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Citations for Qiagen :
501 - 550 of 5919 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward: 5’-GGCACCGAGAAAGACAAAGT-3’; reverse: 5’-CTCTGGAAATGGGCTTCAAA-3’) and PCR products were purified using QIAquick PCR purification kit (QIAGEN, Cat #28104) before Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... We obtained single amplified products for12 out of the 14 PCR primer pairs and these amplified products were purified using the QIAquick PCR Purification Kit (28104, Qiagen, Germantown, MD) before sequencing ...
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bioRxiv - Biochemistry 2020Quote: Reverse transcription reactions containing 2μl of purified RNA and 2μl of 10mM HNqPCRrev1 primer in a final volume of 20μl were performed using the Qiagen Sensiscript RT kit (Qiagen, catalog # 205211) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen, Courtaboeuf). Experiments were performed with at least triplicates for each data point ...
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bioRxiv - Cancer Biology 2021Quote: RT-PCR was performed using the SYBR green master mix (RealQ plus 2X, Qiagen-Germany). Amplification was done by Exicycler96 (Bioneer-S ...
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bioRxiv - Microbiology 2020Quote: ... vIL-6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using QuantiTect Multiplex RT-PCR Kits (Qiagen) as described previously (37 ...
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bioRxiv - Cancer Biology 2021Quote: ... and selected genes were amplified by Q-RT-PCR with SYBR green (Qiagen, Valencia, CA) on the FAST Model 7900HT (Applied Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand synthesis and RT-PCR were performed using QuantiTect™ Reverse Transcription Kit (Qiagen) and Rotor-Gene™ SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... EGR1 transcripts were converted into cDNA and quantified using a OneStep RT-PCR kit (Qiagen) and a pair of primers (Table S1) ...
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bioRxiv - Physiology 2020Quote: ... cDNA was prepared by reverse transcription using the Quantitect RT-PCR kit (205313, Qiagen, DEU). cDNAs were amplified using assay-on-demand kits (Gpt ...
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bioRxiv - Cell Biology 2020Quote: ... For the RT² Profiler™ PCR Array Human Epithelial to Mesenchymal Transition kit (EMT) (Qiagen), RNA integrity of all the samples was tested by using the Agilent RNA 6000 Nano kit (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The harvested contents were subjected to RT-PCR using OneStep Kit (210212, Qiagen, Hilden, Germany). Forward (F ...
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bioRxiv - Physiology 2023Quote: ... Quantitative real-time RT-PCR was performed in a Rotor-Gene Q (Qiagen, Hilden, Germany) using the 5x QPCR Mix EvaGreen (No Rox ...
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bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
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bioRxiv - Physiology 2021Quote: ... reaction tubes were transferred to a Rotor-Gene Q PCR thermal cycler for product amplification using a one-step protocol (QuantiFast SYBR® Green RT-PCR Kit, Qiagen, UK). The amplification protocol was as follows ...
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bioRxiv - Neuroscience 2020Quote: ... Those standards were generated by mixing commercially available unmethylated and hypermethylated DNA standards (EpiTect PCR Control DNA Set, Qiagen) resulting in 10 ng of total bisulfite-converted DNA ...
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bioRxiv - Immunology 2019Quote: ... RNA collected for real time PCR experiments was further purified using column DNA digestion (RNase-Free DNase set, Qiagen). cDNA was prepared using iScript cDNA-synthesis kit (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... the reactions were pooled in sets of four and purified on one column each (MinElute PCR purification kit, Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... using miScript Primer Assays for miRNAs and the using the QuantiTect SYBR Green PCR kit (Qiagen) for mRNA in a Stratagene Mx3005P qPCR machine (Agilent) ...
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bioRxiv - Genetics 2020Quote: ... Primer design was optimised to work with the Type-it Microsatellite PCR kit (QIAGEN, Hilden, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... with primers pBEST_LinL_F and pBEST_LinL_R (Table S1) and subsequent purification using QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
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bioRxiv - Immunology 2020Quote: Primer pairs were used in this study together with a QuantiFast SYBR green PCR kit (QIAGEN). The sequences of primer pairs used in this study are listed in Supplementary Table 3 ...
