Labshake search
Citations for Qiagen :
201 - 250 of 998 citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2022Quote: ... The N gene-specific primers were used to amplify 97 bp of SARS-CoV-2 N gene by conventional PCR and purified by Qiagen gel extraction kit (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: 3-5 × 105 cells with or without treatment with IFNα and SARS-COV-2 infection were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
-
bioRxiv - Immunology 2022Quote: Sorted CD14+ monocytes from total PBMC either ex vivo or after a 20 hour stimulation with 106 UV-inactivated SARS-CoV-2 viral particles per 106 cells were lysed with RLT Plus buffer (QIAGEN). RNA was isolated using the RNeasy Micro Plus Kit (QIAGEN ...
-
bioRxiv - Microbiology 2021Quote: 3-5 x 105 cells infected or not with SARS-CoV-2 or HCoV-NL63 were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
-
bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
-
bioRxiv - Genomics 2020Quote: ... and Consensus Sequence generation RNA was extracted from eight respiratory samples positive for SARS-CoV-2 using the QIAmp Viral RNA Mini kit (Qiagen). Sequencing was performed as described previously (11) ...
-
bioRxiv - Microbiology 2022Quote: Fecal samples positive for the partial E gene of sarbecovirus were homogenized in TissueLyser II (Qiagen) using 0.1 mm glass beads (Tomy Seiko ...
-
bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 viruses were purified from the clinical samples by using QIAamp Viral RNA Mini Kit (Qiagen, Cat. No. 52906). The preparations were analyzed by real-time RT–PCR testing for the determination of viral titers of SARS-CoV-2 by standard curve analysis ...
-
bioRxiv - Genomics 2020Quote: The SARS-Co-V-2 viral target genome amplicon libraries were constructed using the QIAseq SARS-CoV-2 Primer Panel V2 (Qiagen, Germany) coupled with QIAseq FX DNA library kit (Qiagen ...
-
bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
-
bioRxiv - Microbiology 2022Quote: ... hACE2 expression or SARS-CoV-2 nucleocapsid (N) gene in individual mouse organs was determined using QuantiNova SYBR Green PCR kit (Qiagen #208052) in combination of 500 nM of hACE2 gene specific primer set (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 viriants S and point-mutated pseudovirus were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Cat#52906), and served as template for reverse transcription using the TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR reagent (TransGen Biotech ...
-
bioRxiv - Microbiology 2023Quote: RNA was extracted from a SARS-CoV-2 Omicron BA.1 clinical isolate (SARS-CoV-2/human/USA/CA-CDC-4358237-001/2021) (GenBank: OM264909.1) using the QIAamp Viral RNA kit (Qiagen, Hilden, Germany). The complete wild-type Omicron genome was then reverse transcribed via RT-PCR with SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: The total RNA was extracted from uninfected and SARS-CoV-2 infected mice lung tissue using RNeasy mini kit (QIAGEN #74104). The quantity of RNA was determined using Qubit RNA assay kit with Qubit 4.0 and the quality of RNA was tested using agarose gel electrophoresis and High Sensitivity Tape station Kit (Agilent 2200 ...
-
bioRxiv - Neuroscience 2021Quote: ... Plasmids were amplified in Escherichia coli DH5a and purified using HiSpeed Plasmid Maxi Kit (Qiagen).
-
bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
-
bioRxiv - Microbiology 2020Quote: ... coli C600 strain using an Endofree Maxi-Prep kit from Qiagen (QIAGEN, catalog number 12362) following the manufacturer’s recommendations.
-
bioRxiv - Cell Biology 2020Quote: ... induced in BL21-CodonPlus(DE3)-RIL Escherichia coli and affinity purified with Ni2+ agarose (Qiagen). The purified His6Tam41 protein was used as an antigen in rabbits (Pacific Immunology) ...
-
bioRxiv - Microbiology 2021Quote: ... coli using a QIAprep Spin Miniprep Kit according to manufacturer’s instructions (Qiagen, Germany, Hilden, Germany). The phage promoter eGFP expression cassette (pCMP1::eGFP ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli following standard procedures and purified by using the Qiagen Miniprep Kit (Qiagen, Maryland, USA). All sequences and constructs were checked by sequencing before further use ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli and then extracted and purified using a plasmid purification maxi kit (Qiagen, Valencia, CA). Purity was assessed by the ratio of absorbance at 260 and 280 nm ...
-
bioRxiv - Cell Biology 2019Quote: ... coli BL21(DE3) and purified on Ni-NTA-agarose as described by the manufacturer (Qiagen). Before elution an additional washing step with ATP was performed.
