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Citations for Qiagen :
451 - 500 of 1798 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... were cloned into pX458 plasmid backbone68 by the Genome Engineering Facility (WIMM, University of Oxford) and purified using Plasmid Midi Kit (Qiagen). pX458 (pSpCas9(BB)-2A-GFP ...
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bioRxiv - Cancer Biology 2020Quote: ... coli TOP10 cells were used again for preparation and amplification of the resulting destination plasmid that was subsequently purified by the Qiagen Plasmid Maxi Kit (Qiagen). The resulting destination plasmid was stored at -20 °C.
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bioRxiv - Synthetic Biology 2021Quote: ... from our assembly reaction transformation into LB and immediately purified their plasmid DNA using the Plasmid Plus Midi Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... We incubated these cultures on a shaker at 225 rpm and 32°C for 12-14 hours then pelleted the cultures by centrifugation and isolated plasmid using the EndoFree plasmid maxiprep kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: 293FT cells were transfected with pMSCV-dnCRTC or pMSCV-GFP constructs together with packaging plasmid pMD.MLV and pseudotyped envelope plasmid pMD2-VSV-G using Effectene transfection reagent (Qiagen #301425) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen) the next day ...
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bioRxiv - Immunology 2022Quote: ... The next day cells were washed off the plates and plasmids were purified using the HiSpeed Plasmid Maxi kit (Qiagen). The resulting replicate libraries were packaged into a lentiviral library using the packaging vectors psPAX2 and pMD2G and the LV-MAX lentiviral production system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 500 ng of pGL3- DARPP-32 plasmids and 50 ng of pRL-SV40 plasmids (renilla; internal reference control) using Polyfect transfection reagent (Qiagen). Lastly ...
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bioRxiv - Genetics 2020Quote: ... the bacterial colonies were scraped into a collection bottle and plasmid pools were extracted with a HiSpeed Plasmid Maxiprep kit (QIAgen). 2 - 3 small plates were prepared in parallel with diluted transformed cells in order to count colonies and confirm the transformation efficiency was sufficient to maintain at least 30x library coverage ...
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bioRxiv - Immunology 2021Quote: ... These plasmids were purified and DNA extracted using QIAGEN Plasmid Midi Kit (100) (cat. n° 12145, QIAGEN, Valencia, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... Cell pellets were collected by centrifugation at 6000xg for 15min and plasmid DNA was isolated using a QIAfilter Plasmid Giga Kit (Qiagen).
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bioRxiv - Genetics 2019Quote: ... Bacterial cells were cultured overnight and plasmid DNA encoding an intermediate library was extracted using QIAGEN Plasmid Midi Kit (QIAGEN). A dsDNA gBlock (IDT ...
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bioRxiv - Genetics 2019Quote: ... Bacterial cells were cultured overnight and plasmid DNA encoding the genome-wide minimized library was extracted using QIAGEN Plasmid Midi Kit (QIAGEN).
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bioRxiv - Genomics 2021Quote: ... Transformation efficiency was determined through serial dilution to ensure sgRNA representation and plasmids were subsequently extracted using Plasmid Mega kits (Qiagen). Library representation was confirmed through sequencing on a NextSeq500 (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... All plasmids were verified by sequencing of the full receptor cDNA and purified with an EndoFree Plasmid Maxi Kit (Qiagen). cDNA sequences of all GPCR variants cloned in pcDNA3.1 are summarized in Data S2.
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bioRxiv - Microbiology 2022Quote: ... coli cells were then grown to isolate the pooled plasmid library by using a Plasmid Midi kit (Qiagen, Germantown, MD) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng pHsp70-MLEfl-eGFP wild type or mutant plasmid was co-transfected with 25 ng plasmid encoding a blasticidin resistance gene using the Effectene transfection reagent (Qiagen). Stable MLEfl-eGFP expressing clones were selected in complete medium containing 25 ng/ml blasticidin for a duration of three weeks ...
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bioRxiv - Systems Biology 2022Quote: ... the bacterial colonies were scraped into a collection bottle and plasmid pools were extracted with a Hi-Speed Plasmid Maxiprep kit (QIAgen). 2-3 small plates were prepared in parallel with diluted transformed cells in order to count colonies and confirm the transformation efficiency was sufficient to maintain at least 20x library coverage ...
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bioRxiv - Molecular Biology 2023Quote: ... the large stocks of recombinant T/F LTR-pGL3 reporter plasmids were generated using Plasmid Maxi kit (Qiagen, Valencia, CA) for immediate use or longer storage at -80 °C.
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bioRxiv - Microbiology 2023Quote: Isolation of EV-D68 Fermon and pGEM plasmids from transformed bacteria were performed using the EndoFree QIAGEN Plasmid Maxi Kit (12362; Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 30°C overnight in presence of a selective antibiotic Ampicillin (100 µg/ml) and isolated the final plasmid via a Midiprep using a Qiafilater plasmid midi kit (Qiagen) the following day.
