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Citations for Qiagen :
201 - 250 of 551 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... with 10 μL proteinase K (50 μg/ml, Qiagen, DNeasy Blood & Tissue Kit, #69504) was then added to each tube and incubated with the tail overnight at 60°C ...
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bioRxiv - Genomics 2020Quote: ... Next, >300mAU (500μL of >600 mAU/ml, solution) Proteinase K (Qiagen Cat No. 19131) was added to the sample and incubated at 55° C for 3 hours ...
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bioRxiv - Genomics 2023Quote: Each array was washed as described above and 70 μl of proteinase K (Qiagen) in PKD buffer (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was dissolved in 720μl ATL buffer and 80μl proteinase K (Qiagen, UK) and incubated overnight at 56°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse-crosslinking took place by adding 7.5 μl Proteinase K (0.75 mg/ml; QIAGEN) to the eluate which was then incubated o/n at 65°C ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by proteinase K digestion and DNA isolation with QIAmp DNA Mini Kit (Qiagen), following the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cleavage reactions were stopped by adding 1 μl Proteinase K (20 mg/ml) (Qiagen) and followed by incubation at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... after which the enzymes were removed using a PCR Cleanup Kit (Qiagen). The vector pGEX-4T-LP was digested using the same enzymes ...
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bioRxiv - Microbiology 2023Quote: ... Both enzymes were part of the MagAttract HMW DNA extraction Kit (Qiagen). The tubes were then centrifuged at 15,000 rpm for one minute ...
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bioRxiv - Genetics 2020Quote: ... Beads were then washed with RIPA buffer and treated with RNaseA and proteinase K (Qiagen). DNA was eluted from the beads in Tris-EDTA buffer and cleaned up using the Nucleospin Gel and PCR Purification kit (Macherey-Nagel) ...
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bioRxiv - Genetics 2020Quote: ... ChIPs were incubated with RNAse followed by Proteinase K and DNA purified using QIAquick (Qiagen).
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bioRxiv - Developmental Biology 2020Quote: ... for 2 hours after which samples were treated with 5 μL Proteinase K (Qiagen 19131) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... dsDNA cleavage assay reactions were inactivated with Proteinase K solution (1µl; 20 μg/reaction) (Qiagen) for 20 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 25 uL Proteinase K and 200 uL Buffer AL (DNeasy Blood and Tissue Kit, Qiagen) were added ...
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bioRxiv - Microbiology 2023Quote: ... RNA extractions substituted lysis buffer containing proteinase K solution in PKD buffer (1:16) (Qiagen) for the Mag-Bind beads used in the manufacturer protocol.
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bioRxiv - Cell Biology 2023Quote: ... Eluted DNA was reverse cross-linked and treated with 0.2 mg/ml proteinase K (Qiagen) for 2 h at 60℃ and heat inactive at 95 for 15 min ...
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bioRxiv - Genetics 2023Quote: ... RNA was eluted with a nondenaturing elution and treated with 2ug Proteinase K (Qiagen #19131) for 15 minutes at 55°C followed by the addition of TRIzol Reagent (Invitrogen #15596026 ...
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bioRxiv - Plant Biology 2022Quote: ... The nuclei were embedded in 0.5 % w/v agarose and treated with Proteinase-K (Qiagen) for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... 25 µL proteinase K and 200 µL buffer AL (DNeasy Blood and Tissue kit, Qiagen) were added ...
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bioRxiv - Neuroscience 2022Quote: ... the mouse spinal cord sections were treated with 10 μg/mL proteinase K (QIAGEN, 19131) at 37°C for 5 min and then post-fixed in 4% PFA at RT for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 25 mg tissue were mixed with 20 μl 20 mg/ml Proteinase K (Qiagen, 19131) and 180 μl buffer ATL (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from trypsinized cells using 0.3 μg/μL Proteinase K (QIAGEN #19131) in 10mM Tris pH 8.0 for 1 hour at 55°C ...
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bioRxiv - Microbiology 2022Quote: ... All samples were then treated with RNase A (Omega, #D10SG) and proteinase K (QIAGEN, #160016374), and total DNA was purified by E.Z.N.A ...
