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Citations for Qiagen :
2101 - 2150 of 2210 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Pathology 2022Quote: ... tissue samples were homogenized in 1 ml of phosphate buffered saline with a metal bead using a TissueLyzer II (Qiagen GmbH). Viral nucleic acids were extracted from blood and homogenized spleen ...
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bioRxiv - Immunology 2022Quote: ... 350 µL of aqueous phase was transferred into a 2 mL reaction tube and subjected to the QIAcube (QIAGEN, Hilden, Germany) for RNA isolation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA extractions were performed on cell pellets from 15 mL of these back-diluted cultures using QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Systems Biology 2024Quote: ... The samples were then cooled for 5 minutes on ice and transferred to a pre-spun 15 mL MaXtract tube (Qiagen 129065) containing an equal volume of chloroform to the acid phenol used ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from OSU-CLL cells (1.5e6 cells/mL) treated with DMSO or SpiD3 (1, 2 μM; 4 h) using the miRNeasy Mini Kit (Qiagen; Hilden, Germany) per manufacturer instructions and processed using the Universal Plus mRNA-Seq with NuQuant kit (Tecan ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from 50 mL wastewater samples within 24h of sampling using the DNeasy® PowerWater Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2024Quote: ... posterior eye cups (PECs) were separated and collected in 2 mL reinforced tubes (SPEX Sample Prep) containing 5 mM stainless steel beads (Qiagen, LLC).
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bioRxiv - Cancer Biology 2023Quote: ... Deproteination of the D-2-HG quantification aliquot was achieved using 3.0 mL of Proteinase K (Qiagen, Catalog #19131, Hildren, Germany) per 100.0 mL of cell lysis solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The samples were then transferred to a 15 ml tube and processed with a Qiagen Midi Prep Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... After protein digestion (PK Buffer and Proteinase K (20 mg/mL) for 2 hrs at 45°C) DNA was purified with QIAquick PCR Purification Kit (Qiagen, Germany) according to manufacturer instruction ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from 200 ml of buffy coat using the Qiagen QIAamp DNA Blood Mini Kit (Qiagen, Cat No. 51106) according to the manufacturer’s instructions and including RNA digestion ...
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bioRxiv - Microbiology 2022Quote: ... The spooled DNA was washed with approximately 5 mL of 70% ethanol and dried before being suspended in 300µL of EB buffer (QIAGEN, Hilden, Germany). Purified DNA was quantified by Qubit (Thermofisher).
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA by adding 3-5 mg of tail tissue to 150 µl of 10% ChelexR resin (Bio- Rad, Inc.) with 1.4 µl of Proteinase K (20 mg/ml, Qiagen, Chatsworth, CA), incubating at 55°C for 180 min ...
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bioRxiv - Cancer Biology 2023Quote: ... medium was removed and cells were lysed on the plate by addition of 3 ml of lysis reagent RLT Plus (Lot 169010027, Qiagen, Germany). 1 ml of lysate was transferred to a 2 ml Eppendorf tube ...
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bioRxiv - Synthetic Biology 2023Quote: ... The recombinant plasmid was then purified at a high concentration (200 μg/mL) using the plasmid preparation kit (Qiagen, Germantown, MD).
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Melted samples (different volumes from 300 to 1000 ml) were filtered on 0,22 μm pore size hydrophilic PVDF filters Durapore® and DNeasy Power Soil Pro® Kit (Qiagen) was applied for DNA extractions as is the most common and efficient method used in microbiome surveys51,67,68 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Systems Biology 2023Quote: ... The spheroblasted cells were collected in 50 mL Falcon tubes and spun for 5 min at 2,500 g and re-suspended in 7.5 mL of home-made buffer P1 (according to the manufacturers recipe, QIAGEN, Düren, Germany). 7.5 mL of home-made buffer P2 (according to the manufacturers recipe ...
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bioRxiv - Physiology 2024Quote: ... 0.5% SDS and 20mg/ml of Proteinase K) and DNA purified using the DNeasy Blood and Tissue Kit (Qiagen, Venlo, Netherlands). PCR was performed with extracted DNA and primers for the recombined and non-recombined alleles as previously described [5] ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 ml blood were collected into EDTA blood collection tubes and processed within 2 h using the AdnaTest (Qiagen, Hilden, Germany). Patients were divided into HSPC ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Cancer Biology 2024Quote: ... additional DNA from 1-5 mL of plasma was extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114). Illumina sequencing libraries were constructed using SRSLY® PicoPlus DNA NGS Library Preparation Kit (ClaretBio – Cat ...
