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Citations for Qiagen :
201 - 250 of 615 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to Ni-NTA beads (Qiagen). The beads were washed with 100 mL lysis buffer and protein was eluted with 40 mL elution buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... coli and purified using a standard protocol (QIAGEN) at a temperature lower or equal to 12 °C to obtain a near 100 % supercoiled DNA ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Genetics 2022Quote: ... coli and was purified using Ni-NTA (Qiagen) before injection ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells using an RNeasy mini kit (Qiagen) after lysis with lysozyme (FujiFilm Wako ...
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bioRxiv - Biochemistry 2021Quote: ... Escherichia coli SSB (EcoSSB) was purchased from Qiagen.
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bioRxiv - Immunology 2022Quote: ... coli lysate by binding to Ni-NTA (QIAGEN) and gravity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using the MiniPrep Kit (Qiagen), and sequences were confirmed by Sanger sequencing (Quintara Biosciences Service) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli and purified using the MiniPrep Kit (Qiagen), and sequences were confirmed by Sanger sequencing (Quintara Biosciences Service) ...
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bioRxiv - Biochemistry 2023Quote: ... coli and plasmids were recovered by miniprep (Qiagen). Plasmids were checked by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2024Quote: ... coli using the QIAprep Spin Miniprep Kit (Qiagen). All plasmids made by PCR cloning were sequenced by Azenta ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Strep-tag II epitope mouse monoclonal antibody # 34850 (QIAGEN), anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: anti-STrEP-Tag (#34850, Qiagen), monoclonal anti-β-actin antibody (A5441 ...
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bioRxiv - Microbiology 2019Quote: Transformation with the plasmids identified in Table S1 was conducted by electroporating isolated plasmid DNA (Qiagen) into 50 μl competent cells at 1.8 kV ...
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bioRxiv - Synthetic Biology 2021Quote: ... with primers pBEST_LinL_F and pBEST_LinL_R (Table S1) and subsequent purification using QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... 0.5 µL of a mixture of spike ins UniSp6 and cel-miR-39-3p (Qiagen) was added to the cDNA reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen), which recognized the His-tag on PXDN-con4 ...
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bioRxiv - Immunology 2020Quote: ... immunoplates were coated with 0.125ug of Tetra-His antibody (34670; QIAGEN) followed by 2 ug/ml and 5 ug/ml of soluble RBD and NP ...
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bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
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bioRxiv - Neuroscience 2020Quote: ... cultured and then isolated using a Hi-speed Midiprep kit (Qiagen). Expression was first examined by transfection of N2A cells in vitro ...
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bioRxiv - Cell Biology 2021Quote: ... and His6- Vps4 was detected using Penta-His Antibody (Qiagen, Germany) per manufacturer instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tagged protein was purified using Ni-NTA resin (Qiagen) following the manufacturer’s instructions and was used for rabbit immunization ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed using Ni-NTA agarose (Qiagen). For FRET experiments ...
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bioRxiv - Immunology 2020Quote: ... His-tagged Fabs were loaded onto Ni-NTA superflow resin (Qiagen) preequilibrated with Buffer A (10 mM Tris ...
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bioRxiv - Biophysics 2022Quote: The following anti-His fluorophore-conjugated antibodies were purchased from Qiagen and used in UV-Bind assays for His-tagged proteins ...
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bioRxiv - Molecular Biology 2022Quote: The His-tagged protein was purified by Ni-NTA agarose (Qiagen). Eluted proteins from beads were then applied to the NGC chromatography system (Bio–Rad) ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... His-tagged Usp2-cc was bound to Ni-NTA Agarose (QIAGEN) equilibrated in buffer F (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... coli using the QIAprep Spin Miniprep kit (Qiagen, #27104). Genomic DNA (gDNA ...
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bioRxiv - Microbiology 2020Quote: ... coli by affinity chromatography using Ni-NTA agarose (Qiagen) according to the manufacturer’s instructions (Ruc-His6 was purified under urea denaturing conditions) ...
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bioRxiv - Microbiology 2020Quote: ... coli using the QIAprep Miniprep kit (QIAGEN, Germantown, MD) and confirmed by PCR to contain the construct again using the CjNC110F2 and CjNC110R2 primers.
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bioRxiv - Microbiology 2022Quote: ... coli via QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) prior to restriction enzyme digestion ...
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bioRxiv - Microbiology 2021Quote: ... coli with the Qiagen mini plasmid preparation kit (Qiagen). Pneumococcal DNA was isolated by phenol/chloroform extraction and ethanol precipitation from an exponentially growing culture in ThyB media (30 mg/ml Todd-Hewitt Broth powder and 0.2 mg/ml yeast) ...
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bioRxiv - Microbiology 2019Quote: ... coli using the QIAprep® Spin Miniprep Kit (Qiagen) and the DNA was eluted with nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... coli cultures with AllPrep PowerFecal DNA/RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified on Ni-NTA agarose beads (Qiagen) using standard protocols ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or from fresh or silica gel dried leaves using the Plant DNeasy Extraction Kit (Qiagen; Table S1). Libraries from herbarium samples were prepared with 22–157 ng of gDNA using Illumina TruSeq Nano DNA LT Sample Prep kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... V3 and V6 (Table S1) were sequenced by extracting the DNA using DNeasy Blood & Tissue Kit (Qiagen) from overnight cultures ...
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bioRxiv - Immunology 2022Quote: ... Nanobodies with 6xHis tag were captured by Ni-NTA Agaose (QIAGEN) and eluted by 500 nM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... SUMO-tag and protease were removed using Ni-NTA beads (Qiagen) and the protein of interest dialyzed in a sucrose buffer (250 mM sucrose ...
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bioRxiv - Genetics 2022Quote: ... primary erythroblasts or BFU-E using the RNeasy micro kit (QIAGEN). Reverse transcription of mRNA was performed using the SuperScriptIII First-Strand Synthesis System for RT-PCR (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2022Quote: ... His- tagged human UHRF1 was purified using Ni-NTA sepharose resin (Qiagen). Recombinant E1 (His-UBE1) ...
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bioRxiv - Biochemistry 2021Quote: ... Electroblotted membranes were incubated with penta-His antibody (Qiagen, Valencia, CA, USA). The antigen–antibody complex was visualized with alkaline phosphatase-linked to anti-rabbit IgG followed by staining with 5-bromo-4-chloroindol-2-yl phosphate and nitro blue tetrazolium [36].
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bioRxiv - Biophysics 2020Quote: ... His-tagged Chd1 was purified by Ni-NTA agarose affinity chromatography (Qiagen) and treated with homemade TEV protease to remove the tag ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...