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Citations for Qiagen :
251 - 300 of 384 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: The cells previously frozen in plates were thawed on ice and RT-PCR was performed using the OneStep RT-PCR kit (Qiagen) protocol without modification ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates (2 × 104 cells/well) and reverse-transfected with 100 ng/well of Cignal ISRE-Luc reporter (Qiagen) using Lipofectamine 3000 ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen) the next day ...
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bioRxiv - Immunology 2022Quote: ... The next day cells were washed off the plates and plasmids were purified using the HiSpeed Plasmid Maxi kit (Qiagen). The resulting replicate libraries were packaged into a lentiviral library using the packaging vectors psPAX2 and pMD2G and the LV-MAX lentiviral production system (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... A549-ORF7a and A549-ORF7b cells were seeded (3×10E5) in 6-well plates and lysed using RLT buffer for RNA isolation (RNeasy mini kit, Qiagen). Each sample was performed in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from sub-confluent plates using the RNeasy kit as directed by the manufacturer (Qiagen, Valencia, CA), as previously described (31) ...
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bioRxiv - Immunology 2022Quote: ... single cells were plated by serial dilution in 96-well plates for clonal selection and gDNA was extracted from the remaining pool using the DNeasy kit (Qiagen). The XBP1 locus was amplified by PCR and sequenced by Sanger sequencing using the primers listed in table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: ... we captured poly-adenylated mRNA from cell lysates using a 96-well plate with oligo(dT) grafted to the inner walls of each well (Qiagen). Next ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... single MR1-tetramer-binding cells from Peruvian participant 7-3 and blood bank donors 702A and 703A were sorted into 96-well plate coated with Vapor-Lock (Qiagen) containing Iscript cDNA synthesis mixture (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Immunology 2020Quote: ... 5 μl of amplified cDNA from each well of a 96-well plate were pooled and completed to 500 μl with PCR-grade H2O (Qiagen). Two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP ...
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bioRxiv - Immunology 2021Quote: ... and subsequent crystallization optimization was performed manually using hanging-drop vapor diffusion method with 15-well screw-cap crystallization plates (Qiagen). The H5.28-HA head complex was crystallized in 7-9% PEG 8000 ...
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bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... grown up at 20 °C were washed off from NGM plates using M9 solution and subjected to total RNA extraction using the RNeasy Mini Kit from Qiagen. Purification of poly-A containing RNA molecules ...
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bioRxiv - Cell Biology 2022Quote: ... animals were maintained at 20 °C and washed down from NGM plates using M9 solution and subjected to RNA extraction using TissueDisruptor and the RNeasy Mini Kit from Qiagen. RNA preparations were used for qRT-PCR or RNAseq ...
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bioRxiv - Developmental Biology 2022Quote: ... the animals were washed down from NGM plates using M9 solution and subjected to RNA extraction using the RNeasy Mini Kit from Qiagen. 1 μg total RNA from each sample was used for sequencing library construction ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was purified from single wells of >85% confluent six-well plates using the RNeasy Plus Mini Kit (Qiagen), and an additional DNase I step was used to remove genomic DNA ...
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bioRxiv - Genetics 2022Quote: ... were maintained at 20 °C and washed down from NGM plates using M9 solution and subjected to RNA extraction using the RNeasy Mini Kit from Qiagen. RNA-seq library preparation and data analysis were performed as previously described 46 ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Plant Biology 2022Quote: Leaf tissue samples were collected and freeze-dried in a deep-well plate and ground in a TissueLyser II (QIAGEN) for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Bioengineering 2022Quote: qRT-PCR of SARS-Cov-2 RNA was performed as previously described.59 RNA was extracted from human lung epithelial cells expressing human ACE2 (A549-hACE2) grown in 96-well plates by using the RNeasy 96 Kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson, Bioscience, Salt Lake City, UT, USA) using the QuantiTect SYBR Green (Qiagen) and LightCycler® 480 system (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... 10 Arabidopsis seedlings from the same MS plate were sampled together in 2 mL microtubes containing two 3 mm-diameter tungsten carbide beads (Qiagen), and flash frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... but not on BHIY/Thia plates were selected and DNA was isolated using a DNeasy blood and tissue kit (Qiagen) to investigate whether the intended chromosomal mutation was introduced ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Microbiology 2022Quote: DNA was purified for sequencing from cells infected in six well plates using a blood and cell culture DNA mini kit (Qiagen). Samples were sequenced at the Northwestern University Genomics Core Facility using NextGen Illumina HiSeq SR500 sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were selected from transformation plates and liquid bacterial cultures grown as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Neuroscience 2023Quote: ... and colonies yielding an ≈2.4 kB product were isolated from a duplicate patch plate and grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Molecular Biology 2023Quote: Primary monocytes were cultured in 24-well plates and then transfected with control non-targeting siRNA (Dharmacon) or siRNA targeting ERN1 (Dharmacon) using HiPerFect reagent (QIAGEN) following the instructions provided by the manufacturer ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from MU636 and set1 mutant strains growing in plates using the RNeasy Plant Mini Kit (Qiagen) and treated with DNase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: Total cellular RNA extraction from cells grown in a 24-well plate was performed using RNeasy Plus kit (Qiagen, # 74034) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells from single wells of a 6-well plate were pelleted and total RNA was extracted using the RNeasy MiniKit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the lawn of bacteria was scraped off the plates and plasmid DNA was prepared using the Plasmid Plus Maxi kit (Qiagen).
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bioRxiv - Genomics 2023Quote: ... The BD FACS Melody sorter device was used for sorting single cells into 96-well plates (FrameStar®, 4TI-0960/C) containing 2.5 μl RLT plus buffer (Qiagen). Plates were then spun down at 1,000g for 1 minute at 4°C ...
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bioRxiv - Biochemistry 2023Quote: A kinome-wide siRNA library that contained 4 individually arrayed siRNA sequences in 384-well plates was purchased from Qiagen. The library consisted of known kinases and associated proteins ...
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bioRxiv - Plant Biology 2023Quote: ... assays were performed with triplicates of cDNA aliquots in 96-wells reaction plates using Quantinova qPCR SYBR Green Master Mix (QIAGEN) according to the manufacturer’s manual ...
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bioRxiv - Genetics 2023Quote: Genomic DNA of subclones from a 12-well plate along with 2 million parental cells were extracted using DNeasy Blood & Tissue Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were scraped from each 96-position array selection plate and a pooled plasmid extraction was performed using Plasmid Plus Mini Kit (QIAGEN). The plasmid DNA was quantified and diluted to ∼ 1ng/μL ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from 2-week-old seedlings grown on an MS plate using RNeasy Plant Mini Kit (Qiagen) following the manufacturer’s instructions ...