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bioRxiv - Immunology 2024Quote: ... We collected 2.5 ml of CSF and whole blood for transcriptional profiling in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Microbiology 2024Quote: ... using standards previously generated.65 We collected 2.5 ml of CSF and whole blood for transcriptional profiling in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Evolutionary Biology 2020Quote: All genomic DNA was extracted using a Bioline Isolate II Genomic DNA Extraction Kit (Cat#. BIO-52067) following the recommended protocol with an additional DNAse free RNaseA (100mg/ml) (Qiagen cat. #19101) treatment before column purification ...
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bioRxiv - Microbiology 2022Quote: ... Organs were homogenized by high-speed shaking in 2 mL microcentrifuge tubes filled with sterile PBS and 5/7 mm stainless steel beads using TissueLyser LT (Qiagen, Hilden, Germany).
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bioRxiv - Genetics 2020Quote: ... were washed with 5 ml PBS twice and the total RNA was isolated using a RNeasy Mini Kit (74104; Qiagen, Hilden, Germany). The solution was subsequently treated with DNase I (79254 ...
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bioRxiv - Genetics 2019Quote: ... and supernatant was mixed with 10 times its volume of “2M70” binding buffer (2 M guanidine hydrochloride and 70% isopropanol) in a 15 mL tube and passed through QIAquick silica columns (Qiagen, Hilden, Germany) using 25 mL tube extenders (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... cfDNA was extracted from 1 or 4 ml of urine according to manufacturer recommendations (Qiagen Circulating Nucleic Acid Kit, Qiagen, Valencia, CA) and quantified using a Qubit fluorometer 3.0 (high sensitivity double-stranded DNA kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One million cells per well were seeded in 6 well plates in 2 mL growth medium the day before transfection and transfected with PolyFect (Qiagen, Düsseldorf, DE) using the manufacturers protocol (changing the medium to growth medium without hygromycin B and puromycin prior to transfection) ...
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bioRxiv - Immunology 2021Quote: ... The dried DNA pellet was reconstituted in 50 μL H2O treated with 330 μg/mL of RNase A for 2 hours at 37°C and then recovered using the QIAquick PCR Purification kit (QIAGEN Cat#28104). DNA concentration was determined by Qubit (Thermofisher).
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was further treated with 25 µL of proteinase K (20 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19133), gently mixed by flicking of tube and incubated at 56 °C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged proteins were recovered from the clarified lysate using a drip column containing a 5.0 ml bed volume of Ni-NTA resin (Qiagen, Mississauga, ON, Canada). After extensive washing with buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Tissues were homogenized in 1 ml cell culture medium (see above) and a 5 mm steel bead in a TissueLyser (Qiagen, Hilden, Germany). Fecal samples were vortexed in sterile NaCl and the supernatant was sterile filtered (22µm ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 3 ml of each raw milk sample using the PowerFood™ Microbial DNA Isolation Kit (MoBio Laboratories, Qiagen, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... the root and leaf dry matter were ground into powder by placing the samples into glass vials with three opposing blades and shaking at a frequency of 30 Hz for 10 minutes using a Qiagen TissueLyser II (Qiagen, ML, USA). Ground root and leaf percent N was determined by the Dumas method using the Elementar rapid N exceed analyzer (Elementar Americas Inc ...
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Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
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bioRxiv - Microbiology 2022Quote: ... Samples and the DNA extraction negative control (1 ml PBS) were processed for DNA extraction using the QIAamp DNA Microbiome kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Depletion of Host DNA protocol).
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bioRxiv - Microbiology 2022Quote: ... aeruginosa cells were lysed after 18 hours using 2.5mg/mL lysozyme and incubated at 37°C for 30 minutes and RNA was extracted using the RNeasy mini kit (QIAGEN, Germantown, MD). DNase treatment was done twice for better DNA removal using Qiagen RNase-free DNase during RNA extraction and using NEB DNase after RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
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bioRxiv - Cancer Biology 2021Quote: PBMCs were brought up to 15 mL volume in cold PBS and DNA was isolated with the DNeasy Blood & Tissue Kit (Qiagen catalog # 69504) according to the manufacturer’s protocol with 1 hour of incubation at 55°C for digestion ...
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bioRxiv - Immunology 2020Quote: ... 1µg/ml Aprotinin, 1mM PMSF) using 5mm Stainless Beads and TissueLyser II (frequency: 30/sec, duration 2 × 30 sec) (Qiagen, Hilden, Germany). IL-23 was measured using either a mouse IL-23 ELISA (R&D ...
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bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: Reduced His-tagged PRX-IIE (3 mg) or PRX-IIE C146S (3 mg) were bound to 1 mL Ni-NTA resin (Qiagen, Hilden, Germany) and used as affinity matrix ...