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Citations for Qiagen :
551 - 600 of 2234 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... which was done by hot acid phenol-chloroform treatment and further purified using RNeasy Mini Kit (Qiagen, Hilden, Germany, 74104). RNA stability was determined by agarose gel electrophoresis in 0.8% agarose Tris-Borato-EDTA (TBE ...
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). A polyclonal rabbit antiserum was raised by Labcorp Early Development Laboratories Inc ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany), which purifies RNA and DNA ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding the recombinant RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). On Day 0 ...
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bioRxiv - Genomics 2023Quote: ... Samples were clarified by centrifugation at 2,000 rpm for 10 minutes and subjected to nucleic acid extraction using the cador Pathogen 96 QIAcube HT Kit (Qiagen) in a QIAcube HT automated extractor (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... primary hippocampal neurons were transfected in duplicates or triplicates using 150ng of a plasmid carrying enhanced GFP-gene in combination with 5nmol Power Lock-Nucleic Acid inhibitors (Qiagen) against miR-218-5p ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient cell-free DNA was extracted from 1ml-1.2ml of patient plasma using the QIAamp Circulating Nucleic Acid Kit (QIAGEN # 55114) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Soluble protein was purified using immobilized-metal affinity chromatography (IMAC) by application of the cleared supernatant to nickel-nitrilotriacetic acid (NTA) beads (Qiagen) in a gravity flow column (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant protein was partially purified by affinity chromatography on nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com). Purification steps were carried out as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Frozen pancreas tissue was homogenized using a motorized pestle prior to conducting nucleic acid extraction using the Qiagen AllPrep Universal kit (Qiagen). The processing of all samples was randomized to minimize batch effects attributed to age or sex ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysate (20 mL) was incubated with 10 mL of Ni-NTA agarose (QIAGEN, 30230) for 30 min at room temperature ...
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Pleiotropy in FOXC1-attributable phenotypes involves altered ciliation and cilia-dependent signalingbioRxiv - Genetics 2020Quote: ... 10 μg/ml aprotinin and 10 μg/ml leupeptin) and passed through QIAshredder columns (Qiagen). Obtained protein samples were normalised ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of sample culture was immediately transferred into 2 mL RNA protect bacteria (QIAGEN) and vortexed ...
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit ...
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bioRxiv - Molecular Biology 2019Quote: ... using 100 ng total RNA isolated with the miRNEasy system (QIAGEN) as input ...
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bioRxiv - Evolutionary Biology 2020Quote: ... from 100 mg of infected leaf tissues including a DNaseI (Qiagen) treatment according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and the precipitate was dissolved in 100 μL buffer PB (QIAGEN) for 10 min at 55 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were isolated by injecting 100 μL buffer RLT (Qiagen, 79216) and the lysate was collected at the outflow port into an additional 900 μL of buffer RLT then frozen at −80°C until total RNA extraction using a Qiagen RNeasy Micro Kit ...
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bioRxiv - Neuroscience 2022Quote: ... using Qiagen Genomic-tip 100/G HMW Kit (Qiagen, Hilden, Germany), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 100 µL of viral supernatant was collected in S-Block (Qiagen) previously loaded with VXL lysis buffer containing proteinase K and RNA carrier ...
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bioRxiv - Cell Biology 2019Quote: ... 100 ul Optimem (Thermo-Fisher) and 20 ul Polyfectamine reagent (Qiagen). For co-transfection of two sgRNAs ...
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bioRxiv - Genomics 2022Quote: ... Non-methylated (0%) and fully methylated (100%) standards (Qiagen, Hilden, Germany) were used as controls to examine the consistency of methylation levels across all batches ...
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bioRxiv - Developmental Biology 2023Quote: ... 50,000 sorted cells were resuspended in 100 μL Buffer RLT (Qiagen) containing 1% β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was eluted in 100 µL of elution buffer AE (Qiagen). DNA quality was assessed with NanoDrop™ One (Thermo Scientific™ ...
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bioRxiv - Molecular Biology 2024Quote: ... The following siRNA were transfected at 100 μM using HiPerFect (Qiagen): ATAD5 (HSS129125) ...
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bioRxiv - Genomics 2024Quote: ... gel for 45 minutes at 100 V and gel extracted (Qiagen).
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bioRxiv - Genomics 2022Quote: ... Nucleic acids (DNA/RNA) were isolated from flash-frozen tissues using the All Prep DNA/RNA Mini Kit (Qiagen, Germantown, MD) as previously described [127–129] ...
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bioRxiv - Neuroscience 2019Quote: RNA was isolated from vehicle- or bile acid-treated microglia and astrocyte cultures using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). First-strand cDNA was synthesized by reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Microbiology 2021Quote: Samples were homogenized and nucleic acids were extracted using the MoBio RNA PowerSoil® Total RNA Isolation Kit (Qiagen, CA, USA) and the MoBio RNA PowerSoil® DNA Elution Accessory Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Frozen tissues were weighed and homogenized in RLT and nucleic acids were extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN, #80204) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was divided into two parts: one part was directly combined with nickel-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen, China); the other part was heated in a 65 °C water bath for 60 minutes and then centrifuged at 14000 rpm for 60 minutes ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Microbiology 2022Quote: ... nucleic acid extractions were performed by combining equal amounts of cell culture supernatants with RLT Lysis Buffer (Qiagen, Germantown, MA, USA), with 200 µL of the lysate used for magnetic bead-based extraction according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was adjusted to 500 mM NaCl and 20 mM imidazole before affinity purification by incubation with nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, 30210) for 90 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Lysates of His-tagged α-catenin WT and Δmod were bound to Ni-NTA (Ni2+-nitrilotriacetic acid)-sepharose affinity chromatography (Qiagen), washed with 50 mM Tris pH 7.8 ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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First complete genome characterization of an Indian pigeon pox virus directly from a clinical samplebioRxiv - Evolutionary Biology 2023Quote: ... The extraction of nucleic acid from infected scab samples was performed by using DNeasy blood and tissue purification Kits (QIAGEN, USA) with some modification established by Sarker et al. ...
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bioRxiv - Biochemistry 2023Quote: ... the EcNhaA triple mutant was extracted from membranes with n-Dodecyl β-D-maltoside (DDM; Glycon) and purified by Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) affinity chromatography ...
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bioRxiv - Molecular Biology 2020Quote: 15 ml cell culture was rapidly mixed with 30 ml pre-cooled RNAprotect Bacteria Reagent (Qiagen) placed in an ice bath ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and proteinase K solution (20 mg/mL, Qiagen), followed by an incubation with vortex and lysis steps described in the kit ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Biophysics 2022Quote: ... were filled with ( 1 mL | 0.5 mL ) of bed Ni-NTA resin (Qiagen or Thermo Fisher), and equilibrated with three rinses of Wash buffer (at least 30 resin bed volumes ...
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bioRxiv - Genetics 2022Quote: ... Midi, 3-4 ml of blood) and Puregene (0.3-1 ml of blood, manual extraction) (Qiagen, Cat# 1057048 ...
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bioRxiv - Systems Biology 2022Quote: ... 2 mL cultures were immediately added to centrifuge tubes containing 4 mL RNAprotect Bacteria Reagent (Qiagen), vortexed for 5 seconds and incubated at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: Cells from 1.0 ml of culture were mixed with 2 ml of RNA protect reagent (Qiagen). The mixture was pelleted at 4000 rpm for 10 minutes and then discard the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of each culture was mixed with 1 mL of RNA Bacteria Protect Reagent (Qiagen) immediately after 2 mM ZnCl2 induction (t0 ...