanomabioRxiv - Cancer Biology 2019Quote: ... Primary eye tumors were sectioned and processed using an FFPE DNA kit (Qiagen). Libraries were made using Illumina TruSeq kit and sequenced on HiSeq2500 instruments at SciLifeLabs in Stockholm or on a NovaSeq at GeneCore SU in Gothenburg ...
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bioRxiv - Cancer Biology 2020Quote: ... and RPR2 (n=6) primary tumors was performed using RNeasy Mini Kit (Qiagen) with the standard protocol ...
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bioRxiv - Neuroscience 2020Quote: RNA from DIV9 primary MSNs was extracted with the miRNeasy micro kit (Qiagen) according to the manufacturer’s instructions ...