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bioRxiv - Microbiology 2023Quote: ... Proteinase K Digestion and Mechanical Disruption of Bacteria (RNAprotect Bacteria Reagent Handbook 01/2020, Qiagen), with 20 μL of proteinase K for each sample and the required volumes for a number of bacteria <7.5 × 108 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 500 µL AP1 lysis buffer and 10 µL proteinase K (10 mg mL-1; Qiagen) were added to each tube ...
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bioRxiv - Immunology 2024Quote: ... samples are then subjected to treatment by Proteinase K and gDNA elimination via columns (QIAgen). Flow-through was mixed with isopropanol and passed over a MinElute (QIAgen ...
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bioRxiv - Systems Biology 2024Quote: ... Tissues were digested from the slide surface by 1h incubation in proteinase K (Qiagen, Germany) at 56°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Individual sections were deparaffinized and tissues were digested with Proteinase K (20 µg/ml; Qiagen) at 37°C for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated for 2h at 56°C in ATL buffer and Proteinase K (Qiagen) to allow complete lyse ...
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bioRxiv - Molecular Biology 2020Quote: ... the RNA was treated on-column by DNase I RNase-free enzyme (Qiagen) according to the kit’s protocol.
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bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
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bioRxiv - Microbiology 2024Quote: ... products were digested with restriction enzymes (Table 2) and purified (Qiagen PCR purification). Following triple ligation into pJB38 ...
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bioRxiv - Systems Biology 2024Quote: ... extending the incubation with the DNase enzyme (RNase-Free DNase Set, QIAGEN S.r.l.) to 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... The DhaA31 enzyme containing 6xHis-tag was purified using Ni-NTA columns (Qiagen) and eluted with 8 mL of elution buffer (50 mM Na2HPO4 ...
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bioRxiv - Genetics 2024Quote: ... The restriction-enzyme-digested gene blocks were also purified (Qiagen PCR Purification Kit). Each digested gene block was ligated into the digested FCRL3 plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA fragments were treated with Proteinase K and recovered with a QIAquick PCR purification kit (Qiagen). Validation of the DRIP was performed by qPCR ...
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bioRxiv - Microbiology 2021Quote: ... K-means clustering was performed in R and functional analysis was performed using IPA [18] (QIAGEN Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μg/ml proteinase K) for DNA extraction using a QIAamp® DNA Mini Kit (Qiagen). DNA samples were used for bacterial profiling with high-throughput 16S rRNA amplicon sequencing and quantitative polymerase chain reactions.
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bioRxiv - Genomics 2020Quote: ... We subjected samples to a two day 0.5 EDTA and proteinase K (10 mg/mL; Qiagen) digestion ...
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bioRxiv - Genomics 2020Quote: ... The nuclei were embedded in 0.5 % w/v agarose followed by treatment with proteinase-K (Qiagen) for 2 h ...
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bioRxiv - Immunology 2021Quote: ... and treated with proteinase K before isolating DNA using the QIAquick PCR Purification kit from Qiagen. DNA content from chromatin IPs was assessed using SYBR Green amplification with the following primers designed from the promoter region of the murine CXCR6 gene ...
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bioRxiv - Microbiology 2021Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and proteinase K solution (20 mg/mL, Qiagen), followed by an incubation with vortex and lysis steps described in the kit ...
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bioRxiv - Microbiology 2020Quote: ... crystals were flash frozen (100 °K) in the above reservoir solution supplemented with 10% glycerol (QIAGEN). The diffraction data set for complexes of Brd4(BD1 ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen) in Bionano Lysis Buffer according to the Bionano Prep Cell Culture DNA Isolation Protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were transferred to fresh sterile micro centrifuge tubes and Proteinase K (Qiagen DNeasy® Kit) was added followed by incubation overnight at 56°C ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% Triton X-100 and 200 µg/ml proteinase K) or DNA Blood Mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN) (1 h at 50°C and then overnight at 37°C) ...
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bioRxiv - Microbiology 2023Quote: ... using Proteinase K-mediated lysis followed by column purification using the DNeasy PowerLyzer Microbial Kit (Qiagen). These seven samples were sent to Microbial Genome Sequencing Center (MiGS ...