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Citations for Qiagen :
401 - 450 of 551 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The CYP168A1 optimized cDNA insert was removed from the plasmid using NdeI and HindIII restriction enzymes and isolated using agarose gel electrophoresis and the Qiagen Gel Extraction Kit (Qiagen) for ligation into a similarly digested and isolated pCWOri+ CYP expression vector (113) ...
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bioRxiv - Microbiology 2021Quote: ... pLEXSY-Sat2.1-DsRed was digested using SwaI restriction enzyme and the 2.8 kb fragment was gel-purified using the QIAquick Gel Extraction Kit (Qiagen, Germany) followed by ethanol precipitation and resuspension in cytomix transfection buffer (120 mM KCl ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant plasmid DNA was linearized with PstI restriction enzyme and purified with a QIAquick PCR Purification Kit (QIAGEN, Germany). Purified template was in vitro transcribed by RiboMAX™ Large Scale RNA Production System with T7 RNA polymerase (Promega ...
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bioRxiv - Genetics 2020Quote: ... was linearized by restriction enzyme digestion with AgeI and then purified using a MinElute PCR Purification Kit (Qiagen, Duesseldorf, Germany). Cas9 mRNA was produced by in vitro transcription using a mMESSAGE mMACHINE T7 Ultra Kit (Ambion ...
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bioRxiv - Biophysics 2022Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the AbundancePCA plasmid and BindingPCA plasmids by temperature-cycle ligation ...
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bioRxiv - Biophysics 2022Quote: ... The AbundancePCA plasmid and BindingPCA plasmids were all digested by HindIII and NheI enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN). The assembly of AbundancePCA libraries and BindingPCA libraries were done overnight by temperature-cycle ligation using T4 ligase (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... was digested using the same restriction enzyme pair and all digests were gel extracted using QIAquick Gel Extraction Kit (Qiagen). Gel extracts of vector and trib digests were ligated into pCS2+ via overnight incubation at room temperature with T4 DNA ligase according to manufacturer’s instructions (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... We treated the inserts and the pET28PP backbone with the restriction enzymes XbaI and XhoI and then purified the products by gel extraction using the QIAquick Gel Extraction kit (Qiagen). The coding regions were ligated into pET28PP using T4 DNA ligase in T4 DNA ligase buffer (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... A linearized PCR template was generated by digesting a wild-type Hrd1 plasmid with restriction enzyme and purified using a QIAquick PCR purification kit (Qiagen). For each region two separate PCR reactions were set up ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was obtained and amplified in a first-round PCR (RdRpS1 5’-GGKTGGGAYTAYCCKAARTG-3’, RdRpR1 5’-TGYTGTSWRCARAAYTCRTG-3’) using One-Step RT-PCR Enzyme MixKit (Qiagen) with the total expected size of 602 base pairs (bp) ...
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bioRxiv - Biochemistry 2024Quote: ... Nanobody library DNA fragments were digested with the restriction enzymes FseI and Nsil at 37°C for 1 hour and purified using gel extraction (Qiagen). Similarly ...
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bioRxiv - Genomics 2024Quote: ... RNA was first amplified using a first-round PCR (RdRp S1 5ߣ-GGKTGGGAYTAYCCKAARTG -3’, RdRp R1 5’-TGYTGTSWRCARAAYTCRTG-3’) with the One-Step RT-PCR Enzyme MixKit (Qiagen), targeting a total expected size of 620 base pairs (bp) ...
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bioRxiv - Zoology 2023Quote: ... Then 25-50 mg of rehydrated tissue was cut into small pieces with a sterile disposable scalpel blade and transferred into individual 2 ml Eppendorf tubes containing 180 µL of ATL and 20 µL of proteinase K provided by the IndiSpin® QIAcube® HT Pathogen Kit (Qiagen, Courtaboeuf, France). Tubes were incubated between 6 and 12 h at 56°C until full proteolysis was achieved ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were digested for 60 minutes with proteinase K (10 µl) followed by bead-beating for 40 minutes (Vortex-Genie 2 with vortex adapter, Qiagen, Cat: #13000-V1-24) at maximal speed ...
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bioRxiv - Genomics 2021Quote: ... The resultant nuclei were then incubated for 30 min at 37°C with an in-house made Tn5 enzyme and purified with the Qiagen MinElute kit (Qiagen, 28004). PCR reactions for each replicate were performed with 15 cycles using Ad1F and Ad2.2R primers and NEBNext High-Fidelity 2X PCR Master Mix (New England Labs Cat #M0541) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of plasmids pBS-5’UTR-POI-HiBit-3’UTR were linearised by restriction enzyme digest and purified using QIAGEN QIAquick PCR Purification Kit (Qiagen, 28104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid backbones were double-digested with the necessary restriction enzymes and purified using the Qiagen DNA purification kit (#28704X4, QIAGEN GmbH, Germany). DNA inserts were cloned into the digested vectors using the NEBuilder Hifi DNA Assembly Mastermix (#E2621L ...
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bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
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bioRxiv - Microbiology 2021Quote: ... whole ticks were bisected using a sterile 16-gauge needle and incubated in a lysis buffer (i.e., 180 μl Qiagen Buffer ATL and 20 μl proteinase K) at 56°C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.
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bioRxiv - Cell Biology 2024Quote: ... were incubated in a 50 µL reaction with or without triton-x (1% v/v) and with or without proteinase K (Qiagen #19131, final concentration 20 µg/mL) for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and detection was performed using anti-His antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... After incubation with the primary antibody from QIAGEN (Penta-His Antibody, 1:1000 dilution) at RT for 1 h under shaking ...
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bioRxiv - Genetics 2022Quote: ... using biotinylated His5-antibody (Qiagen), as previously described (Asbury et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The PentaHis antibody (Qiagen, Düsseldorf, DE) against His-Tagged proteins was diluted 1:5000 in TBS buffer containing 3% BSA and the membrane was incubated overnight at 4°C followed by 2×10 min washing steps in TBST and 1×10 min in TBS buffers ...
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bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... A mouse anti-GST antibody (Qiagen) diluted (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Biophysics 2021Quote: ... and immunoblotting using anti-Strep antibodies (Qiagen).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... primary mouse anti-His antibody (QIAGEN, 34660), secondary goat anti-rabbit 800CW antibody (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... Tetra·His antibodies (Qiagen, Hilden, Germany; Cat.# 34670), Anti-Mouse IgG (whole molecule) ...
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bioRxiv - Microbiology 2022Quote: ... the mouse monoclonal Penta-His antibody (Qiagen n°34660 stock at 200 µg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... LCAT expression in 293T cells was verified by western blot with an anti-His antibody (Qiagen Penta-His Antibody). LCAT was purified by Ni Sepharose excel (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Microbiology 2020Quote: ... a monoclonal mouse Strep-tag antibodies (#34850, Qiagen), a monoclonal anti-actin antibody (A5441 ...
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bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... analysed by western-blotting using Penta·His Antibody (QIAGEN), of wild-type OxlT measured on the same day of experiment ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies included: anti-Penta-His (QIAGEN, #34650), anti-FLAG M2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated with biotinylated anti-5His antibodies (Qiagen) and stored for up to 6 months with continuous rotation at 4°C in BRB80 (80 mM Pipes ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies (α-His HRP conjugated (Qiagen 34460) or α -VSV-G (Millipore sigma V4888-200UG) ...
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bioRxiv - Biochemistry 2022Quote: ... and HRP–conjugated anti Penta-His antibody (Qiagen). Other chemicals used in this study were of an analytical grade or higher ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...