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bioRxiv - Immunology 2023Quote: ... and the purified pools were barcoded with Illumina indexing primers using a Multiplex PCR Kit (QIAGEN). All indexed pools for each sample were then pooled during column purification ...
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bioRxiv - Physiology 2019Quote: ... primers obtained from Qiagen (Quantitect Primer Assay, QT00101962) were used with an expected band length of 96bp ...
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bioRxiv - Cell Biology 2022Quote: The reverse primer was miScript Universal Primer (Qiagen).
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bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (Qiagen) was used to remove DNA ...
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bioRxiv - Immunology 2022Quote: ... RNase-free DNase Set (Qiagen) was used to remove potential DNA contamination ...
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bioRxiv - Genomics 2019Quote: ... and DNase set (79254; Qiagen), followed by delusion with 10 mL of RNase-free water ...
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bioRxiv - Neuroscience 2021Quote: ... dNTP set was from Qiagen. Plasmid purification kits were from ROBOKLON.
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bioRxiv - Plant Biology 2021Quote: ... RNase-Free DNase Set (Qiagen) was used according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (QIAGEN) was used to remove all genomic DNA ...
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bioRxiv - Cell Biology 2024Quote: ... RNase-Free DNase Set (Qiagen) was used to remove genomic DNA contaminants ...
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bioRxiv - Bioengineering 2020Quote: ... and linearly amplified with RT2 miR First Strand Kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The scramble-miR miRCURY LNA Detection probe (5’-GTGTAACACGTCTATACGCCCA-3’, YD00699004, QIAGEN) was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... with 4 × 108 copies of cel-miR-39 miRNA mimic (Qiagen 339390) spiked into to the sample after addition of lysis buffer.
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bioRxiv - Neuroscience 2024Quote: ... mice received a 0.5μM dose of a miR-505-5p mimic (Qiagen) or negative control combined with HiPerfect transfection reagent (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Primers (Qiagen): Gapdh QT00199633 ...
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bioRxiv - Molecular Biology 2021Quote: ... The blood samples from the 2020 FMD outbreak were analysed using a one-step PCR assay using the QIAGEN One-step RT-PCR Kit (Qiagen GmbH, Hilden, Germany) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed on cDNA using a Rotor-Q Real-Time PCR Cycler from Qiagen using gene-specific primers and normalized to the ACTIN gene (listed in Supplemental Table 1).
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bioRxiv - Cell Biology 2022Quote: ... Real time RT-PCR was performed using SYBR Green Fluor qPCR Mastermix (Qiagen, Limburg, The Netherlands) on a CFX Connect (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR of cDNA samples was performed using 2× Rotor-Gene SYBR Green PCR Mastermix (Qiagen) and Rotor-Gene Q real-time PCR cycler (Qiagen).
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bioRxiv - Pathology 2019Quote: ... Quantitative RT-PCR gene expression analysis was performed on duplicate samples using fast SYBR green (Qiagen) technology using the ViiA 7 Real-Time PCR System (Applied Biosystem) ...
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bioRxiv - Pathology 2021Quote: ... a RT-qPCR was performed as quality control step using miRCURY LNA miRNA PCR Assays (Qiagen) to test UniSp6 (Cat No ...
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bioRxiv - Microbiology 2020Quote: All qPCR and RT-qPCR-reactions were done using the QuantiTect Multiplex PCR NoROX Kit (Qiagen) or QuantiTect Probe RT-PCR Kit (Qiagen ...
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bioRxiv - Systems Biology 2021Quote: ... the presence of RVFV RNA was assessed using the QuantiNova® RT-PCR probe kit (QIAGEN). Samples were tested in duplicate using 5 μl of RNA template per well.
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed with a 6plex real-time PCR system (Qiagen, Rotor Gen Q, Germany) using the RealQ Plus 2x Master Mix Green kit ...
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bioRxiv - Immunology 2024Quote: ... Cells were sorted directly into 12.5 μl (per well) of OneStep RT-PCR Buffer mix (QIAGEN) in a 96-well PCR plate (one cell/well).
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bioRxiv - Microbiology 2022Quote: ... We performed purification between the two PCRs to reduce contaminants or carry-over of primer dimers using 96-well PCR purification plates (Qiagen Inc., Germantown, MD, USA). The final libraries were quantified with a Qubit Fluorometer (ThermoFischer ...