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Citations for Qiagen :
251 - 300 of 551 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The next day tissues were removed from the slide using proteinase K (Qiagen, cat. no. 19131) and cDNA was released from the glass slides and collected in low-binding tubes for cDNA purification and second-strand synthesis ...
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bioRxiv - Genomics 2022Quote: ... 300 µL lysis solution (LSDNA, RevoluGen) and 20 µL of 20 mg/mL Proteinase K (Qiagen) was added to the partly-lysed cells and incubated at 56 °C for 20 min ...
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bioRxiv - Biophysics 2020Quote: ... The enzyme was purified through a nickel-nitrilotriacetic acid column (Ni-NTA superflow, Qiagen), which was followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Genomics 2022Quote: ... DNA was further treated with 50 µg/ml of RNase enzyme (Qiagen, Hilden, Germany) for 1 h followed by phenol/chloroform extraction ...
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bioRxiv - Microbiology 2020Quote: ... A 180 µL volume of the mixture was then incubated with 20 µL of proteinase K (QIAGEN) at 56°C overnight before a second mechanical lysis was performed ...
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bioRxiv - Genomics 2021Quote: ... Ligated chromatin was de-crosslinked by incubation with 4μL proteinase K (20mg/mL, Qiagen, Cat. 19131, Germany), overnight for 65°C and 300 RPM) ...
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bioRxiv - Genomics 2021Quote: ... Decrosslinking of chromatin was performed using proteinase K (included in the Qiagen DNeasy blood and tissue kit) at 65 °C for > 4 hr ...
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bioRxiv - Microbiology 2020Quote: ... Proteinase K (20 uL) and buffer AL (200 uL) from the QIAamp DNA Mini Kit by QIAGEN was added prior to the samples undergoing mechanical bead beating (MagNA Lyser Instrument ...
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bioRxiv - Evolutionary Biology 2020Quote: ... proteinase K digestion and RNA removal were performed using a DNeasy Blood & Tissue kit (Qiagen, The Netherlands) and isolation of gDNA was carried out with a Wizard Genomic DNA Purification Kit (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 mg/mL proteinase K) and DNA clean up (MiniElute reaction clean up kit, 28204, Qiagen). qPCR was performed using mPer2 and Wnt3a primers ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM NaCl in UltraPure distilled water) with 0.4 mg/ml Proteinase K (QIAGEN N.V., Venlo, NL). Homogenized samples were incubated at 37 °C for 1.5 h followed by 98 °C for 2 min ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was resuspended in 340μl of lysis buffer containing 5 mM EDTA and proteinase K (Qiagen) and incubated at 55°C overnight ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... whole male mosquitoes were individually incubated with 20µL of proteinase K and 180µL of G2 buffer (Qiagen) at 56°C for one hour then were homogenized using tungsten beads and a TissueLyser (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... RNase-A was degraded by the addition of proteinase-K (3 mAU/mL, 19131, Qiagen, Hilden, Germany) and inverting the tube briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... The immunoprecipitated DNA was treated with RNaseA and proteinase K and recovered using the PCR purification kit (Qiagen). The DNA was then subjected to quality control with Qubit Fluorometer and TapeStation ...
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bioRxiv - Genomics 2022Quote: ... After overnight proteinase K digestion in Lysis Buffer (Bionano Genomics) and one hour treatment with RNAse A (Qiagen), plugs were washed four times in 1x Wash Buffer (Bionano Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was purified by RNase A and proteinase K treatments and recovered using a DNA purification kit (Qiagen). For ChIP-qPCR ...
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bioRxiv - Genomics 2020Quote: ... including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). Samples were labelled with cy3 using a One-Colour Labeling Kit (Roche-Nimblegen) ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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bioRxiv - Microbiology 2020Quote: ... pH 8.0) containing 15 mg/mL of lysozyme + 15 µL of proteinase K solution (20 mg/mL, Qiagen), and then incubated for 8–10 min ...
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bioRxiv - Microbiology 2022Quote: ... The phage protein capsid was digested with Proteinase K and the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) was used to purify the DNA ...
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bioRxiv - Microbiology 2021Quote: ... prior to the addition of proteinase K and 5 μl of 100 mg ml−1 RNase A (Qiagen). The DNA concentration was determined using a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PBS/G was replaced with PBS/G containing 0.2 mg/mL proteinase K (Qiagen N. V., Hilden, Germany) or various concentrations of sodium azide ...
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bioRxiv - Systems Biology 2023Quote: ... followed by mechanical disruption by glass bead beating and digestion in proteinase K (Qiagen Sciences, Germantown, MD, USA). DNA was extracted and purified using the MagNA Pure nucleic acid purification platform (Roche Diagnostics Corp. ...
