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Citations for Qiagen :
201 - 250 of 384 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: Genomic DNA was isolated from Streptomyces plates or liquid cultures using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germany). The bead beating was performed using a TissueLyser LT (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Screening plates were set up by a sitting-drop vapor diffusion method using JCSG Core I-IV (Qiagen), PEG Suite I-II (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: RNA from human cell cultures was extracted from 6 well plates using RNeasy Plus micro kit (Qiagen, 74034). The plates were washed once with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Colonies were selected on LB+Amp agar plates and plasmid was isolated for downstream use (Qiagen Midiprep Kit). pUC57-HaloTag-ACTB-18xenv8-FL and PB510B-1 (System Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 10,000 cells per sample were sorted into a 96 well plate containing 50 μl RLT plus Lysis Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol and frozen at −80 °C immediately ...
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bioRxiv - Genomics 2021Quote: ... in a 15 ul reaction volume in 96-well plates or with the Repli-g Single Cell kit (Qiagen) in a 10 ul reaction volume in 384-well plates ...
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bioRxiv - Biochemistry 2020Quote: CYP124–SQ109 was crystallized by a sitting drop approach in 96-well crystallization plates with commercially available kits (Qiagen) at 20 °C with 1:1 protein/mother liquor ratio with the ligand concentration of 100 μM ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110 μL of Buffer AE (QIAGEN) preheated to 56°C ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 12 well plates at a dilution of 1.5×106 and were transfected with the indicated constructs using Effectene (Qiagen) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... Two hundred fifty microliters of each sample were transferred into 96-well PowerBead Plates (Qiagen, 27500-4-EP-BP). Seven hundred fifty microliters of RLT buffer from an AllPrep DNA/RNA 96 Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at 800,000 in 60 mm plates and 24 h later RNA was isolated (Qiagen RNeasy Kit). 1 ug of RNA was reverse transcribed (Applied Biosystem) ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from hESCs or NPCs grown in a 6-well plate (9.5 cm2) using the RNeasy minikit (Qiagen). A DNase 1 digestion step was performed during RNA extraction to degrade remaining genomic DNA ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at 800,000 in 60 mm plates and 24 h later RNA was isolated (Qiagen RNeasy Kit). 1 μg of RNA was reverse transcribed (Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit) ...
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bioRxiv - Immunology 2022Quote: ... Single cells from three to four mice per each condition were index-sorted directly into 96-well plates containing 5μl of TCL buffer (Qiagen) supplemented with 1%β-mercaptoethanol using a BD FACS Aria II instrument ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was isolated from cells in 96-well plates using the Qiagen FastLane Cell Probe Kit (QIAGEN, 216413), according to the manufacturer’s instructions to a final volume of 40 μL per well ...
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bioRxiv - Genetics 2020Quote: ... Sample libraries were purified using a plate from a MinElute 96 UF PCR Purification Kit (QIAGEN Inc., Hilden, Germany), vacuum manifold ...
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... and RT2 Profiler PCR Array Mouse WNT Signaling Pathway ABI 7900HT Standard Block plates (Qiagen; Cat. No. PAMM-043ZA) were used for qPCR.
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bioRxiv - Molecular Biology 2023Quote: ... 10µL of diluted nuclei were distributed to all wells of a 96-well plate and 1µL EB buffer (Qiagen) 0.5µL Proteinase K (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... and sorted into wells of a 96-well plate (185 nuclei/well) containing reverse cross-linking buffer (11µL EB buffer (Qiagen) 0.5µL Proteinase K (Roche) ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were prepared in duplicates in a standard 96-well PCR plate and transferred to a QIAcuity 8.5k 96-well Nanoplate (Qiagen) for partitioning using the Qiagen Standard Priming Profile ...
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bioRxiv - Immunology 2024Quote: ... or PMs were washed with PBS and then lysed in a culture plate using QIAzol Lysis Reagent (Qiagen 79306) as was described in our previous studies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial biomass from strains grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Physiology 2023Quote: ... RNA was pooled from 3-4 wells of an MEA plate and then extracted using the miRNeasy kit (Qiagen). RNA levels were measured using the nanoString nCounter® PlexSet™ (nanoString ...
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bioRxiv - Immunology 2023Quote: ... 100 μl His-tagged trimer diluted to 1.5 μg/ml in PBS (pH 7.4) or PBS only as a control was added per well to Ni- NTA 96-well plates (Qiagen) and incubated over night at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown in 6 well plates and total mRNA was isolated using the RNeasy Kit (Qiagen, 74-104) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Bacterial biomass from isolates grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Developmental Biology 2023Quote: Plates with the micropatterned coverslips were washed with PBS-/- and the cell colonies lysed in RLT buffer (QIAGEN 1015762). RNA extraction was performed using RNeasy mini kit (QIAGEN 74106 ...
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bioRxiv - Immunology 2023Quote: M1 and M2 macrophages (300,000/well, 96-well plate) were transfected on Day 7 with MALAT1 or control GapmeRs (Qiagen). MALAT1- or control GapmeR-transfected M1 and M2 Mφ were incubated with Texas Red-conjugated Ova and DQ-conjugated Ova (both 1 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110 μL of buffer AE (QIAGEN) preheated to 56 °C ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from ∼70% confluent HeLa cells grown in 6-wells plate using the RNeasy Micro Kit (Qiagen) with a DNase step according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from individual flies in 96 well plates using the Puregene Tissue Kit (Qiagen, Venlo, Netherlands). Each Illumina library was comprised 384 individual barcoded genomes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Colonies were washed from each agar plate with M9 and DNA was extracted using a DNeasy kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Single cells of Desulfovibrio or Methanococcus were sorted into wells of a 96-well plate containing 3 µl of PBS and Buffer D2 from Repli-G single cell kit (Qiagen) by using Influx flow cytometer (BD) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Infected cells were grown for three days before combining the plates of each replicate for extraction of RNA and DNA via the Qiagen AllPrep mini kit (Qiagen). We subsequently purified mRNA from the RNA using the Oligotex mRNA prep kit (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: Organoids from a single 48-well plate well were used for genomic DNA extraction with QIAamp Fast DNA Tissue Kit (51404, Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... with the exception of larger HSPC colonies which were extracted using the DNeasy 96 blood and tissue plate kit (Qiagen) and then diluted to 1-5ng ...
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bioRxiv - Systems Biology 2020Quote: ... followed by flow sorting with a Sony SH800Z (gating on calcein NVOC fluorescence levels) into individual wells of a 96-well plate containing 5 μL of Buffer RLT (Qiagen) and 1% β-mercaptoethanol.
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Systems Biology 2021Quote: ... Crystal screening was performed with a Gryphon robot (Art Robbins instruments, Sunnyvale, USA) using sitting-drop 96-well plates and Screening Suites (Qiagen). Optimization experiments were set up in 24-well hanging drop crystallization plates ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting lawns were scraped off the agar plates and the plasmid libraries were extracted using a QIAprep Spin Miniprep Kit (QIAGEN). The plasmid libraries were subsequently used for yeast transformation.
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from one 96-well microtiter plate of the 105-cell cryopreserved seawater inoculum cultures that exhibited growth using the DNEasy 96 Blood & Tissue Kit (Qiagen) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Our panel of 88 target genes was prepared on a 96-well plate format with housekeeping genes and controls (RT2 PCR array, Qiagen). Expression was quantified on an ABI7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
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bioRxiv - Genetics 2022Quote: Total RNA was purified from single wells of >85% confluent six-well plates using the RNeasy Plus Mini Kit (Qiagen), and an additional DNase I step was used to remove genomic DNA ...