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Citations for Qiagen :
1 - 50 of 998 citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... coli and purified with NiNTA matrix (Qiagen). Immunization of the rabbits was performed by Absea (Beijing ...
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bioRxiv - Biochemistry 2019Quote: Mutant Chinese hamster BiP proteins with an N-terminal His6-tag were purified as described before with modifications (Preissler et al, 2017b). Proteins were expressed in M15 Escherichia coli (E. coli) cells (Qiagen). The bacterial cultures were grown in LB medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin at 37 ° C to an OD600nm of 0.8 and expression was induced with 1 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 ml of Matrigel basement membrane matrix (Qiagen #354234) prepared in serum free culture medium (1:8 ratio to reach final concentration of 1.2 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... or pQE30 vectors (expressed in M15 E. coli cells (Qiagen)) ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg C19orf43 (purified from E. coli BL21 through Qiagen Ni-TA agarose using standard procedures ...
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bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Verified plasmids were extracted from Escherichia coli (E. coli) using the QIAprep Spin Miniprep Kit or HiSpeed Plasmid Midi Kit (Qiagen) and dialyzed (0.025µM MCE Membrane ...
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bioRxiv - Microbiology 2020Quote: Coronavirus nsp12 sequence alignment was generated using CLC Workbench (Qiagen) from sequences downloaded from the NCBI website (Accession numbers MN985325.1 and MT123290.1 ...
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bioRxiv - Microbiology 2022Quote: ... 1mg of bacterial genomic DNA (isolated from E. coli cells using genomic DNA kit, Qiagen), or tRNA (from E ...
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bioRxiv - Microbiology 2021Quote: The cells were lysed by high speed vortexing for 2 minutes using lysing matrix E tubes (MP Biomedical) in buffer RLT (Qiagen) amended with 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... Samples initially underwent a mechanical lysis step by adding samples to a Lysing Matrix E tube and placed within a Tissuelyzer II (QIAGEN), which were lysed for 1 minute at 30 Hz ...
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bioRxiv - Microbiology 2023Quote: ... preceded by a 10-minute bead-beating step at 30 Hz in 2 ml e-matrix tubes (MP Biomedical, USA) using a Tissuelyser II (Qiagen). Molarity and fragment-length distribution of the extracts were measured using a Tapestation ...
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bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing hexahistidine-tagged proteins was passed over a column of Ni2+-NTA matrix (Qiagen) at 0.5 mL matrix / 1 L of culture ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose, Qiagen) and used to raise polyclonal antibody in rabbits (Eurogentec) ...
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bioRxiv - Developmental Biology 2023Quote: ... coli was modified from the protein purification protocol of The QIAexpressionist (QIAGEN) as follows ...
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bioRxiv - Synthetic Biology 2023Quote: Bacterial clones on selection plates were scraped and pooled to extract recombinant plasmids containing the recipient barcodes and DNA blocks (donor barcodes, oligonucleotides, and E. coli ORFs) using Plasmid Plus Mini Kit (QIAGEN). The pooling capacity is determined by the number of unique positioning barcodes ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Microbiology 2019Quote: ... purified using Ni-NTA agarose matrix (Qiagen) in the presence of 6M urea ...
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bioRxiv - Biochemistry 2024Quote: ... coli (QIAGEN) or phage T4 SSB (gp32 ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 specific T cell immune responses were evaluated by QuantiFERON SARS-CoV-2 (Qiagen) [36] ...
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bioRxiv - Molecular Biology 2021Quote: Beta-coronavirus RNA was extracted from the viral samples on each petri dish using the QiAmp Viral RNA mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 whole-genome sequencing was performed by using QIAseq DIRECT SARS-CoV-2 Kit (QIAGEN). The quality of paired-end reads obtained from MiSeq sequencing was analyzed by using Qiagen CLC Genomics Workbench 22.0.1 and the Identify ARTIC V3 SARS-CoV-2 Low Frequency and Shared Variants (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (Qiagen) to generate the protein expression strains ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Qiagen). The bacterial cultures were grown in LB medium with 100 μg/ml ampicillin and 50 μg/ml kanamycin at 37°C to an OD600 nm of 0.8 and expression was induced with 1 mM isopropylthio β-D-1-galactopyranoside (IPTG) ...
