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Citations for Qiagen :
301 - 350 of 998 citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... All the plasmids were transformed in to Escherichia coli DH10B electro-competent cells selected with appropriate antibiotics and purified using a Qiaprep Spin Miniprep Kit (Qiagen). Positive clones were transformed in Agrobacterium tumefaciens strain GV3101 and used in infiltrations for transient expression experiments.
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bioRxiv - Microbiology 2021Quote: ... The new plasmid construct (pSLP15) was transformed into Escherichia coli DH5α chemically competent cells and prepped using the QIAprep spin miniprep kit (Qiagen). Plasmid concentrations were determined using the Qubit dsDNA broad range assay kit with a Qubit 3 fluorometer (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... coli HST04 dam-/dcm- strains in which target MTases were transformed were extracted using the DNeasy UltraClean Microbial Kit (QIAGEN) according to the supplier’s protocol after induction of gene expression ...
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bioRxiv - Microbiology 2022Quote: ... coli cells were then grown to isolate the pooled plasmid library by using a Plasmid Midi kit (Qiagen, Germantown, MD) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: During this time a stock of genomic DNA (gDNA) from Escherichia coli (REL606) from ∼72 mL of overnight culture was made using the “DNeasy Ultraclean Microbial kit” (Qiagen), resulting in a stock of ∼30 mL of gDNA at 20 μg/mL (stored at -20 °C) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids pGS001 and pOXC101(33) were extracted from Escherichia coli DH5α and β3914 respectively using the QIAprep Spin Miniprep Kit (Qiagen). A tac promoter ...
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Molecular Biology 2023Quote: Total proteins from P5 hippocampi were purified with AllPrep DNA/RNA/Protein Mini Kit (Qiagen, 80004). The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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bioRxiv - Physiology 2020Quote: ... protein was extracted (Qiagen Tissue Lyser) from ∼50 mg frozen liver in tissue lysis buffer 80 ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL Protein Precipitation Solution (QIAGEN) was added ...
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bioRxiv - Cancer Biology 2023Quote: Advanced Protein Purification buffer (APP; Qiagen): the APP buffer contains zinc chloride to precipitate protein at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and The Protein Complex Suite (Qiagen). One microliter of 4.6 mg/ml protein sample suspended in crystallization buffer (25 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Crtc2 and GFP expression plasmids were purified from DH5a Escherichia coli cultures using an EndoFree plasmid kit (Qiagen, Valencia, CA, USA), and resuspended in 71 mM sterile PBS ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21(DE3)(Novogen Inc.) essentially as described [34] except that after elution from Ni-NTA agarose resins (Qiagen Inc.) by incubating with 400 mM imidazole for 1 hr at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... we cultured 250 ml Escherichia coli bacteria bearing transformed plasmids and isolated the plasmids using Qiagen Maxi-Prep Endotoxin-free kit (Qiagen; 12362) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 128° 11’ 17.2” E) and the genomic DNA was extracted from the whole bodies using DNeasy® Blood & Tissue kit (Qiagen, GmbH, Hilden, Germany). Illumina libraries for whole bodies were constructed using the TruSeq Nano Sample Prep kit (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... Electroblotted membranes were incubated with penta-His antibody (Qiagen, Valencia, CA, USA). The antigen–antibody complex was visualized with alkaline phosphatase-linked to anti-rabbit IgG followed by staining with 5-bromo-4-chloroindol-2-yl phosphate and nitro blue tetrazolium [36].
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bioRxiv - Physiology 2021Quote: ... and purified by silica-membrane columns using the RNeasy Micro Kit (Qiagen). For heart ...
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bioRxiv - Systems Biology 2020Quote: ... The membranes were scraped and samples were homogenized using a QIAshredder (Qiagen) and the proteins were precipitated with acetone ...
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bioRxiv - Systems Biology 2020Quote: ... The membranes were scraped and samples were homogenized using a QIAshredder (Qiagen). Protein concentrations were measured using the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were prepared using silica-membrane based kit (Plasmid Miniprep Kit, Qiagen) following the manufacturer’s instructions and quantified using Nanodrop Spectrophotometer ...
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bioRxiv - Immunology 2022Quote: ... and residual genomic DNA was removed by on-membrane DNase digestion (Qiagen). The total RNA samples were sent to GENEWIZ for PolyA selection ...
