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Citations for Qiagen :
401 - 450 of 615 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... coli in LB Broth with 100ug/mL ampicillin using Qiaprep Spin columns (Qiagen).
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bioRxiv - Microbiology 2019Quote: ... coli host JM109 and isolated with a commercial plasmid isolation kit (Qiagen Inc.). For cloning PCR fragments ...
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bioRxiv - Developmental Biology 2020Quote: ... coli cells were isolated according to the QIAprep Spin Miniprep Kit protocol (Qiagen). A restriction reaction was then set up to linearize the plasmid DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... coli cells and positive colonies were collected using a Mini Prep kit (Qiagen) and sequenced using the U6 primer (Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... coli strain TJ249 culture using the QIAGEN DNeasy Blood & Tissue kit (QIAGEN GmbH). The extracted genomic DNA was sequenced on the PacBio RS II platform using P6C4 chemistry at Macrogen (Tokyo ...
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bioRxiv - Biophysics 2023Quote: ... coli and purified using a combination of Ni-NTA affinity (Qiagen, Hilden, Germany) and size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... coli BL21 ClearColi cell expression system and purified by NiNTA-Agarose beads (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from Escherichia coli using a Spin MiniPrep kit (Qiagen) and transferred to X ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL of plasma/EV suspension were mixed with 1000 μL Qiazol and 1 μL of a mix of 3 synthetic spike-in controls (Qiagen, Germany). After a 10-minute incubation at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
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bioRxiv - Genomics 2022Quote: ... The sample was incubated at room temperature for 5 min and 3.75μL (25 fmol final concentration) of the synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples ...
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bioRxiv - Microbiology 2020Quote: ... BipA-His was purified from the soluble fraction by affinity chromatography using Ni-NTA agarose (QIAGEN) previously equilibrated with equilibration buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 6x-His-Smt3 was purified from BL21 E.coli cells using a Ni-NTA affinity column (Qiagen) following manufacturer indications ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Plant Biology 2021Quote: ... HIS-tagged proteins were purified by metal-affinity chromatography using Ni-NTA resins (Qiagen, Hilden, Germany). Protein concentration was estimated by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and His-tagged Fabs were isolated from cell supernatants using Ni-NTA columns (Qiagen, Hilden, Germany). IgGs ...
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bioRxiv - Cancer Biology 2019Quote: ... 50 nM siRNA/well were used for transfection using 0.8 µl/well of Hi-Perfect (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Biochemistry 2024Quote: ... Proteins modified with 6×-His-Ub was purified using Ni-NTA Sepharose (142350243, Qiagen, Alameda, CA) on a rotating platform for 2 hours at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Marinkelle at the Universidad de los Andes (see Table S1 in Supplementary Material) by using a DNeasy Blood & Tissue kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and 26 Lycaeides from the Dubois hybrid zone (Table S1) was isolated with Qiagen’s MagAttract High-Molecular-Weight (HMW) DNA extraction kit (cat. No. 67563; Qiagen Inc., Valencia, CA, USA). The concentration of DNA was assessed using the dsDNA HS assay on a Qubit 4 fluorometer (Thermo Fisher).
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH10B strains using a QIAprep plasmid miniprep kit (Qiagen, Redwood City, CA, USA) and digested with PvuII (FastDigest ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) and purified with dextrin sepharose high performance (Qiagen, Chatsworth, CA, USA) using an affinity column (Qiagen) ...
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bioRxiv - Genetics 2020Quote: ... coli cultures by maxi-prep using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965) and quantified plasmid DNA using Qubit dsDNA HS Assay Kit (Thermo Fisher #Q32854) ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
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bioRxiv - Microbiology 2021Quote: ... coli was performed using the QIAprep Spin Miniprep Kit (QIAGEN Inc, Valencia, CA, USA). Oligonucleotides were purchased from Sigma-Aldrich (St ...
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bioRxiv - Microbiology 2020Quote: ... coli and BCG cultures were treated with RNAprotect Cell Reagent (Qiagen, CAT No.:76526) before lysis ...
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bioRxiv - Plant Biology 2020Quote: ... coli Rosetta cells was carried out using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) as described in Barja and Rodríguez-Concepción (2020) ...
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bioRxiv - Biophysics 2021Quote: ... coli bacteria and isolation from the cells using a QIAprep Spin Miniprep Kit (Qiagen), the plasmid was linearized by restriction enzyme digestion at the last restriction site RS6 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells and plasmids were isolated using standard miniprep kits (Macheray & Nagel, and Qiagen). Sequences of integrative plasmids can be found in Supplementary Table 4.
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bioRxiv - Immunology 2022Quote: ... of soluble SARS-CoV-2 WA1 and SARS-CoV-2 Omicron BA.1 spike trimers were isolated from cell supernatants using a Ni-NTA column (Qiagen, Hilden, Germany). Eluents from Ni-NTA purifications were subjected to SEC using a HiLoad Superdex 200 16/600 column followed by a Superose 6 10/300 (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
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bioRxiv - Biophysics 2022Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... The JAK3 PCR fragment was purified and cloned into the pQE-Tri System His-Strep2 vector (Qiagen). The final pQE-JAK3 construct was used to transfect GC using the Xfect transfection kit (Takara Bio ...
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bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged Sic1PY conjugates (polyubiquitylated Sic1PY, Ubn-Sic1PY) were purified by incubating with Ni-NTA resin (Qiagen) at 4 °C for 1 h ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... 0.2 µg of AFP1-His proteins was incubated with 20 µl Ni-NTA agarose beads (Qiagen, Germany) in buffer (25 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... Purification of His-tagged GA-MatryoshCaMP6s was performed with Ni-NTA agarose beads (Qiagen, cat. No: 30210). Before applying the lysate ...