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Citations for Qiagen :
2951 - 3000 of 4028 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 5 and 0.22 μm pore-size filters were processed using the DNeasy PowerWater Kit (QIAGEN, Hilden, Germany) following the manufacture instructions ...
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bioRxiv - Plant Biology 2019Quote: RNA was extracted from leaves of 5-week-old Arabidopsis plants using RNeasy Mini extraction kit (Qiagen) and treated with Turbo DNA-free kit (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... and 5 units lysostaphin and mutanolysin for 30 minutes prior to beadbeating in the TissueLyser II (QIAGEN) for 2 x 3 minutes at 30 Hz ...
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bioRxiv - Genomics 2021Quote: DNA was extracted from the donors’ peripheral blood (5 ml) using DNeasy Blood & Tissue Kit from QIAGEN according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 300mM NaCl and 5% glycerol and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 40mM imidazole and σA eluted with 150mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were collected for genomic DNA extraction using red blood cell lysis solution (Qiagen, 28606).
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bioRxiv - Plant Biology 2022Quote: All PCR reactions were performed with the 5-Prime Hot Master Mix (Quantabio, QIAGEN, Beverly, MA, U.S.A.). All PCR products and pooled library were purified with CleanNGS beads (CleanNA ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... The albumin-Cre allele was detected by PCR and HotStar Taq DNA Polymerase (5 U/μl, Qiagen) using the following primers ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neuron-astrocyte co-cultures at 5 weeks using RNeasy Mini kit (74104, Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: On day 5 of dietary exposure RNA was extracted via the Qiagen RNeasy Mini Kit (Qiagen 74104). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were then centrifuged (300xg, 5 min) before RNA isolation using the RNeasy Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen) or Maxiprep (Thermo-Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in 2 mL solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... at 300 cells per well into 96 well LoBind plates containing 5 μL of EB buffer (Qiagen). After sorting ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was passed through a 5 mL column volume (CV) of Ni-NTA agarose resin (Qiagen: 30250). The column was then washed with 10 CV of His binding buffer (50 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was resuspended in 340μl of lysis buffer containing 5 mM EDTA and proteinase K (Qiagen) and incubated at 55°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were constructed using Enzymatics 5× WGS fragmentation mix and WGS ligase reagents (Qiagen, Hilden, Germany), and then libraries were sequenced on HiSeq X Five platform in Macrogen Japan (Tokyo ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from 5 × 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from ∼100,000 MSNs from 4 independent differentiations per experimental group according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 72 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Genetics 2019Quote: ... cells (from 4 × 104 to 2.3 × 106) were pelleted at 400xg for 4 min and resuspended in 700 μL RLT buffer (Qiagen) with beta-mercaptoethanol (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
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bioRxiv - Genetics 2019Quote: Single cells meant for RNA processing were sorted into 96-well full-skirted Eppendorf plates that were pre-chilled at 4°C and were prefilled with 10μL of lysis buffer consisting of TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from P15 liver tissue (n=4 biological replicates per strain) using QIAzol Lysis Reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 19 motifs and 4 differential motifs were analyzed to predict upstream regulators using Ingenuity Pathway Analysis (IPA, QIAGEN Inc.). Gene expression signatures indicative of HMO and motif abundance were defined as genes differentially expressed with abundance in the previous limma analysis(FDR q<0.05 and |Fold Change|>1.5).
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bioRxiv - Plant Biology 2020Quote: ... 10mM imidazole was added and incubated on rotator for overnight at 4°C in the presence of 2.5ml of Ni-NTA beads (Qiagen). The beads were washed with EB containing 6M guanidine-HCl and 0.25% Triton X-100 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... Two hundred fifty microliters of each sample were transferred into 96-well PowerBead Plates (Qiagen, 27500-4-EP-BP). Seven hundred fifty microliters of RLT buffer from an AllPrep DNA/RNA 96 Kit (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 ng/μL RNA carrier as described by the supplier of the RNeasy® Micro kit (Qiagen, Hilden, Germany).
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bioRxiv - Genomics 2022Quote: ... samples were incubated an additional 30 minutes with 20 µL Proteinase K and 4 µL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit ...
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bioRxiv - Cancer Biology 2019Quote: ... and was run on miScript miRNA PCR Array Human Ovarian Cancer plates (Qiagen, MIHS-110ZE-4, 384 well plate). PCR plates were read the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from ~200 mg stool using the QIAGEN DNeasy PowerSoil HTP 96 Kit (Qiagen, Cat #12955-4) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Total RNA was harvested at 4 and 24 hours in biological triplicates using the RNeasy plant mini kit (Qiagen). Purified RNA was verified for intactness on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genomics 2019Quote: ... while the manual plate samples were processed using the Qiagen DNeasy PowerSoil HTP 96 kit (Qiagen, Cat# 12955-4).
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bioRxiv - Microbiology 2019Quote: ... 700 μL of cold methanol and 140 μL of EDTA 0.1M were added and vigorously mixed (4 x 45 s) in a mini-bead-beater-8 (Biospec Products, Qiagen). By this way ...
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bioRxiv - Molecular Biology 2021Quote: ... The solution was further cleared at 20,000 × g at 4 °C and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Frozen samples were homogenized with zirconia beads YTZ-4 (AS-ONE, Osaka, Japan) using TissueLyser II (Qiagen, Hilden, Germany), and total RNA was then extracted using the Maxwell 16 LEV Plant RNA Kit (Promega ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted using the Qiagen MagAttract Power Microbiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) and used for rRNA sequencing and Helicobacter spp ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from multiples of 4 million resting CD4+ T cells according to the manufacturer’s instructions (QIAamp DNA Mini and Blood Mini Kit, QIAGEN). Amplification of NFL genomes was performed by limiting dilution ...
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bioRxiv - Microbiology 2022Quote: ... The aqueous phase was separated by centrifugation at 13,000 g at 4°C and RNA was purified using the RNeasy Mini Kit (Qiagen) with on-column DNAse digestion ...