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Citations for Qiagen :
3201 - 3250 of 4028 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... A 5 ml aliquot of culture was removed and added to 10 ml of RNAprotect Bacteria Reagent (Qiagen) and mixed by vortexing ...
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bioRxiv - Microbiology 2023Quote: ... Edmund Bühler) by bead beating twice for 5 min at 30 Hz in a Tissue Lyzer II (QIAGEN). Lysates were then incubated for 30 min at 37 °C with shaking ...
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bioRxiv - Plant Biology 2023Quote: Total RNA from 5-day-old whole plants was extracted using the RNeasy Plant Mini Kit (Qiagen, 74904). For RNA-seq ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 5-10 g of soil per sample with the DNeasy PowerMax Soil Kit (Qiagen) and used for short read and long read sequencing ...
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bioRxiv - Physiology 2023Quote: ... a 5 mg piece of kidney tissue was homogenized in 350 μL of RNeasy RLT Lysis buffer (Qiagen) containing 2-mercaptoethanol and centrifuged at maximum speed for 3 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Sequences with an E-value lower than 1e-5 were used for multiple sequence alignment using CLC v 21.0.5 sequence manager (Qiagen). After multiple rounds of alignments and manual removing non-nAChR sequences a set of 2047 proteins were obtained ...
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bioRxiv - Biochemistry 2023Quote: ... High speed supernatant (HSS) was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4ºC for 2 hours stirring in a beaker ...
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bioRxiv - Immunology 2023Quote: RNA was purified from at least 5 x 104 CD4+ T cells using the RNeasy Micro kit (Qiagen). cDNA was synthesized using the SuperScriptTMVILOTMcDNA synthesis kit (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Each of the wells that the nuclei were sorted into contained 5 uL of EB buffer (Qiagen, 19086), 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... Single blastocysts were collected from the M2 drop in 5 µL of 1x TCL lysis buffer (Qiagen #1031586) containing 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: All muscles were homogenized 2 × 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg total RNA was used for cDNA synthesis using an Omniscript Reverse Transcription Kit (Qiagen). For the quantitative real-time PCR ...
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bioRxiv - Microbiology 2021Quote: ... except that 2 mL prefilled PowerBead tubes (glass beads, 0.1 mm; Cat no. 13118-50, Qiagen) were used for the bead beating ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Ni+2-NTA agarose affinity chromatography according to standard protocol (Qiagen).
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples (15-30 mg) were transferred to 2 ml round-bottom tubes (Qiagen, Hilden, Germany) with the addition of a 5 mm stainless steel bead (Qiagen ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... as per the manufacturer and a final elution step of 2×10 μL EB buffer (Qiagen).
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bioRxiv - Genomics 2021Quote: ... counted and 2×104 were lysed in 100 µL of lysis buffer (RLT Plus (Qiagen, 1053393) and 1% ß-mercaptoethanol (Sigma-Aldrich,M3148)
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bioRxiv - Microbiology 2021Quote: ... Tissues were homogenized at 30 Hz for 2 min using TissueLyser II (Qiagen GmbH, Hilden, Germany) and centrifuged for 30 sec at 11000 rpm ...
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bioRxiv - Bioengineering 2020Quote: ... tissue clay and control hydrogels were homogenized for 2 minutes (TissueRuptor) in QIAzol lysis Reagant (Qiagen) and chloroform was added to precipitate and remove proteins ...
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bioRxiv - Plant Biology 2021Quote: ... and the resultant supernatant was mixed with 2 ml of Ni-NTA agarose resin suspension (Qiagen) and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen tissue was thawed and immediately homogenized in 2 ml of RTL buffer + 2mM DTT (Qiagen). mRNA was extracted from 300 ul of homogenate using the RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was bound to a 2 mL slurry of Strep-Tactin Superflow plus (Qiagen) overnight at 4°C using the batch method ...
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bioRxiv - Physiology 2021Quote: ... Pooled libraries were diluted to 2 nM in EB buffer (Qiagen, Hilden, Germany, Cat. No. 19086) and then denatured using the Illumina protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extract was subjected to IMAC by incubation with 2 ml Ni-NTA resin (Qiagen, Germany) per 50 ml of extract ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Each 25 µL qPCR reaction contained 12.5 µL of 2× QuantiTect Probe PCR Master Mix (QIAGEN), 600nM of each primer ...
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bioRxiv - Neuroscience 2022Quote: ... CD11b+ CD45+ cells from one animal (2 retinas) were sorted directly into RLT buffer (QIAGEN 79216) and stored at -80°C ...
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bioRxiv - Genomics 2022Quote: ... Purified PCR products (400 ng) were mixed with 2 μL 10X Taq polymerase PCR buffer (QIAGEN) and nuclease free water to a final volume of 20 μL ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... 106 cells were then transfected with 2 μg of DNA using Effecten® transfection reagent (Qiagen) according to the manufacturer’s instructions at a 1:10 DNA/Effecten® ratio ...
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bioRxiv - Microbiology 2021Quote: ... and gel purified on a 2% agarose gel using Qiagen Gel Purification Kit (Qiagen, Frederick, MD). After quality check using 2100 Bioanalyzer and DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from the 2 µm filters using the DNeasy PowerWater kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR of cDNA samples was performed using 2× Rotor-Gene SYBR Green PCR Mastermix (Qiagen) and Rotor-Gene Q real-time PCR cycler (Qiagen).
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bioRxiv - Molecular Biology 2020Quote: Cells from 1.0 ml of culture were mixed with 2 ml of RNA protect reagent (Qiagen). The mixture was pelleted at 4000 rpm for 10 minutes and then discard the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was then incubated with 2 ml Ni-NTA Superflow resins (Cat No.30430, QIAGEN) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Primers sequences are shown in Extended data table 2 and predesigned quantitech primer assays (Qiagen, 249900) were used for other genes including Gal (QT00109970) ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was extracted from 2 × 106 cells per duplicate using DNeasy Blood & Tissue Kits (QIAGEN) and sheared to 100-300 bp with a sonicator ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenate was then centrifuged at full speed for 2 min in QIAshredder column (Qiagen, 79656). RNA was extracted from the flow-through using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Genetics 2019Quote: ... DNA was separated by 2% agarose gel electrophoresis and mononucleosome bands were gel-extracted (Qiagen MinElute) and converted to genomic libraries using the Ovation Ultralow Library System V2 (NuGEN ...
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bioRxiv - Genomics 2021Quote: ... PCR products were run on a 2 % gel and purified using QIAquick Gel extraction kit (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... size selected on a 2% agarose gel and purified with a QIAquick Gel Extraction Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... for 1h at 37C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). In parallel ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS- CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA from 2×107 cells was extracted by DNeasy blood & tissue kit (Qiagen, Cat.69504) and sgRNA cassettes were PCR amplified for illumina NGS 53.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 2-ml column of Ni-NTA-agarose (Qiagen, Hilden, DE) at a flow rate of 1 ml min−1 ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the Caco-2 cell lines using a RNeasy Mini Kit (QIAGEN SA) with a DNase I step to eliminate DNA contaminants ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from iPSC-derived microglia (Supplementary Table 2) was extracted using RNeasy Plus kit (Qiagen). Library construction and sequencing were performed by Novogene ...