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bioRxiv - Microbiology 2022Quote: ... 20 μL of EDTA (final concentration 20 mM) and 1.3 μL of Proteinase K (20 mg/mL) (Qiagen) were added and incubated at 56 °C for 1.5 h ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments were released by addition of 15 µL 10% SDS and 30 µL Proteinase K (Qiagen, #19133) to each tube followed by incubation at 50°C for 3h ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then digested using proteinase K in buffer ATL (20 mg/mL, >318 mU/mL, Qiagen) overnight at 56°C ...
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bioRxiv - Microbiology 2024Quote: ... the homogenates were incubated overnight at 56°C with 20 µL of 20 mg/mL protease K (Qiagen). The crude extract was purified using the QIAamp DNA Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Pellets were then incubated for 3 h at 37°C with 2ml of 5 mg/ml SDS in 75 mM Tris pH 7.5 with 0.33 mg/ml proteinase K (Qiagen). At this point ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by enzyme inactivation 20 minutes at 65°C and column-based purification (Qiagen, #74204). First strand cDNA synthesis was performed with the High-Capacity-RNA-to-cDNA Kit (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digests were performed after plasmids were isolated using a midiprep kit (QIAGEN, 27104). All riboprobes were generated from linearized plasmids and synthesized using the DIG RNA Labelling Kit (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products and enzyme-digested vectors were purified using QIAquick PCR Purification Kit (Qiagen), and the resulting vectors were transformed into ECOSTM Competent E ...
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bioRxiv - Microbiology 2022Quote: ... FITC-negative and non-sorted fractions immediately after cell sorting using the QIAmp DNA Microbiome kit (Qiagen) and a FastPrep-24 Classic homogenizer (MP Biomedicals ...
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bioRxiv - Genetics 2021Quote: ... to which were then added 180 µl of the kit’s Buffer ATL and 30 µl of Proteinase K (Qiagen) at 20 mg/ml ...
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bioRxiv - Genomics 2020Quote: ... Contaminants were removed from DNA by RNaseA and Proteinase K treatment followed by purification with PCR purification kit (QIAGEN).
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bioRxiv - Cancer Biology 2020Quote: DNA isolation was performed by adding proteinase K and incubation at 56°C using QIAamp DNA Mini Kit (Qiagen). DNA was quantified using a Qubit Fluorometer (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 μg of mHTTex1 seed or 50 μg of seeded neuronal lysate was incubated with varying doses of proteinase K (0 to 0.5 μg/mL, Qiagen) for 30 min at 25°C and heat inactivated at 75°C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The samples were RNase-A and protease-K treated and finally eluted using the Qiagen PCR purification kit (Qiagen). The following antibodies were used (4 μg/sample) ...
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bioRxiv - Plant Biology 2021Quote: ... the protein was removed by 1.1 μL of proteinase K (20mg/ml) at 45° C for 1h and RNA by 2μL of RNaseA (1mg/ml) (Qiagen) digestion at 37°C for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... 35 nL cell lysis buffer was added to each microwell (P1: 2.89 AU/L Protease K (19155, Qiagen, Germany) and 72.8 mM pH 7.5 Tris•HCl (15567027 ...
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bioRxiv - Genomics 2022Quote: ... samples were incubated an additional 30 minutes with 20 µL Proteinase K and 4 µL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit ...
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bioRxiv - Immunology 2021Quote: ... treated with proteinase K and RNase A and then purified using QIAquick PCR purification kit (QIAGEN, Cat no. 28104). DNA libraries were prepared using two biological replicates of each TF ChIP and sequenced on Miseq Nano (Fasteris Life Sciences ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Microbiology 2022Quote: ... The nucleases were then denatured by simultaneously adding 20 μl of 0.5 M EDTA and 57 μL of 20 mg/μL Proteinase K (Qiagen) and incubating at 56°C for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by incubation with 6 ml of lysis buffer (50 mM Tris pH 8.0, 50 mM EDTA, 1% SDS, 0.1 mg/ml proteinase K (Qiagen, 19131)) at 55°C for 16 hours ...
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bioRxiv - Genomics 2023Quote: ... samples were incubated an additional 30 minutes with 20 uL Proteinase K and 4 uL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit.
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bioRxiv - Synthetic Biology 2022Quote: ... single flies were homogenized in 30μL Tris-EDTA buffer pH 8.0 with 5M NaCl and Proteinase-K (proK) Qiagen solution (from Qiagen #69504). Samples were incubated at 37°C for 30 minutes and then at 95°C for four minutes ...
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bioRxiv - Genomics 2023Quote: ... After overnight proteinase K digestion in Lysis Buffer (BNG) and 1-hour treatment with RNAse A (Qiagen, MD,USA), plugs were washed 4 times in 1×Wash Buffer (BNG ...
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bioRxiv - Biochemistry 2023Quote: ... the bead slurry was spun down and resuspended in 100 µl wash buffer and digested with proteinase K (Qiagen) at 50 °C for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...