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Citations for Qiagen :
351 - 384 of 384 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the plates were imaged to verify expression of the fluorescent reporter before column-based isolation of RNA (Qiagen, RNeasy Mini Kit) with on-column DNA digestion ...
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bioRxiv - Cell Biology 2024Quote: APOB expression determined by first isolating total RNA from Hep3B cells grown in 6-well plates using the RNeasy Mini Kit (Qiagen, #74104). Real-time qPCR was performed using the TaqManTM RNA-to-CtTM 1-Step Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Single viable tumor cells were selected by positive staining for Calcein AM as well as negative staining for TO-PRO3 and sorted into pre chilled (4 °C) 96 well plates containing TCL buffer (Qiagen, 1031576) + 1% β-mercaptoethanol (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... two coverslips from a 24-well plate of neurons were combined into 350 µL RLT buffer from the RNeasy Micro Plus kit (Qiagen, #74134), supplemented with 1% beta-mercaptoethanol (PanReac AppliChem ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from 14-day-old thalli grown on the agar plates using RNeasy Plant mini Kits (QIAGEN, Netherlands). Library preparation and sequencing were conducted by Novogene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The freshly isolated chondrocytes were plated at the density of 1.2 x 105/cm2 and reverse transfected on the PCR array plate (RT² Profiler™ PCR Array Mouse Extracellular Matrix & Adhesion Molecules, Qiagen). On the next day ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was later harvested from the plates by briefly thawing prior to using extraction by RNeasy Mini kit (Qiagen Cat# 74106) and QIAshredder (Qiagen Cat#79654) ...
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bioRxiv - Immunology 2024Quote: Cells were seeded (3 x 105) in 6-well plates and lysed using RLT buffer for RNA isolation (RNeasy Mini kit, Qiagen, #74106). Each sample was performed in triplicate ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from the samples using the QIAamp® DNA Micro kit or the DNeasy Blood & Tissue kit (spin column or plate extraction kits, Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cells were allowed to reach 80% confluency in a 6-well plate before total RNA was extracted using RNeasy kit (QIAGEN #74106) as per manufacturer protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One million cells per well were seeded in 6 well plates in 2 mL growth medium the day before transfection and transfected with PolyFect (Qiagen, Düsseldorf, DE) using the manufacturers protocol (changing the medium to growth medium without hygromycin B and puromycin prior to transfection) ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Biochemistry 2020Quote: Cells seeded in 6 well plates were grown in 5% FBS to a confluency of 70% before RNA extraction using the RNEasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
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bioRxiv - Microbiology 2021Quote: ... Liquid cultures or swabs from agar plates from selected bacterial strains (Supplementary Data 3) were used to extract DNA using the QiAmp Micro DNA kit (Qiagen, Hilden, Germany). The extracted DNA was treated with RNase ...
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bioRxiv - Cell Biology 2020Quote: ... All HA positive clones were amplified in a 6 well plate and genomic DNA isolated using Qiagen DNAeasy blood and tissue kit as per manufactures instructions (Qiagen, Germantown, MD) (Cat #69504) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Cell Biology 2023Quote: ... 0.4×106 S2R+ cells were seeded in 6-well plates and were transfected with 1 μg of each pMK33-ARRDC-GFP construct using Effectene transfection reagent (#301425, Qiagen, Venlo, Netherlands). pMK33 plasmid is a copper-induced protein expression vector ...
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bioRxiv - Developmental Biology 2024Quote: ... collected into 96-well plates (on ice) prior to the addition of 10 μL of RLT plus lysis buffer (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% v/v ethanol ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... First strand cDNA was synthesized from 0.5 mg of total RNA using QIAGEN RT2 First Strand Kits and transcripts for mouse cytokines and chemokines were quantified using qPCR microarrays comprising 96-well plates precoated with primers (QIAGEN PAMM-150Z). Each 96-well plate also contained primers for 5 housekeeping genes as well as positive and negative controls ...
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bioRxiv - Microbiology 2024Quote: ... 1×105 cells were seeded onto type I collagen-coated 24-well plates and transfected with 200 ng of viral genome using Attractene Transfection Reagent (Qiagen, Hilden, Germany). One day post-transfection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... individual colonies on the LB agar plate were readied for miniprep by following the standard protocol described by QIAprep Spin Miniprep Kit (Cat# 27104, Qiagen, Hilden, Germany) Plasmid DNA samples were sequenced following standard sequencing sample preparation and submission described by Eton Bioscience (San Diego ...
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bioRxiv - Cell Biology 2024Quote: ... S2 cells were transfected with 0.5 µg of DNA in a 12-well plate using the Effectene transfection kit (Qiagen, Cat. #/ID: 301425) and plated 48 hours after transfection on acid-washed No ...
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bioRxiv - Genomics 2021Quote: ... PI-negative fraction was sorted in each well of a 96 well plate containing 3 μL of PBS (Qiagen, REPLI-g SC Kit) with 0.2% Tween 20 ...
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bioRxiv - Cell Biology 2021Quote: Drosophila S2R+ cells were transfected with 0.5 µg DNA (pAC-Gal4+pUASp-GEM, or pAC-LAMP1-GFP 77) in 12-well plate using Effecetene transfection kit (Qiagen, Cat. # / ID: 301425) and then were plated 48 hours after transfection on concanavalin A-coated glass coverslips 78 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated at a density of ∼100,000 cells/well in a 24 well plate and transfected the following day with 500 ng of midi-prepped endotoxin-free plasmid DNA (Qiagen, Germantown, MD, USA) using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Microbiology 2022Quote: ... and mutants recovered from selected LB plates after collection from the ligature (oral) and fecal (gut) samples (output) using the DNeasy Blood & Tissue Kit (Qiagen Sciences, Germantown, MD) [36] ...
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bioRxiv - Microbiology 2024Quote: ... 200 nl in MRC 3-drop 96-well plates using a Dragonfly robot (SPT Labtech) with a range of commercially available screens (Molecular Dimensions, Hampton Research, Qiagen-Nextal). Thick needle-like crystals were observed in the drops containing the solution of 1.6 M LiCl ...
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bioRxiv - Genomics 2024Quote: ... bacteria were collected in a 1ml PBS rinse of the plate for DNA extraction with the Qiagen DNeasy Blood and Tissue Kit (Qiagen# 69504, MD, USA).
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bioRxiv - Biochemistry 2022Quote: ... and FdxE−CYP143 (200–250 µM) were crystallized in 96-well plate using a sitting-drop method with commercially available kits from Qiagen (NeXtal Classics II screen) and Molecular Dimensions (Structure screens 1 and 2 ...
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bioRxiv - Microbiology 2022Quote: ... We performed purification between the two PCRs to reduce contaminants or carry-over of primer dimers using 96-well PCR purification plates (Qiagen Inc., Germantown, MD, USA). The final libraries were quantified with a Qubit Fluorometer (ThermoFischer ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were picked onto fresh plates and genomic DNA extraction was carried out using the QIAamp® DNA Mini Kit (QIAGEN; cat. number: 51306) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... After 72 h cells were passaged onto new 6-well plates and culturing continued for an additional 72 h after which cells were harvested in Buffer RLT (Qiagen; for RNA-sequencing, RT-qPCR) or used for cell culture experiments.