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Citations for Qiagen :
601 - 650 of 1798 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Plasmids were isolated with a QIAprep Miniprep Kit (Qiagen) and quantified using 260 nm/280 nm absorbance on a Biotek Synergy H1 plate reader ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Plasmids were extracted via QIAprep spin miniprep kit (Qiagen) and quantitated using absorbance at 260 nm using a Nanodrop spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... A maxiprep was performed to purify the plasmid (Qiagen), and the plasmid subsequently used for stable cell line generation via lentiviral transduction.
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bioRxiv - Genetics 2023Quote: ... plasmids were isolated using Qiaprep spin miniprep kit (Qiagen).
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bioRxiv - Genomics 2023Quote: ... The plasmid was amplified and purified via Maxiprep (QIAGEN). Both TRE-RNASEH1ΔMLS-BFP and BFP-only control vectors were verified by Sanger sequencing at GENEWIZ.
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were extracted using Miniprep kit (Qiagen, Düsseldorf, Germany). Plasmids were linearized and probes were prepared as described in Wilkinson & Nieto (1993 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were isolated using a Spin Miniprep kit (Qiagen). Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with Qiagen miniprep kits (Qiagen GmbH) and verified via Sanger sequencing (Source Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA isolations were performed using plasmid Miniprep kit (Qiagen). All isolations were performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CUBES01 using a Plasmid Mini Kit (QIAGEN, Hilden, Germany) for screening on the presence of the respective plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... and 900ng lentiviral plasmid backbone using PolyFect (Qiagen, 301105) into HEK293t cells in 6-well format according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were isolated using QIAprep Spin Miniprep kits (Qiagen) according to the Miraprep protocol (Pronobis et al. ...
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bioRxiv - Molecular Biology 2023Quote: QIAGEN Plasmid Plus Midi Kit (QIAGEN, cat. No. 12945)
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bioRxiv - Microbiology 2024Quote: ... Plasmids were purified by using Spin Miniprep Kit (Qiagen). Sequencing (Eurofins Genomics ...
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bioRxiv - Biochemistry 2023Quote: ... MN (Germany) or Plasmid Midi kits from Qiagen (Germany).
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bioRxiv - Developmental Biology 2024Quote: ... Expression plasmids were transfected using Effectene transfection reagent (QIAGEN). Plasmids were generated via Gateway® technology (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Microbiology 2022Quote: ... DNA from human samples was extracted with PowerSoil Pro (Qiagen) on the QiaCube HT (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... the human Stress & Toxicity PathwayFinder RT2 Profiler™ technology (Qiagen), assessing expression of 84 stress response-related genes ...
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Cancer Biology 2022Quote: ... individual Human SHANK3 siRNA_2 (Cat. no. S100717710 Hs_SHANK3_2 siRNA, Qiagen) and individual ON-TARGETplus Human SHANK3 siRNA_7 (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... and Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, Catalog Number ...
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bioRxiv - Molecular Biology 2023Quote: A siRNA library targeting all human kinases (Qiagen, Hilden, Germany) was utilized ...
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bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... All constructs were sequenced before use and plasmids used for transfection experiments were purified with the EndoFree® plasmid maxi kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... The ligated cDNA was transformed into XL10-gold and the plasmids were purified using a plasmid plus midi kit (Qiagen, Germantown, MD, USA). The complexity of the cDNA library was 2 × 106 ...
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bioRxiv - Microbiology 2023Quote: ... bacterial colonies were pooled into LB-ampicillin culture media and amplified followed by plasmid purification with the Plasmid Maxi Kit (Qiagen, Cat. No. 12165). Barcode diversity in the plasmid stock was confirmed via next-generation sequencing (Amplicon-EZ – Azenta Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified using QIAprep Spin Miniprep kit (Qiagen) for Sanger sequencing (Macrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was isolated (QIAprep Spin Miniprep Kit, Qiagen, 27104) from each transformant and subsequently 5 μL plasmid DNA (80-100 ng/μL ...
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bioRxiv - Biophysics 2022Quote: ... After purification using a Qiagen Maxiprep plasmid purification kit (Qiagen). The 64x repeat was then cleaved out of the plasmid using XbaI ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were extracted and purified using a Miniprep Kit (QIAGEN), and sequenced (GENEWIZ) ...
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bioRxiv - Developmental Biology 2019Quote: ... using 2µg of plasmid DNA and Polyfect transfection reagent (Qiagen). Positive selection was performed by adding 25µg/ml of hygromycin B (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were prepared for injection using standard miniprep extraction (Qiagen) and submitted for injection (Microinjection service ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmid DNA was purified using QuickLyse Miniprep Kit (Qiagen, USA). The construct containing OGR1 was further confirmed by sequencing using the ABI Prism 377 Automated DNA Sequencer (Applied Biosystems ...
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bioRxiv - Physiology 2019Quote: ... plasmids were extracted using the QIAprep Spin Miniprep kit (Qiagen) and sequenced by ABI 3730 automated DNA sequencer at Davis Sequencing (Davis ...
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bioRxiv - Cell Biology 2019Quote: Plasmids were transfected for 22 hours using Effectene (Qiagen, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Genomics 2019Quote: ... We miniprepped plasmids using the Qiaprep Spin Mini kit (Qiagen) and verified plasmid sequences through Sanger sequencing with the RV3 primer.
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bioRxiv - Genetics 2019Quote: ... DNA maxiprep kit was from Qiagen (EndoFree Plasmid Maxi Kit). All plasmids were cloned with Gibson assembly (42 ...
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bioRxiv - Cancer Biology 2020Quote: ... The phagemid DNA was isolated using plasmid miniprep kit (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... dsDNA was extracted using the Plasmid Miniprep Kit (Qiagen, 27104). dsDNA concentration was measured using a Nanodrop (Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids were purified using the QIAprep spin miniprep kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... Plasmid DNAs were initially isolated using a maxiprep kit (Qiagen). To remove nicked plasmid species ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected in U87 cells with Effectene (Qiagen, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2021Quote: ... isolated with the use of a Plasmid Maxi Kit (Qiagen), and then subjected to in vitro transcription in a transcription mixture of 100 μl containing 10 μg of plasmid as template DNA ...
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bioRxiv - Biochemistry 2020Quote: ... The vector was isolated using Qiagen plasmid maxi kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid and DNA fragments were purified using kits from Qiagen according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... or the pEhEx plasmid backbone using Attractene transfection reagent (Qiagen). Stable transfectants were initially selected at 3 µg/ml Geneticin (ThermoFisher Scientific) ...