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Citations for Qiagen :
301 - 350 of 759 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted 800ng of high molecular weight DNA (Qiagen Genomic-tip 20/G; Qiagen, Germany) from one black New Vineyard female (see below) ...
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bioRxiv - Microbiology 2021Quote: ... a total of 20 μl PCR solution was mixed with 10μl HotStartTaq Master Mix (Qiagen, containing 1 unit of HotStartTaq DNA Polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... 300 mM NaCl and 20 mM imidazole for affinity purification using Ni-NTA resin (Qiagen) as we have done previously (Xu et al. ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL reactions consisted of 1×QuantiFAST reaction mix containing ROX reference dye (Qiagen, Germany), 0.66 µM of forward and reverse primers ...
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bioRxiv - Immunology 2020Quote: ... 0.125 μl 20 μM SmarterR reverse primer (30) and 1 μl PCR-grade H2O (Qiagen). The amplification was performed in a thermal cycler (lid temperature 98°C ...
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bioRxiv - Immunology 2020Quote: ... cells were washed twice with FACS buffer and resuspended in 20 μL TCL buffer (Qiagen) with 1% BME (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by enzyme inactivation 20 minutes at 65°C and column-based purification (Qiagen, #74204). First strand cDNA synthesis was performed with the High-Capacity-RNA-to-cDNA Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 20 g moist sediment using PowerSoil DNA Isolation Kit (Qiagen, USA) and then sequenced using Illumina Novaseq 6000 ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions (20 µl) included 0.5 units of Qiagen HotStartTaq (Cat. No. 203207, QIAgen, Hilden, Germany), 0.2 µM primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from co-cultures after 20 hours using the RNeasy kit (Qiagen). One μg of total RNA was reverse transcribed using iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Pellets were resuspended in RLT buffer supplemented with lysozyme (20 mg/mL; QIAGEN, Hildesheim, Germany) and Zirconium Oxide Beads (0.5 mm ...
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bioRxiv - Microbiology 2019Quote: ... and HRP-penta-His antibody (Qiagen) and HRP-anti-mouse IgG (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...
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bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL of 10 μM double-stranded mature miRNA mimic (Dharmacon) or 20 μM LNA (Qiagen) was mixed with 3 μL lipofectamine RNAiMAX in 100 μL Opti-MEM reduced serum media and added to cells following a 15-minute incubation ...
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bioRxiv - Cell Biology 2019Quote: ... The mixture was washed 20x with 20 ml GST washing buffer on a disposable column (QIAGEN) The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Microbiology 2019Quote: ... DNA extractions for all samples were performed using Qiagen Genomic-tip 20/G (Qiagen, Hildern, Germany) following the manufacturers recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (10 to 20 ng) was retrotranscribed by using the QuantiTect Reverse Transcription Kit (Qiagen). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2020Quote: ... NBS1 and NBS8-organoids at day 20 was conducted using the EpiTec Kit (Qiagen, Hilden, Germany) as described [65] ...
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 37°C followed by purification with the QIAquick PCR purification kit (Qiagen). To quantify the RNA transcripts ...
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bioRxiv - Plant Biology 2021Quote: Approximately 20 mg of pollen grains were ground into fine powder using a TissueLyser II (Qiagen), 5-6 Zirconox beads (2.8 to 3.3 mm ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA from each sample was extracted using the Genomic-tip 20/G column (QIAGEN, Hilden, Germany), following the steps of the yeast DNA extraction protocol provided by the manufacturer ...
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bioRxiv - Physiology 2021Quote: Approximately 20 mg of frozen muscle tissue was homogenized using a TissueLyser II (Qiagen, Valencia, CA) in an ice-cold lysis buffer (1:20 w/v ...
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bioRxiv - Plant Biology 2019Quote: ... 20 seedlings from each sample were frozen and ground to fine powder using a TissueLyser (Qiagen). Homogenised tissue was rigorously suspended in 300 µL of extraction buffer (80% acetone and 2.5mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: Approximately 20 mg of frozen muscle tissue was homogenized using a TissueLyser II (Qiagen, Valencia, CA) in a 1:20 dilution of ice-cold RIPA buffer (pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... 20 mM imidazole] and BiP proteins were bound to 80 µl Ni-NTA agarose beads (Qiagen) for 30 min at 25 °C in the presence of 0.01% Triton X-100 ...
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bioRxiv - Physiology 2020Quote: ... and subsequently homogenized in 300 μl PBS + 0.5% Tween-20 (PBST) using a TissueLyser LT (Qiagen). Next ...
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bioRxiv - Plant Biology 2021Quote: ... 20 min) and purified and DNase treated with RNeasy kit according to the manufacturer’s instructions (Qiagen). RNA integrity was confirmed by agarose gel ...
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bioRxiv - Developmental Biology 2020Quote: ... for 20 min at 37°C and purification with the RNeasy MinElute Cleanup kit (Qiagen, #74204). RNA quality was checked with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Neuroscience 2021Quote: ... and later transcribed into cDNA in a volume of 20 μl using Omniscript reverse transcriptase (Qiagen). The mRNAs of interest were measured using the PowerUp SYBR Green Master Mix (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa PAO1 reference genome and analyzed using RNA analysis module of CLC Genomics Workbench 20 (Qiagen) with default parameters ...
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bioRxiv - Synthetic Biology 2022Quote: ... The pellet was resuspended in G2 Buffer with 20 µL RNase A (100 mg/ml) (Qiagen) and 125 µL RNase Cocktail (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Using the pipeline that was run in CLC Genomics Workbench (GWB) version (v.) 20 (Qiagen, USA), barcodes were removed from raw reads that were previously screened based on phred quality scores ...
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bioRxiv - Microbiology 2022Quote: Analysis of the RNA sequencing results was performed with the CLC Genomics Workbench (version 20; Qiagen). The S ...
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from the abdomens using either a Livak (20) or Qiaquick (Qiagen, Gmbh, Hilden) method ...
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bioRxiv - Pathology 2023Quote: ... Real-time PCR was conducted with cDNA 20 using the Quantitect SYBR Green PCR kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: Total RNA was isolated from 20-30 mg heart tissue (QIAshredder and RNeasy Mini Kit, Qiagen) and eluded in 20 µL RNase-free water ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from approximately 10–20 seedlings using the RNeasy Plant Mini Kit (QIAGEN) followed by DNase I treatment ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... A 180 µL volume of the mixture was then incubated with 20 µL of proteinase K (QIAGEN) at 56°C overnight before a second mechanical lysis was performed ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 mg of skin was homogenized in 20% 0.4M DTT in RLT Lysis Buffer (Qiagen, Hilden, Germany). The RNA was isolated using the RNeasy Plus Micro Kit (cat ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was purified from freshly grown yeast cells using Genomic-tip 20/G column (QIAGEN 10223). DNA library was constructed using the TruSeq DNA PCR-Free Library Prep Kit (Illumina 20015962).
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bioRxiv - Biochemistry 2019Quote: ... Reactions were performed in a total volume of 20 μL using Rotor-Gene Q Detection System (Qiagen), setting the excitation wavelength to 470 nm and detecting emission at 557 nm of the SYPRO Orange protein gel stain ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μl diluted cDNA (1:20) were added to SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) and 10 μM of both forward and reverse primer (Primer Sequences Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... A 20 µL reaction mix containing 12.5 µL of 2X Quantitect Probe RT-PCR Master Mix (Qiagen), 0.5 µL of RT mix from the kit ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...