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bioRxiv - Neuroscience 2023Quote: ... DNase digestion was performed on the column membrane using DNase I (Qiagen) for 15 min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Peritoneal membrane sections (80mg tissue) were dissociated in 1ml buffer RLT (QIAGEN) supplemented with β-mercaptoethanol (1:100 v:v ...
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bioRxiv - Genomics 2019Quote: ... genomic DNA and protein using AllPrep DNA/RNA/Protein Mini Kit according to the manufacturer’s instructions (Qiagen). Stable and Dox-inducible expression cell line was generated by transfecting the indicated PiggyBac vectors in the presence of hyperactive transposase plasmid (hyPBase ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA and proteins were recovered by using the Allprep DNA/RNA/Protein Mini Kit (Qiagen 80004). The efficiency of Cre-induced recombination in Appflox/flox mice was verified by PCR with primers F ...
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bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extraction was done using Qiagen AllPrep DNA/RNA/Protein isolation kit (Qiagen Inc, USA). RNA purity was confirmed with 260/280 OD ratio using Nanodrop reader (Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... some samples were lysed for total protein extraction using Qproteome Mammalian Protein Prep Kit (Qiagen, Hilden, Germany). Western blot was done using capillary electrophoresis based system Wes™ (ProteinSimple ...
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bioRxiv - Biochemistry 2020Quote: ... All UNG constructs were expressed in the Escherichia coli BL21(DE3) ung-151 strain and purified using Ni-NTA affinity resin (Qiagen, Hilden Germany) as described previously (Róna et al. ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from Escherichia coli MG1655 was isolated using the PowerLyzer® UltraClean® Microbial DNA Isolation Kit from Qiagen (Hilden, Germany). Bacterial cells were harvested and lysed using glass microbeads ...
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bioRxiv - Pathology 2021Quote: ... coli 62-57nal using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer’s specifications for bacterial cells (Qiagen, Cat No. 69504). Isolated genomic DNA was sequenced on the Illumina MiSeq platform using Illumina V2 500 cycle reagent chemistry generating paired-end 250 bp reads ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... hirae V-ATPase was expressed recombinantly in Escherichia coli and purified by affinity purification with a Ni+-NTA (nitrilotriacetic acid) column (Ni+-NTA Superflow; Qiagen, Hilden, Germany). The column was preconditioned with buffer consisting of 50 mM potassium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... coli following a standard heat shock protocol then isolated by miniprep (Cat. No. 27104, QIAprep Spin Miniprep Kit, Qiagen, Germantown, MD, USA) following the manufacturer’s instructions and eluted in molecular grade water to minimize the salt concentration for electroporation into N ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins from HGCC cells and GBM tissue were extracted with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Neuroscience 2019Quote: ... RNA and protein were extracted for ECM composition analysis using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was processed with the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Systems Biology 2019Quote: ... To summarize the cellular locations and protein classes the protein list was annotated using Ingenuity Pathway Analysis (Qiagen).
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bioRxiv - Developmental Biology 2021Quote: ... 250 μl of Protein Precipitation Solution (QIAGEN) was added to each sample and were incubated on ice for 5 min followed by vigorous vortexing for at least 30 sec ...
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bioRxiv - Molecular Biology 2021Quote: ... For protein purification Ni-NTA agarose (Qiagen) was used ...
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bioRxiv - Genomics 2020Quote: ... 333 μL of Protein Precipitation Solution (Qiagen) was added to each sample which was then vortexed and then centrifuged at 2000 x g for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by Ni-NTA (Qiagen) and eluted with 300 mM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: AllPrep DNA/RNA/Protein Mini Kits (Qiagen) were used to extract total proteins from UVA-exposed and non-exposed HTEpC from all experiments ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... For protein purification using NiNTA agarose (Qiagen), cells were lysed in 50 mM phosphate buffer ...
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bioRxiv - Systems Biology 2023Quote: ... and 20 µL Protein Kinase K (Qiagen) for 30 min at RT followed by the steps as described in the protocol of Dneasy Blood & Tissue Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were bound to Ni-NTA (Qiagen), washed with 10 column volumes of Buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were bound to Ni-NTA (Qiagen), washed with 10 column volumes of Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... peritoneal membrane sections (80 mg) were dissociated in 1 ml buffer RLT (QIAGEN) supplemented with β-mercaptoethanol (1:100 v:v ...
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bioRxiv - Molecular Biology 2020Quote: ... Membrane-bound genomic DNA was digested with RNase-Free DNase Set (Qiagen, 79254). First strand cDNA was synthesised using a ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs ...