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Citations for Qiagen :
301 - 350 of 615 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Western blot analysis was performed using the Penta His HRP conjugate kit (Qiagen). To check for equal loading ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP was first isolated using Ni-NTA beads (Qiagen, Valencia, CA) using a buffer with an imidazole gradient (20 mM Bis-Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged TEV protease was removed by incubation with Ni-NTA (Qiagen) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... a penta-His HRP conjugate was used (1:4000, QIAGEN GmbH, Hilden, Germany). The detection of GFP was performed with the primary antibody anti-GFP (1:10000 ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: Assay mixes consisted of 25 nM ALEXA488-conjugated penta-His antibody (Qiagen # 35310), 50 μM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged fusion proteins were purified with a Ni-NTA column (Qiagen), and the elution fraction was incubated with TEV protease for 15-18 h at 4°C to cleave the N-terminal His-tag ...
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Target protein was recovered and purified with a His-Trap affinity column (QIAGEN) according to manufacturers’ protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Preparation of his-tagged recombinant proteins was performed according to manufacturer instructions (Qiagen). Preparation of GST-tagged recombinant proteins was performed according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Neuroscience 2024Quote: ... The His-tagged nanobodies were purified by using Ni-NTA purification column (Qiagen) followed by desalting step using disposable PD-10 desalting columns (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... His-tagged SLFN11 (residues 349-901) was purified by Ni-NTA beads (Qiagen). All purified GST- or His-tagged proteins were dialyzed against a buffer containing 50 mM Tris-HCl and 150 nM NaCl and validated by Coomassie blue-stained SDSLJPAGE.
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bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal antibody that recognizes the strep-tag epitope was purchased from Qiagen. Recombinant NifB expressed in E ...
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bioRxiv - Microbiology 2023Quote: The three cell mock communities (even, staggered, and spike-in) were diluted with Buffer AVE (Qiagen, Hilden, Germany) and split into eight replicates per mock and dilution for subsequent DNA extraction ...
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bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
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bioRxiv - Neuroscience 2021Quote: ... coli DH5α and purified with a EndoFree Plasmid Maxi Kit (QIAGEN). The plasmids were then digested with AflIII and XbaI (both NEW ENGLAND BIOLABS ...
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bioRxiv - Cell Biology 2021Quote: ... coli and plasmid DNA was isolated by Plasmid Maxi-Prep (Qiagen). MCF10DCIS.com-GFP cells were cultured overnight to approximately 80% confluence ...
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bioRxiv - Molecular Biology 2021Quote: ... coli DH5α-pir and purified with QIAprep spin miniprep kit (Qiagen) according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... coli DH5α and purified using QIAprep spin mini-prep kit (Qiagen). All cDNA constructs were verified by DNA sequencing and services ...
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bioRxiv - Microbiology 2020Quote: ... coli strains was purified using the QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... coli cells using the QIAprep spin miniprep kit (Qiagen, Hilden, Germany). Electrocompetent C ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli NEB5α and isolated by miniprep according to the manufacturers (Qiagen) protocol ...
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bioRxiv - Genetics 2020Quote: ... coli cultures was performed with the QIAprep Spin Miniprep kit (Qiagen) and was combined with FastPrep (MP Biomedicals ...
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bioRxiv - Genomics 2020Quote: ... coli culture following infection using the RNeasy Mini kit (Qiagen: #74106) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... coli (DH5α) and afterwards isolated with a mini-prep kit (Qiagen) according to the manufacturer’s instructions as described below ...
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bioRxiv - Microbiology 2020Quote: ... coli M15 containing plasmid pREP4 to produce the LacI repressor (Qiagen). These strains were grown in 1L LB broth at 37°C with aeration until the OD 600 nm was 0.6-1.0 ...
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bioRxiv - Plant Biology 2023Quote: ... coli cells using a miniprep kit from Qiagen (Germantown, MD, USA) following the recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... coli DH5α and isolated using Qiagen QIAprep Spin Miniprep Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted for the ancestral strain (AN) and a single clonal isolate from each evolved population (S1-S10) using QIAamp DNA Blood Midi Kit (Qiagen) following the manufacturer’s recommendations from 50 mL culture incubated at 17 °C for 5 days in 75 cm2 flasks ...
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bioRxiv - Molecular Biology 2020Quote: ... and oligos P5 and PE (see Table S1) size selected on a agarose gel and extracted using the MINelute extraction kit (QIAGEN).
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bioRxiv - Biophysics 2019Quote: ... Total RNA was isolated from all generated cell lines (Table S1) at day 9 of differentiation using either the RNeasy Mini kit or the AllPrep DNA/RNA Mini kit (Qiagen). ATAC-seq in four cell lines (KO ...
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bioRxiv - Evolutionary Biology 2021Quote: ... two subadult flat males and six (four Gg and two gg genotypes) adult females (Table S1) using the RNeasy Plus Universal Mini kit (Qiagen). Before extraction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from the 730 samples (370 A. millepora and 360 Pocillopora; Tab. S1) were extracted using the DNeasy 96 Tissue kit (Qiagen) following manufacturer instructions.
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from PBMCs obtained from diverse blood withdrawals (Supplementary Fig. S1) and MLung-derived TILs with DNeasy Blood & Tissue Kit (Qiagen). Whole exome sequencing and single nucleotide variant analyses from MInt were available from our previous publication and were afterwards obtained for MLung ...
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bioRxiv - Molecular Biology 2022Quote: ... We designed species specific MALAT1 tiling primers for human and green monkey (Table S1) and performed multiplex PCRs following Qiagen Multiplex PCR protocol (Qiagen). cDNA was divided equally between primer sets ...
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bioRxiv - Molecular Biology 2022Quote: ... SiCBL4 and SiCBL7 promoters were respectively amplified using biotin-labeled primers (Table S1) synthesized by Sangon Biotech (Shanghai China) and purified using a PCR purification kit (Qiagen). EMSA was conducted using a LightShift Chemilumniescent EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: Total genomic DNA was extracted from tissue samples (Supplementary Table S1) preserved in 95% ethanol using the QIAampDNA extraction kit (Qiagen). Polymerase chain reaction (PCR ...
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bioRxiv - Genomics 2023Quote: ... microbial DNAs from stool samples of three individuals (S1, S2, and S3) were extracted using the QIAamp DNA stool mini kit (Qiagen) and size-selected using a BluePippin instrument targeting the size range of 10-50 Kb according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... 200nM siRNA/well were used for transfection using 5µl/well of Hi-Perfect (Qiagen) following manufacturer’s recommendation.
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bioRxiv - Biochemistry 2020Quote: ... but with an anti-His antibody (Qiagen, #34660 at a dilution of 1:5000) as primary ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... His-βC1 fusion proteins were purified using Ni-nitrilotriacetate (Ni-NTA) agarose (Qiagen, 30210) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... N-terminal His-tagged proteins were purified using a QIAexpress protein purification system (Qiagen), as previously described47.
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bioRxiv - Evolutionary Biology 2021Quote: ... His-tagged proteins from the supernatant were incubated overnight with Ni-NTA agarose (Qiagen) at 4°C ...