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bioRxiv - Biophysics 2021Quote: ... coli M15 (Qiagen) was transformed with ApsAC (1-577):pQE70-2Z.4 and grown in LB media to OD600 ≈0.7 ...
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bioRxiv - Microbiology 2023Quote: The amino acid sequences of coronavirus spike orthologues were subjected to multiple alignment using CLC Workbench 7 (CLC Bio, Qiagen, Aarhus, Denmark). Protein sequence NCBI reference sequences for the indicated viruses are as follows ...
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bioRxiv - Systems Biology 2021Quote: RNA isolation from SARS-CoV and SARS-CoV-2 infected A549-ACE2 cells was performed as described above (Qiagen). 500 ng total RNA was used for reverse transcription with PrimeScript RT with gDNA eraser (Takara) ...
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bioRxiv - Molecular Biology 2024Quote: ... soluble fraction and Ni-NTA matrix agarose (Qiagen, Germany) were kept for binding for two hours at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated from cultures infected with rVSV/SARS-CoV-2/GFP1D7 and rVSV/SARS-CoV-2/GFP2E1 using the QIAamp Viral RNA mini kit (Qiagen) and cDNA synthesis was performed using SuperScript III using hexamers (ThermoFisher) ...
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bioRxiv - Biophysics 2020Quote: ... proteins were expressed in Escherichia coli strain BL21 (DE3) and purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) followed by ion exchange chromatography on an Äkta system (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... coli M15 cells (Qiagen) with pRAREII (Novagen).
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bioRxiv - Biochemistry 2020Quote: ... coli M15 (pREP4) (Qiagen), fresh transformants were inoculated into LB liquid culture (1000 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... coli M15 cells (Qiagen) using H2O-based M9 medium supplemented with 15NH4Cl and D-glucose-13C6-d7 ...
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bioRxiv - Microbiology 2022Quote: ... coli by miniprep (Qiagen), and the internal 504 bp of pilT was removed by digestion with AccI (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... coli strain M15 (Qiagen), and recombinant proteins were produced from the E ...
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bioRxiv - Genomics 2020Quote: The SARS-Co-V-2 viral target genome amplicon libraries were constructed using QIAseq SARS-CoV-2 Primer Panel V1 (Qiagen, Germany) coupled with QIAseq FX DNA library kit (Qiagen ...
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bioRxiv - Bioengineering 2019Quote: ... Immobilized metal affinity chromatography (IMAC) using Ni-NTA matrix (Qiagen) was employed for purification purposes ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were blotted onto PVDF membrane and examined with an RGS-His4-antibody (Qiagen) and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3 ...
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bioRxiv - Biophysics 2020Quote: ... coli expression vector pQE80 (Qiagen). Expression of TEV protease and I86-HaloTag-TEV-I87 was induced in BLR (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain M15 [pREP4] (Qiagen) containing a pQE-30 derivative encoding His6-PA1048 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BLR(pREP4) cells (Qiagen) cultured in DYT medium (1% tryptone ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain M15 [pREP4] (Qiagen) containing pAJD2948 was grown in 500 mL of LB broth at 37°C to an OD600 of 0.6-1.0 ...
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bioRxiv - Microbiology 2023Quote: ... coli strain M15 [pREP4] (Qiagen) containing plasmid pAJD3179 encoding His6-PA3978 was grown in LB broth to mid-log phase at 37°C with aeration ...
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bioRxiv - Microbiology 2023Quote: ... coli strain M15 [pREP4] (Qiagen) containing a plasmid pQE-30 protein-encoding derivative was grown in LB broth to mid-log phase at 37°C with aeration ...
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bioRxiv - Biochemistry 2023Quote: ... coli from pQE80L-Navi (Qiagen) as N-terminally His-tagged and biotinylated proteins for SPR ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Biochemistry 2020Quote: Crystallization conditions were screened using commercially available sparse-matrix screens (Qiagen) and a protein concentration of 4.7 mg mL−1 ...
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bioRxiv - Systems Biology 2020Quote: ... RNA and protein fractions using silica-membrane spin columns from the AllPrep DNA/RNA/Protein kit (cat# 80004, Qiagen, Chatsworth, CA, USA). PBMCs were processed in a randomized order ...