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Citations for Qiagen :
401 - 450 of 759 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were incubated with biotinylated anti-5His antibodies (Qiagen) and stored for up to 6 months with continuous rotation at 4°C in BRB80 (80 mM Pipes ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Genomics 2022Quote: ... The DNA was eluted from the beads with 50 µL Sample Elution Buffer (Qiagen AE Buffer, 0.1% Tween-20). Qubit dsDNA BR kit was used to quantify the DNA.
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from 20 µL of pooled oocyte culture media using the miRNeasy Serum/Plasma kit (Qiagen) in a final elution volume of 10 µL ...
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bioRxiv - Molecular Biology 2021Quote: HIV-1 viral RNA was extracted from 20-50 ul of plasma using QIAamp Viral RNA mini kit (Qiagen). qRT-PCR was performed using a TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Genomics 2020Quote: ... As described previously,20 RNA from biopsies was isolated using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany), and RNA libraries were prepared using the TruSeq Stranded mRNA protocol (Illumina ...
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bioRxiv - Systems Biology 2019Quote: ... ∼20 mg frozen tissue was pulverised in chloroform-methanol (400 µl; 2:1 v/v) using a TissueLyser (Qiagen), then the mixture was sonicated for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed on cDNA dilutions of 1/20 in the presence of 7.5-pmol primers with QuantiTect SYBR Green PCR Master Mix (Qiagen) on a Lightcycler 480 (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... the reaction mixture in a total volume of 20 μL contained 5.2 μL of PCR Master Mix (components from QIAGEN Corporation ...
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bioRxiv - Plant Biology 2021Quote: ... 18-20 frozen roots in liquid nitrogen were finely ground using glass beads in a Tissue Lyzer II (Qiagen). Subsequently ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue samples were homogenized in 3 mL sterile PBS for 20 seconds on ice using a TissueRuptor homogenizer (Qiagen) with sterile tips ...
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bioRxiv - Biophysics 2021Quote: The soluble fraction of the disrupted cell solution was isolated by centrifugation at 14,000xg for 20 minutes and loaded into a Ni-NTA column system (Qiagen). The column was washed with a volume corresponding to 5 column volumes (CV ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Genomics 2022Quote: ... and 15-20 mg were used to extract RNA using a combination of Trizol and RNeasy Micro Kit (Qiagen), followed by a DNase removal step according to the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Multiplex PCR were run on a Biometra thermocycler in 20 μL reaction volume using HotStarTaq DNA Polymerase (Qiagen, Germany) reagents (i.e ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 20 min under constant rotation with 3,5 ml of pre-washed Ni-NTA superflow beads (Qiagen). Beads were collected by centrifugation ...
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bioRxiv - Developmental Biology 2022Quote: ... A total of 218 proteins that were changed between CDH and control lungs with a p-value <0.05 and log fold change > 1.5 were assessed with pathway analysis via NetworkAnalyst 3.0 20 and Ingenuity Pathway Analysis (IPA) tool (Qiagen Inc) 21.
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bioRxiv - Genomics 2022Quote: ... samples were incubated an additional 30 minutes with 20 µL Proteinase K and 4 µL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit ...
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bioRxiv - Microbiology 2019Quote: DNA was extracted from subsamples frozen at -20°C using the DNeasy PowerLyzer PowerSoil kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions with minor modifications to increase yield and final DNA concentration based on the assumption that some sites and depths would have a relatively low microbial biomass ...
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bioRxiv - Physiology 2019Quote: RNA was purified from 20 mg tissue samples of kidney using the RNeasy Mini Prep kit with DNase (Qiagen) according to manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: Genomic Illumina sequencing reads were assembled into contigs using De Novo Assembly module of CLC Genomics Workbench 20 (Qiagen) with default parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen pieces of tissue (~20 mg) were homogenized in 0.5 ml TRIzol in a TissueLyser II (Qiagen, Hilden, Germany) (2 × 2 min at 30 Hz ...
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bioRxiv - Microbiology 2020Quote: Following 72 h post treatment with a sublethal concentration of compound 33 (3.13 µM), adult male worms (n = 20 worms, three biological replicates) were homogenized with a TissueLyser (Qiagen) and total histones extracted using the EpiQuikTM Total Histone Extraction kit (OP-0006 ...
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bioRxiv - Immunology 2020Quote: ... sorted cells was carried out in a total reaction volume of 20 µl using Sensiscript reverse transcriptase (Qiagen, UK) containing a mix of oligonucleotides dT23VN (NEB ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA of R1 and R2 from 20 DAF siliques was bisulfite treated using EpiTect Bisulfite Kit (Qiagen 59104) following the instructions of manufacturer ...
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bioRxiv - Genomics 2021Quote: ... The concatenated alignment data was used to generate the Neighbor-Joining phylogenetic tree using CLC Genomics Workbench 20 (Qiagen).
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bioRxiv - Immunology 2022Quote: ... Enriched cells were surface stained and 5-20 ×103 intratumoral cDC1s were sorted directly in RLT lysis buffer (Qiagen) containing 2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: High molecular weight (>20 kb fragment) genomic DNA was prepared using the Qiagen MagAttract HMW DNA Kit (Qiagen 67563), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Knockdown of ARRB2/Arrestin3 in CMV:DOR-APEX2 expressing cells was performed using “reverse” transfection in which 20 pmol siRNA (Qiagen) and 12 μL RNAimax (ThermoFisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... High molecular weight DNA was extracted from liver tissue using a Genomic-tip 20/G DNA kit (QIAGEN, 10223).
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bioRxiv - Cancer Biology 2022Quote: ... and 1 µg of each sample was used to prepare 20 µL cDNA using RNeasy Plus Mini Kit (Qiagen). Human RT2 Profiler™ PCR Array ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μg of gDNA were digested with KpnI and passed through a QIAGEN Genomic-tip 20/G column (QIAGEN) to enrich for replication intermediates ...
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bioRxiv - Molecular Biology 2022Quote: ... The final PCR product was purified once more with 1,45x AMPure XP beads and eluted in 20 µL of the Buffer EB (QIAGEN). The libraries were quantified using the KAPA Library quantification kit (Kapa Biosciences ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 ng cDNA were subjected to the Rotor-Gene Q 5-Plex HRM real-time PCR system (Qiagen), using the default program ...
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bioRxiv - Genomics 2023Quote: ... samples were incubated an additional 30 minutes with 20 uL Proteinase K and 4 uL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit.
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bioRxiv - Cell Biology 2022Quote: ... Non-targeting siRNA controls contained 20 nM (for ATGL) or 40 nM (for cPLA2α) AllStars Negative Control siRNA (Qiagen). Transfection complexes were generated using 1 μL/well Lipofectamine RNAiMAX in 24-well plates ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from 20 mg of dried leaf material with the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) using modifications described in Nicholls et al ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Total RNA was extracted from 20 mg of frozen cortex and hippocampus using the RNeasy mini kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... the mouse anti-penta-His antibody (Qiagen; Catalog #34660) was used at a 1:2,000 dilution in 3% BSA in PBS with sodium azide ...
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bioRxiv - Biophysics 2019Quote: ... Biotinylated mouse anti-5xHis antibody was purchase from QIAGEN. Mouse anti Tubulin beta 3 antibody from BioRad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...