Labshake search
Citations for Qiagen :
351 - 400 of 5919 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... One-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) with 7.8 μl purified viral RNA or specified quantities (2.5 ng or 100 ng ...
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bioRxiv - Cell Biology 2020Quote: ... was used to extract RNA and the OneStep RT-PCR Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Genomics 2020Quote: ... while the cDNA amplicons were made with OneStep RT-PCR kit (QIAGEN). Manufacturer protocols were followed for all experiments ...
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bioRxiv - Cell Biology 2019Quote: ... in a single step using the Qiagen OneStep RT-PCR Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Amplifications were done using the OneStep RT-PCR kit (QIAgen, California, USA) with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system under these cycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) and BIO-RAD T100 Thermal Cycler ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen) using the 1x Takyon SYBR Master Mix (Eurogentec ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was performed using the QuantiFast SYBR Green PCR kit (Qiagen). The PCR reaction was conducted on a Corbett Rotor-Gene 6000 (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and the S gene was amplified using OneStep RT-PCR Kit (Qiagen). The mutations were identified by Sanger sequencing (GENEWIZ) ...
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bioRxiv - Immunology 2022Quote: ... and the S gene was amplified using OneStep RT-PCR Kit (Qiagen). The mutations were identified by Sanger sequencing (GENEWIZ) ...
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bioRxiv - Microbiology 2022Quote: ... All assays were adapted to the One-step RT-PCR Kit (Qiagen) reaction chemistry ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative RT-PCR was performed in triplicate using SYBR green mix (Qiagen) on an ABI 7500 machine (Applied Bio Systems) ...
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bioRxiv - Genetics 2019Quote: ... RT-qPCR was performed with QuantiTect SYBR Green PCR Kit (QIAGEN, Germany) according to the manufacturer’s instruction by CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: RNA was quantified using the QuantiFast SYBR Green RT-PCR Kit (Qiagen). Primers used were for Esr1 (QuantiTect Primer Assays ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative RT-PCR was performed using the SYBR Green detection system (Qiagen) according to the manufacturer’s instructions in an iCycler (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Immunology 2022Quote: ... and the S gene was amplified using OneStep RT-PCR Kit (Qiagen). The mutations were identified by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2022Quote: ... then the template was amplified using the OneStep RT-PCR Kit (Qiagen) with the EILV-specific primers 5’-CGA CGA TGA CCG GAG AAG AG -3’ and reverse primer 5’-AAG ACT CGG TCT GCC TGC −3’ ...
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bioRxiv - Neuroscience 2023Quote: ... gcy-8 mRNA was detected using a OneStep RT-PCR kit (QIAGEN) from 3 ng of template RNA ...
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bioRxiv - Physiology 2023Quote: ... RT-qPCR was then performed with QuantiTect SYBR Green PCR Kit (Qiagen) on a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... RT PCR was carried out in a Rotor-gene Q2 PLEX (QIAGEN), using SensiMix SYBR master mix (Bioline) ...
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bioRxiv - Microbiology 2023Quote: ... A nested RT-PCR (QIAGEN hot start Taq master kit 1000 units) was then performed from 2 µL of previous amplicon for 15 min at 95°C then 35 cycles of amplification (30 sec at 94°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The ligated products were amplified by PCR with Illumina primer pairs and purified with PCR purification kit (QIAGEN). 5C libraries were sequenced with the 90 bp pair-end mode by Hi-seq 2500 platform of Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time reverse transcription PCR (qRT-PCR) was carried out with the QuantiNova SYBR Green RT-PCR kit (Qiagen, Manchester USA) using a LightCycler® 480 Instrument II (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using the Custom RT² Profiler PCR Array according to the manufacturer’s instructions (Qiagen), and detection was performed using the ABI Prism® 7500 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... RNA samples were subjected to KSHV real-time PCR array using the QuantiTect Probe RT-PCR Kit (Qiagen). The copy number of each transcripts was normalized to that of GAPDH transcripts and the ratio of treated versus untreated cells was calculated.
