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Citations for Qiagen :
201 - 250 of 5525 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from sorted cells and from two replicated of in vitro cultured E-A14 cells stabilized in RLT buffer according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Micro Kit (Qiagen). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from the human cell lines and mouse tumor samples was extracted using RNeasy Plus Mini Kits (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs were extracted from human benign prostatic cells using RNeasy Mini Kit as per manufacture’s protocol (Qiagen, Cat # 74104). cDNA was synthesized using 2µg of RNA using a high-capacity RNA to cDNA kit (applied biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies included: anti-Penta-His (QIAGEN, #34650), anti-FLAG M2 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies (α-His HRP conjugated (Qiagen 34460) or α -VSV-G (Millipore sigma V4888-200UG) ...
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bioRxiv - Immunology 2024Quote: Primary astrocytes were lysed in Buffer RLT (Qiagen) and RNA was isolated from cultured astrocytes using the Qiagen RNeasy Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA for ANXA2 (Human, Qiagen, Hs_ANXA2_8, SI02632385) or siRNA for Anxa2 (Mouse ...
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bioRxiv - Molecular Biology 2021Quote: Cytokine concentration in the brain of mice was determined using a Multi-Analyte ELISArray kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from homogenized brain tissue samples using the RNeasy Lipid Tissue Mini Kit (Qiagen). RNA quantity and quality were measured by Nanodrop® and Agilent 2100 Bioanalyzer technologies ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was extracted from different brain regions using the DNeasy Blood and Tissue Kit (Qiagen), according to manufacturer’s instructions and with the following modifications ...
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bioRxiv - Physiology 2022Quote: Approximately one-half of the powdered brain was homogenized in 1ml of QIAzol lysis reagent (Qiagen) and RNA was isolated using the Direct-zol RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: We extracted RNA from brain tissue using the RNeasy® Lipid Tissue Mini Kit (QIAGEN; 1023539) and ran quality control on all samples using an Agilent 2100 Bioanalyzer system ...
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bioRxiv - Immunology 2021Quote: ... Lungs and brain were homogenised in PBS (10% w/v) using a rotor-star homogeniser (Qiagen) and total RNA extracted using TRIzol (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: Brain tissue from the four specified regions was homogenized using the TissueLyser II (QIAGEN, Germantown, MD) in 10 vol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from frozen brain powder using the Qiagen RNeasy plus kit (Qiagen, 74034). Quality and quantity were assessed with a 4200 TapeStation System (Agilent) ...
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bioRxiv - Genomics 2024Quote: ... Bulk gDNA was extracted from frozen brain samples using the EZ1 DNA Tissue Kit (Qiagen 953034). Five hundred nuclei of each cell type from each brain sample were sorted into 96-well plates with four replicates ...
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bioRxiv - Neuroscience 2023Quote: RNA from pooled fetal brains was extracted using the RNeasy Mini Kit (Qiagen, Catalog no. 74004). RNA quality and integrity were then determined by 28S/18S rRNA analysis with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA from cancer cells (cultured 48 hours in RPMI supplemented with 10% FBS) or primary tumors was extracted using an RNAeasy kit (Qiagen, Germantown, MD), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... The expression levels of the mRNAs were quantified using Human Embryonic Stem Cell RT2 Profile™ PCR Array (Cat. PAHS-081, Qiagen) and RT2 SYBR Green qPCR Master Mix (Cat ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted from human cell lines of WI-38 and MED13LS1497F using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... RT PCR was performed on a Roche LightCycler 96 using the The RT² Profiler™ PCR Array Human Cell Death PathwayFinder (PAHS-212Z, Qiagen)w ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from the proteinase K-digested tail tips of mouse or peripheral blood mononuclear cell (PBMC) of human donors using DNeasy blood tissue kit (Qiagen, #69506). 25 ng of genomic DNA was subjected to bisulfite conversion using a commercial kit (Zymo Research ...
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bioRxiv - Neuroscience 2019Quote: ... Brain tissue of extracted regions (amygdala and prefrontal cortex) were homogenised by TissueRuptor drill (Qiagen, Manchester, UK), with ~90 mg used for RNA extraction ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted total RNA from brain tissue using the Qiagen RNeasy Lipid Tissue Mini Kit (Qiagen, Germany) following manufacturer guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the brains using the QIAGEN RNeasy® Mini Kit (Qiagen, Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Luminex profiling was performed on whole brain tissue homogenates where were homogenized with a TissueLyser LT (Qiagen) for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... DNA was isolated from bulk brain tissue using lysis buffer from the QIAamp DNA Mini kit (Qiagen) followed by phenol chloroform extraction and isopropanol cleanup ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from 5154 brain tissue using the MagAttract HMW DNA Kit (Qiagen, Germantown, MD). The length of gDNA was determined by either standard gel electrophoresis in 0.4% agarose or pulse field gel electrophoresis in 1% agarose and 0.5 × TBE for 16 hours at 6 V/cm and a 1200 angle with an initial switch time of one second and a final switch time of six seconds ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA from brain regions was extracted using an automated nucleic acid extraction platform called QIAcube HT (Qiagen) with a column-based extraction kit ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues dissected from SD and control mice were homogenized in QIAzol Lysis Reagent (Qiagen, Hombrechtikon, Switzerland). RNA was extracted and purified using the QIAGEN miRNeasy mini kit 50 (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... Purification of total RNA from fatty tissues as brain was performed using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA from brain regions was extracted using an automated nucleic acid extraction platform called QIAcube HT (Qiagen) with a column-based extraction kit ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 25 mg brain samples using the DNeasy PowerSoil Pro Kits (Qiagen, Germany, 47014) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA of equal pools of (n = 15) larval brains was extracted using the RNeasy kit (Qiagen). RNA was reverse transcribed to cDNA using the Super Script III First-strand synthesis system (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: The human Hh signaling target PCR array (Qiagen) profiled the expression of 84 key genes responsive to Hh signal transduction ...
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bioRxiv - Microbiology 2021Quote: Human colon resections were placed in RNAlater (Qiagen) at the time of resection ...
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bioRxiv - Microbiology 2023Quote: The human Hippo signaling target PCR array (Qiagen) was used to determine expression of 84 key Hippo target genes ...
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bioRxiv - Microbiology 2023Quote: Human Inflammatory Response & Autoimmunity Focus) (Qiagen, YAHS-205Z) were used for each of the different samples ...
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bioRxiv - Physiology 2024Quote: ... and Qiagen human Primer Assays (Qiagen, Hilden, Germany) specific for FoxO1 and FoxO3 (the latter only in NHBEs) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from cultured from human bronchial epithelial cells / mice right lung tissues and purified using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany), supplemented with the Proteinase K (Qiagen ...
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bioRxiv - Genetics 2019Quote: Total RNA from human cell lines (PTC-05, HepG2 and HEK-293T) was extracted using spin columns with the RNeasy Mini Kit (QIAGEN, GmbH, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of the BCL-2 anti-apoptotic genes in the NPC cell lines were accessed using the custom RT2 Human Apoptosis Profiler PCR array (Qiagen, Hilden, Germany). To delineate the contribution of MCL-1 for cell survival ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA from Amborella generative cells and sperm cells was extracted according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS sorted mouse brain cells stabilized in RNAprotect buffer according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng of cDNA was used for qPCR with the Human Cellular Senescence and Human Aging RT2 Profiler™ PCR Arrays (Qiagen). Arrays were run on the 7300 Real Time PCR System (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA from the control human TSCs as well as WWTR1-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, 74104) per the manufacturer’s protocol with on column DNase digestion ...