Labshake search
Citations for Qiagen :
2751 - 2800 of 4028 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 mM Tris pH 8.0) using 5 mM stainless steel beads in a TissueLyser II (Qiagen). In vitro TPP1 assay was performed as described ...
-
Establishment of Wolbachia strain wAlbB in Malaysian populations of Aedes aegypti for dengue controlbioRxiv - Microbiology 2019Quote: ... Mosquitoes were homogenised in 175 µL of 5% Chelex® solution using TissueLyser II machine (Qiagen) and with 5 µL of proteinase K (20 mg/mL ...
-
bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was applied to two tandemly-connected 5 ml Ni-NTA Superflow cartridges (Qiagen); the cartridges were washed with buffer containing 20 mM Tris pH 8.0 ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were incubated in 0.08 M KOH for 5 min and washed by Buffer EB (Qiagen). Then ...
-
bioRxiv - Microbiology 2020Quote: ... The proteolysis reaction products were then passed over a 5 mL Ni-NTA superflow cartridge (Qiagen) to remove TEV and uncleaved protein ...
-
bioRxiv - Genomics 2020Quote: ... before aliquoting into 5 tubes and proceeding with the Qiagen DNeasy Plant Mini Kit (Qiagen; 69104) protocol ...
-
bioRxiv - Biophysics 2022Quote: ... to dephosphorylate 5’ ends.Digested inserts were gel extracted using the QiaQuick gel extraction kit (Qiagen, Germany). Digested and 5’ dephosphorylated vector was purified using the QiaQuick PCR Cleanup kit (Qiagen ...
-
bioRxiv - Developmental Biology 2022Quote: ... total RNA from 5 embryos was extracted using the RNeasy microRNA isolation kit (Qiagen, Valencia, CA), and the RNA samples were digested on-column with RNase-free DNase I to eliminate genomic DNA ...
-
bioRxiv - Genomics 2022Quote: We extracted RNA from 5 × 105 cells using the QIAGEN RNeasy Mini kit (Qiagen, cat # 74014) with DNase I treatment (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
-
bioRxiv - Microbiology 2024Quote: ... One µg of purified RNA was treated twice with 5 µl of RNAse-free DNAse (Qiagen) and subjected to a final on column purification ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was prepared from approximately 5×106 cells using the DNeasy-96 kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was transferred to a fresh tube and 5 ml of Ni-NTA resin (Qiagen) was added.
-
bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
-
p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... The p110α deletion was confirmed by PCR and HotStar Taq DNA Polymerase (5 U/μl, Qiagen) using two primer pairs ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 μL of each reaction mixture was used for amplification with the REPLI-g kit (Qiagen) at 16 °C overnight and cleaned with the DNA Clean & Concentrator kit to yield samples for sequencing ...
-
bioRxiv - Genomics 2024Quote: ... 100 mg of frozen needles were cut into < 5 mm pieces and disrupted using TissueLyser (Qiagen) at 30 1/s for 2×120 s ...
-
bioRxiv - Microbiology 2024Quote: ... left still for 5 min and transferred to a RNeasy spin column (RNeasy Mini kit, QIAGEN). The column was washed two times with RPE buffer before elution in RNA-free water ...
-
bioRxiv - Genomics 2021Quote: ... was run in a 1% agarose gel and the desired band (1.8 kb) was purified using QIAquick gel extraction kit (Qiagen, Cat. No. 28704) and used as a template for the transcription reaction ...
-
bioRxiv - Microbiology 2021Quote: RNA was extracted from the supernatant of cells infected with Candid #1 (P0 and P11) using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
-
bioRxiv - Developmental Biology 2020Quote: ... Japan) and 1 μL of the cDNA synthesis reaction mixture was used with the Platinum SYBR Green kit from Qiagen (Hilden, Germany). q-PCR was performed using a Rotor-Gene Q Real-time PCR machine (Qiagen ...