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bioRxiv - Neuroscience 2021Quote: ... cDNA was processed for real time q-PCR using SYBR-green (QuantiFast SYBR Green RT-PCR Kit, Qiagen) and determination of ΔΔCT-values was done on the CFX Maestro Software (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was reverse transcribed and quantified by real-time PCR using the OneStep RT-PCR kit (Qiagen) as described previously(40 ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed using QIAGEN’s RT2 Profiler™ PCR Array Human Cell Death PathwayFinder (QIAGEN, Hilden, Germany). RT-PCR data was analyzed and visualized for pathway analysis by GeneSpring GX 14.9 (Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: Isolated miRNA content was quantified by real time quantitative PCR (RT-qPCR) using the miScript PCR Kit (Qiagen) with a CFX Connect Real-Time system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: Real-Time Quantitative PCR (RT-qPCR) was performed with the Rotor-Gene SYBR® Green PCR Kit (Qiagen), on a Rotor Gene-Q(R ...
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bioRxiv - Genomics 2021Quote: ... Synthetic cel-miR-39 mimic (miScript miRNA Mimics, QIAGEN) was spiked to each RNA sample at a ratio of 11 fmol per ug of total RNA and used as an exogenous control for normalization ...
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bioRxiv - Cell Biology 2022Quote: ... Syn-cel-miR-39 spike in synthetic RNA (Qiagen) was added to monitor extraction efficiency ...
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bioRxiv - Cancer Biology 2020Quote: ... at a final concentration of 50 nM and confirmed by RT-qPCR with the miRCURY LNA™ Universal RT microRNA PCR system (Qiagen) and a StepOnePlus thermocycler (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... A one-step real-time quantitative polymerase chain reaction (RT-qPCR) was performed using a QuantiFast SYBR Green RT-PCR one-step kit on a Rotorgene 3000Q thermocycler (Qiagen, UK). Each reaction was setup as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR and sequencing primers were designed using the PyroMark Assay Design software (Qiagen). All successfully amplified PCR products were analysed on a PyroMark Q24 (Qiagen) ...
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bioRxiv - Systems Biology 2019Quote: ... with custom miRCURY LNA PCR primers (Qiagen cat. no. 339306, Supplementary Table 7). Each 10 µL reaction volume contained 5 µL 2x miRCURY SYBR Green Master Mix ...
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bioRxiv - Biophysics 2020Quote: ... Real-Time PCR was performed with specific primers purchased from Qiagen (Tables 1,2) and Power SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real time PCR was performed using SYBR Green with QuantiTect Primers (Qiagen) and using ppia gene as a reference gene ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR and sequencing primers were designed by Pyromark Assay Design Software 2.0 (Qiagen) and available upon request ...
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bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
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bioRxiv - Cancer Biology 2021Quote: ... For evaluation of correlation with ALDH1A1 and ALDH1A3 the RT2 profiler PCR Array gene sets (Qiagen) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... All experiments were done with Qiagen OneStep RT-PCR reagents (Qiagen, Hilden, Germany) on a MIC thermocycler (BMS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RT-qPCR was performed in SYBR Green PCR Master Mix (QIAGEN, California, USA) on CFX-96 system ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed using the miScript SYBR Green PCR Kit (QIAGEN, 218073) on a step-one plus PCR machine (Applied Biosystems) ...
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bioRxiv - Biochemistry 2020Quote: ... and QIAGEN® OneStep RT-PCR Kit were purchased from QIAGEN (Frederick, MD). RiboMAX™ Large Scale RNA Production Systems-T7 was purchased from Sigma-Aldrich (St ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed using QuantiTect SYBR® Green PCR Kit (#204145, QIAGEN) on Agilent MP3005P thermocycler ...
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bioRxiv - Microbiology 2021Quote: ... the QIAcuity One-Step Viral RT-PCR Kit (Cat No. 1123145, Qiagen, Germany) and the 24-well 26K Nanoplates (Cat No ...