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Citations for Qiagen :
3151 - 3200 of 3706 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a final volume of 20 μl containing Hot Start Taq (1 U, QIAGEN), 3.5 mM MgCl2 ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from stably transduced THP-1 cultures using the RNeasy Plus Mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Zoology 2019Quote: ... The lysate was then mixed with 450 µl 1 × TE buffer and 3750 µl PB buffer (Qiagen) and the entire volume of the mixture was centrifuged through a MinElute (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a Cyprus wheatear (O. cypriaca) (Table 1) using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany). Digestion was performed in 360 ul of buffer ATL ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was extracted from more than 1 × 107 cells using RNeasy Plus Mini Kit (Qiagen, Valencia, CA). Three biological replicates were prepared for each condition ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Immunology 2020Quote: ... All steps adhered to the manufacturer’s instructions and included a DNase 1 treatment (RNase-Free DNaset, Qiagen) step to remove genomic DNA contamination ...
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bioRxiv - Immunology 2020Quote: ... Isolation of RNA from THP-1 cells was carried out with a RNeasy Plus Mini kit (Qiagen). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: RNA was extracted from pollen from approximately 300 flowers of WT and dgk4-1 mutants (Qiagen, USA) and cDNA synthesized using SuperScript III reverse transcriptase according to manufacturer’s instructions (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... In brief we perform bisulfite conversion on 1 µg of genomic DNA using EpiTech kit from Qiagen according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Astrocytes were transfected with 80 nM of Rho-1 siRNA from a 20uM stock (Qiagen, Germany #SI01401743) or 80 nM AllStars Negative Control Scrambled siRNA from a 20uM stock (Qiagen #SI03650318) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting products were run on 1 % agarose gel then extracted using QIAquick Gel Extraction Kit (Qiagen). Restriction enzymes and Phusion DNA polymerase were purchased from New England BioLabs Inc and used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from 0.5-1 μg total RNA with the QuantiTect reverse transcription kit (205311; Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: The small RNAs from the yeast cells (1 x 107) were isolated with the miRNeasy kit (Qiagen) with modifications ...
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bioRxiv - Immunology 2021Quote: ... Cells were sorted in RTL Lysis Buffer plus 1% β-mercaptoethanol (74134, RNeasy Plus Mini Kit; QIAGEN), or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA from 10 Ag(PMB)1 individuals was extracted using the Blood and Tissue Kit (Qiagen). For each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of gDNA was extracted from WILD-seq transduced cells (Blood&Cell Culture DNA Kit, Qiagen) and the barcode amplified by PCR using primers containing Illumina-compatible adapters (gDNAWhitelist_Fwd/Rev) ...
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen). RT-qPCR was performed in a LightCycler480 (Roche ...
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bioRxiv - Genomics 2022Quote: ... and homogenized at 25/s for 1 min at 4°C using a TissueLyser II (Qiagen, 85300). Samples were incubated at room temperature for 5 min followed by addition of 180 μl chloroform ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with 500ng plasmid DNA and a 1:50 Effectene transfection reagent ratio (Qiagen #301425).
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared from 1 μg total RNA using QIAseq FastSelect -rRNA HMR (Qiagen 334386) and QIAseq Stranded RNA kits (Qiag ...
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bioRxiv - Microbiology 2022Quote: ... Ligation of 1 µl 5′RACE PCR products were performed with the Qiagen PCR cloning kit (Qiagen) according to the manufacters protocol ...
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bioRxiv - Immunology 2022Quote: cDNA was generated from 1 µg RNA with the Omniscript® Reverse Transcription Kit (Qiagen, Hilde, Germany) using oligoDT12-18 primer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2024Quote: ... total DNA from 1×106 cells was extracted using the Blood & Cell Culture DNA Mini Kit (Qiagen). Extracted DNA was diluted to 10 ng/μl and subjected to qPCR analysis ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen, Germany)) and qPCR was carried using the StepOnePlus machine (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen). RNA was sequenced using the DNBseq platform at the Beijing Genomics Institute (BGI Group ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was performed from 1 µg RNA using the Quantitect Reverse Transcription Kit (QIAGEN, Manchester, UK) and cDNA was diluted to 10 ng/µl equivalent before use in qPCR ...
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bioRxiv - Microbiology 2023Quote: ... RT buffer 5x (4 μL) RT primer mix and Reverse transcriptase (1 μL) (Qiagen, Cat. No. 205311) added in a final volume of 20 μL ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each PCR reaction was extracted from a 1% agarose gel and column purified using QG buffer (Qiagen), following manufacture instructions ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from cell pellets (1 million cells) using Allprep DNA/RNA mini kit (Qiagen). Whole genome libraries were sequenced 150x2 bp on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Immunology 2023Quote: ... 1×105 cells were removed four days after electroporation and DNA isolated using the DNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was carried out with 1 µg of RNA using the QuantiTect Reverse Transcription Kit (Qiagen) and subsequently diluted 1:25 in dH2O ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from 1-5E5 sorted cells using the DNeasy blood and tissue kit (Qiagen, 69504) and used for barcode amplification and NGS analysis.
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA using a QuantiTect™ Reverse Transcription kit (Qiagen) from random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of RNA was used per sample using the QuantiTect Reverse Transcription Kit (Cat# 205311, Qiagen). The quantitative RT-qPCR reactions were carried out using the PowerUp™ SYBR™ Green Master Mix (Cat# A25742 ...
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bioRxiv - Microbiology 2024Quote: ... and bead-beating them three times for 1 minute at 30 Hz using a TissueLyser II (Qiagen) and the steel beads included in the DNeasy Plant Pro Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA (gDNA) was extracted from 1×106 PBMCs using the QIAamp DNA Blood Mini Kit (Qiagen) per manufacturers’ instructions ...
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bioRxiv - Genetics 2024Quote: ... The entire HTT exon 1 coding sequence was amplified using UltraRun® LongRange PCR Kit (QIAGEN, 206442) with 10% DMSO using the following PCR conditions ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were confirmed with a 1% agarose gel and PCR purified (QIAquick PCR Purification kit, QIAGEN). The dsRNA was synthesized using a T7 reverse transcription reaction kit (MEGAscript ...
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bioRxiv - Biophysics 2020Quote: ... reaction mix (for PCR program details see Supplementary Table 1) and purified with QIAquick PCR Purification Kit (QIAGEN). The Fps1-mGFP strain was created by transforming the BY4741 background strain with 100 µL of the purified mGFP-HIS3 fragment with standard LiAc protocol to allow for homologous recombination 34 ...
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bioRxiv - Microbiology 2021Quote: ... This was purified by 1% agarose gel electrophoresis and gel extracted according to QIAquick gel extraction kit (QIAGEN) standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Total RNA was purified from eleven samples Table 1 using the QiaAmp viral RNA mini kit (Qiagen – 52906) following viral inactivation at the MRCG at LSHTM containment level 3 facility ...
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bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified from a 1% agarose gel using the QIAquick gel extraction kit (Qiagen, Inc.) and cloned in NdeI and XhoI sites of pET-22b (+ ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg total RNA was then used for first strand cDNA synthesis using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time PCR was performed using iQ SYBR Green Supermix (Bio-Rad) ...