-
bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were resolved on a 1% w/v agarose gel and purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Zoology 2020Quote: Adult Spadella cephaloptera were starved for three days and sacrificed together with developmental stages covering early zygotes to hatched 1 months old juveniles for RNA extraction by using a RNA extraction kit (Qiagen, Roermond, Netherlands). Additional adults and developmental stages were carefully anesthetized in 7.14% MgCl2 before fixation and fixed for 1 hour at room temperature for in situ hybridization experiments and treated as previously described (Wollesen et al ...
-
bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
-
bioRxiv - Bioengineering 2021Quote: ... Membranes were blocked with 5% milk in PBST (Phosphate buffer saline, 0.05% Tween 20) and incubated with monoclonal mouse anti-His antibody (Penta His, Qiagen, dilution 1:1000) according to the manufacturers’ instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
-
bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... Compost was crushed using mortar and pestle under liquid nitrogen and ground to a fine powder for 1 min at 30 Hz using a tissuelyser II with a steel grinding jar pre-cooled with liquid nitrogen (Qiagen, Venlo, Netherlands). RNA was extracted using a modified method described by Patyshakuliyeva et al ...
-
bioRxiv - Microbiology 2020Quote: Genomic DNA of strains IMX2300 and IMX2300-1 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
-
bioRxiv - Microbiology 2022Quote: ... Samples and the DNA extraction negative control (1 ml PBS) were processed for DNA extraction using the QIAamp DNA Microbiome kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Depletion of Host DNA protocol).
-
bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour at room temperature either with a commercial blocking buffer (QIAGEN, anti RGS HIS6 HRP conjugate kit) or with 5% milk in 0.05% tween TBS buffer ...
-
Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Each primer (3.5 μl) was combined with reverse-transcriptase reagent (1 μl) and SYBR® Green master mix (25 μl) (Qiagen, Valencia, CA). The thermal profile was as follows ...
-
bioRxiv - Microbiology 2020Quote: ... 120 mg l-1 sodium pyruvate and 10% FCS (supplemented with 10% Fetal Calf Serum and 1% penicillin–streptomycin) at 300 Hz for two minutes using the Tissuelyser II (Qiagen, Hilden, Germany) and centrifuged to clarify the supernatant ...
-
bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
-
bioRxiv - Molecular Biology 2019Quote: DNA was purified from the eluate and from 1% and 10% input chromatin using QIA-Quick DNA purification kit (Qiagen, Hilden, Germany) and eluted within 50 μl of ddH2O ...
-
bioRxiv - Molecular Biology 2019Quote: ... a sample from each tested swatch was collected using a 4N6 FLOQSwab® (Copan Diagnostics) and DNA extracted using a Qiagen EZ-1 instrument (Qiagen, Netherlands). One side of a 4N6 FLOQSwab was wetted with 2 μl of molecular biology grade water and rubbed over the surface of the swatch ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... All samples were diluted to 1 µg of total RNA and were used to synthesize cDNA using a QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) in accordance with manufacturer protocols ...
-
bioRxiv - Bioengineering 2020Quote: ... pelleted and lysed (Qiagen RLT lysis buffer supplemented with 1% β-Mercaptoethanol. RNA was recovered using spin columns (Qiagen MicroRNAeasy® kit), with on-column DNAse I digest performed according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... For the detection of phosphorylated Serine residues a TBS solution with a 1:100 dilution of the Anti-Phospho-Serin Antibody (Qiagen, Hilden, Germany) was used ...
-
bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
-
bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
-
bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from the liver of an adult female Limnodynastes dumerilii dumerilii (Fig. 1) using the Gentra Puregene Tissue Kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions with the following exceptions ...
-
bioRxiv - Genetics 2020Quote: ... at least ∼1 × 107 cells were collected by centrifugation and RNA was purified using an RNeasy Mini Kit (Qiagen 74104; Hilden, Germany), including on-column DNase digestion (Cat No ...
-
bioRxiv - Microbiology 2020Quote: High-quality genomic DNA was obtained starting from 250 mg of each sample of table 1 using DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany), according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... DNA was extracted from up to 1 gram of sediment using a modified version of the Qiagen DNeasy PowerSoil kit (Qiagen, Venlo, Netherlands), as described previously [41 ...