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bioRxiv - Cancer Biology 2023Quote: ... the lawn of bacteria was scraped off the plates and plasmid DNA was prepared using the Plasmid Plus Maxi kit (Qiagen).
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bioRxiv - Systems Biology 2023Quote: ... the bacterial colonies were scraped into a collection bottle and plasmid pools were extracted with a HiSpeed Plasmid Maxiprep kit (QIAgen). 2-3 small plates were prepared in parallel with diluted transformed cells in order to count colonies and confirm the transformation efficiency was sufficient to maintain at least 30x library coverage.
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bioRxiv - Genetics 2023Quote: ... Bacterial cells were cultured overnight in liquid and plasmid DNA encoding an intermediate library was extracted using QIAGEN Plasmid Maxi Kit (QIAGEN). The vectors were digested with BbsI (NEB).
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bioRxiv - Synthetic Biology 2023Quote: ... cells were scraped from each 96-position array selection plate and a pooled plasmid extraction was performed using Plasmid Plus Mini Kit (QIAGEN). The plasmid DNA was quantified and diluted to ∼ 1ng/μL ...
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bioRxiv - Microbiology 2024Quote: ... Verified plasmids were extracted from Escherichia coli (E. coli) using the QIAprep Spin Miniprep Kit or HiSpeed Plasmid Midi Kit (Qiagen) and dialyzed (0.025µM MCE Membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequences of all constructs were verified by Sanger sequencing (Macrogen) and plasmids were purified prior to use (Midi plasmid kit, QIAGEN).
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Cell Biology 2024Quote: ... falciparum 3D7 ring stage parasites were transfected with 100 μg of purified plasmid DNA (Plasmid Maxi Kit, Qiagen, Valencia, CA) by electroporation (310 V ...
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bioRxiv - Bioengineering 2024Quote: ... Plasmid DNA for plastid transformation was prepared using the QIAGEN Plasmid Maxi Kit (Cat. No 12162; Qiagen inc., Valencia, CA). Leaves were placed abaxial side up on the RMOP medium ...
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bioRxiv - Plant Biology 2024Quote: ... and positive clones (as identified by Sanger sequencing of positive colony PCR products) were used to generate purified plasmid DNA with a Plasmid Midi Kit (Qiagen).
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Cancer Biology 2021Quote: ... the plasmid was extracted using Maxiprep kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... Plasmid preparation kits were obtained from Qiagen (UK). Restriction enzymes were obtained either from New England Biolabs (UK ...
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bioRxiv - Genetics 2021Quote: ... Plasmid was purified using EndoFree Maxiprep kit (Qiagen). Immortalized human aortic endothelial cells (teloHAECs ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were purified using a Miniprep Kit (QIAGEN) and individually sequenced (GENEWIZ) ...
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bioRxiv - Neuroscience 2019Quote: ... and purified with Endofree plasmid maxi kit (Qiagen).
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bioRxiv - Cancer Biology 2019Quote: ... and the plasmid was isolated via Miniprep (Qiagen) prior to being sequence verified using forward and reverse primers.
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bioRxiv - Genomics 2020Quote: Using the QIAfilter Plasmid Midi Kit (Qiagen™), Plasmid DNA for each of the vectors was isolated from cultures of E ...
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bioRxiv - Biochemistry 2020Quote: Cells were transfected with plasmids using Effectene (Qiagen). For the transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with plasmids using Effectene (Qiagen) and with siRNA using HiPerFect (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Plasmid DNA was isolated using miniprep kits (QIAGEN), with modifications for GBS as described elsewhere [49] ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Genomics 2021Quote: ... The plasmid was then purified via maxiprep (Qiagen).
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bioRxiv - Microbiology 2021Quote: Plasmid DNA was isolated using miniprep kits (QIAGEN), with modifications for S ...
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bioRxiv - Microbiology 2020Quote: ... QIAprep Mini Plasmid Prep Kit (QIAGEN, Venlo, Netherlands) was used for isolation of plasmid DNA from bacteria after the principle of alkaline lysis ...
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bioRxiv - Cell Biology 2020Quote: ... using Qiagen Plasmid Midi Kit (Qiagen, MD, USA). This plasmid DNA was then transfected in HEK293T cells with or without miRNA mimics ...
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bioRxiv - Biophysics 2019Quote: ... and the plasmid was isolated via Miniprep (Qiagen) prior to being sequence verified using forward and reverse primers ...
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bioRxiv - Molecular Biology 2020Quote: ... using EndoFree Plasmid Maxi kits (Qiagen; Valencia, CA). Both parasitized erythrocytes and DNAs were suspended in cytomix (120 mM KCl ...