-
bioRxiv - Plant Biology 2020Quote: ... coli was used for cDNA synthesis employing QuantiTect® Reverse Transcriptase Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... coli lysates under native conditions by immobilised metal affinity chromatography using Ni-NTA agarose (Qiagen) essentially as described 12 using 50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... coli DH5α cells and isolated through the QIAprep Spin Miniprep Kit (Qiagen; Cat. No. 27106). All the cloned plasmids (Supplementary Table S3 ...
-
bioRxiv - Biochemistry 2022Quote: ... coli lysates under native conditions as described in the fifth edition of the QIAexpressionist (Qiagen). The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare ...
-
bioRxiv - Microbiology 2023Quote: ... coli-specific adaptations of the RNA extraction protocols in the RNAProtect Bacteria Reagent Handbook (QIAGEN). Following RNA extraction ...
-
bioRxiv - Immunology 2023Quote: ... The PCR fragments were ligated into an Escherichia coli expression vector (pQE30; Qiagen, Hilden, Germany) to create a protein construct with a six-tandem histidine residue (His-tag ...
-
bioRxiv - Cancer Biology 2022Quote: ... coli following ampicillin selection using the QIAGEN Plasmid Purification Midi-Prep Kit (QIAGEN, Hilden, Germany) and reconstituted in sterile Tris-EDTA (TE ...
-
bioRxiv - Molecular Biology 2020Quote: ... Brains were extracted and sliced on a matrix and the middle third of the left cerebral hemisphere was lysed in Qiazol (Qiagen, Hilden, Germany, Catalog# 79306). RNA was extracted using the miRNeasy micro kit (Qiagen ...
-
bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...
-
bioRxiv - Pathology 2022Quote: Total RNA was extracted from the lungs of uninfected and SARS-CoV-2 infected hamsters with or without immune suppression using Trizol reagent and purified by RNeasy mini columns (Qiagen, CA, USA) as described previously 61 ...
-
bioRxiv - Microbiology 2020Quote: We evaluated the impact of using Nanotrap particles to capture and concentrate SARS-CoV-2 on several nucleic acid extraction methods: the QIAamp® Viral RNA Mini Kit from QIAGEN (52906); the RNeasy® Mini Kit from QIAGEN (74106) ...
-
bioRxiv - Immunology 2022Quote: ... of soluble SARS-CoV-2 WA1 and SARS-CoV-2 Omicron BA.1 spike trimers were isolated from cell supernatants using a Ni-NTA column (Qiagen, Hilden, Germany). Eluents from Ni-NTA purifications were subjected to SEC using a HiLoad Superdex 200 16/600 column followed by a Superose 6 10/300 (Cytiva ...
-
bioRxiv - Biochemistry 2022Quote: ... The peptide spot membrane was blotted onto a nitrocellulose membrane and UNC-45 binding was probed with an anti-Strep antibody (Qiagen) using an ECL-kit for detection (GE Healthcare).
-
bioRxiv - Bioengineering 2020Quote: ... coli bacteria (as described in section 2.1) and extracted using a Plasmid Maxi kit (Qiagen, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... coli isolate using a modified version of the Qiagen DNeasy PowerLyzer microbial kit (Qiagen, Hilden, Germany). Using a 10μl loop ...
-
bioRxiv - Plant Biology 2024Quote: ... coli under a T7 promoter and purified using Ni-NTA nickel affinity beads (Qiagen, Hilden Germany). Purified protein was dissolved in PBS solution and lyophilized ...
-
bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from serially diluted and the 4 different media SARS-CoV-2 samples using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Briefly ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli cultures at early growth states (30 min post inoculation) using the RNeasy Mini kit (Qiagen: #74106) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... coli BL21 Star (DE3) (Invi-trogen) using genomic the Blood and Cell Culture DNA mini kit (Qiagen). The amplified PCR product was digested in one step using NdeI and BamHI HF (New England Biolabs ...
-
bioRxiv - Biophysics 2022Quote: ... coli colonies were collected with scrapers and the library plasmids were purified using Plasmid Maxi Kit (Qiagen). Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences) ...
-
bioRxiv - Bioengineering 2023Quote: ... coli and purified for cell culture application through commercially available kits (Qiagen, Maxi Plasmid Kit, Cat# 12162).
-
bioRxiv - Cell Biology 2020Quote: ... Approximately 60 homozygous males from each E-box deleted fly line were disrupted in lysis-buffer (Buffer AL, Qiagen) using a motorized microtube homogenizer ...
-
bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
-
bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
-
bioRxiv - Cancer Biology 2023Quote: ... Protein-protein interaction networks were built using Ingenuity Pathway Analysis (IPA) (Qiagen).
-
bioRxiv - Neuroscience 2021Quote: ... Solubilised membranes were incubated with Ni-NTA resin (Qiagen) (1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... on-membrane DNase I (RNase-Free DNase Set, Qiagen) digestion was performed according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2023Quote: ... Then the membranes were DNase treated (DNase I, Qiagen) for 15 min at room temperature ...