Labshake search
Citations for Qiagen :
1851 - 1900 of 2105 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... all the weighted sample tubes were transferred to ice until the samples were thawed and added 1.0 ml of lysis buffer from QIAamp Fast DNA Stool Mini Kit (Cat no. #51604; Qiagen, Germany). All the procedures were followed as recommended by manufacturer’s guidelines for bacterial DNA isolation and DNA was dissolved in 100 μl instead of 200 μl DNA buffer.
-
bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
-
bioRxiv - Microbiology 2019Quote: The DNA samples for shotgun metagenome sequencing were extracted from 50 mL each of the four activated sludge samples using DNeasy PowerSoil Kit (Qiagen). Sequencing of the metagenomic DNA was performed at Macrogen Inc. ...
-
bioRxiv - Immunology 2021Quote: ... The protein complex was adjusted to 14 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
-
bioRxiv - Biochemistry 2019Quote: ... Cell lysate was separated by centrifugation at 28000 x g for 45 min and 4°C and the supernatant was loaded onto a column containing 2 mL of Ni-NTA agarose (Qiagen) equilibrated with buffer A for 1 h for purification by nickel affinity chromatography ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing His-eIF4A1 or His-eIF4A2 was incubated with a 1.5 ml bed volume of Ni-NTA Superflow (Qiagen, 30430) or a 0.75 ml bed volume of Ni-NTA agarose (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... Preparation of total RNA and Northern blotting including the determination of RNA half-lives were carried out as described previously [29] except that 1 ml time samples were taken and directly added to 250 μl RNAprotect Bacteria Reagent (Qiagen) or to 250 μl containing 5 % phenol and 95 % ethanol ...
-
bioRxiv - Microbiology 2020Quote: ... Each complex was adjusted to 13 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) and ProPlex screen (Molecular Dimensions ...
-
bioRxiv - Microbiology 2020Quote: ... and used 1.8 ml of this liquid culture to extract DNA using the DNeasy UltraClean microbial kit (Qiagen, Venlo, Netherlands) following manufacture protocols ...
-
Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
-
bioRxiv - Plant Biology 2019Quote: ... fifty milligrams of fresh leaves in a 2-ml tube was frozen in liquid nitrogen and ground using a Tissuelyser II (Qiagen). The ground sample was extracted with 500 μl of extraction solvent ...
-
bioRxiv - Cancer Biology 2019Quote: Genomic DNA was extracted from up to 1 mL whole blood or buffy coat using the Gentra Puregene Blood Kit (Qiagen) as per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... To the clarified lysate (generally about 50 mg/mL protein content as measured with absorbance at 280 nm) was then added 5 mL of pre-washed (lysis buffer) Ni-NTA resin slurry (Qiagen). The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn) ...
-
bioRxiv - Biochemistry 2021Quote: ... before extraction solution was added to achieve a concentration of 20 mg/mL before immediate lysis in a benchtop TissueLyser LT (Qiagen) operated at 50 Hz for 3 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... The soluble extracts were applied to 2-ml columns of nickel-nitrilotriacetic acid- agarose (Ni-NTA) (QIAGEN catalog no. 30210) that had been equilibrated with lysis buffer without protease inhibitors ...
-
bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
-
bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
-
bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
-
bioRxiv - Genetics 2020Quote: ... 2 ml of peripheral blood was used for germline DNA extraction using the QIAamp DNA Blood midi kit (Qiagen, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... cells were either left untreated or treated with 1000 IU/ml recombinant IFN-α treatment and incubated for 6 h or transfected overnight with polyI:C (10 μg/ml) using Polyfect (Qiagen). Luciferase assays were carried out ...
-
bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 45 min after which 25 µl of proteinase K (20 mg/mL) and 200 µl Buffer AL (Qiagen) was added ...
-
Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Lysates were cleared by centrifugation at 15000 x g for 30 min at 4 °C and incubated in Pierce 5 ml columns with Ni-NTA agarose beads (Qiagen) at 4 °C for 1 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... The specimens were gently and quickly homogenized using a BioMasher II homogenizer (Funakoshi) and mixed with 2 mL of Buffer G2 (QIAGEN), including 200 µg/mL RNase A and 50 µL proteinase K (20 mg/mL) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation at 24,000g at 4°C for 20 min and passed through 2 mL of Ni-NTA nickel resin (Qiagen, 30250) pre-equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3 mm steel ball, 25 Hz for 1 min in a TissueLyser (Qiagen)) ...
-
bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3mm steel ball, 25 Hz for 1 minute in a TissueLyser (Qiagen)) ...
-
bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
-
bioRxiv - Molecular Biology 2020Quote: ... the supernatant of the bacteria lysate was transferred into new 50 ml Falcon tubes and incubated with Ni-NTA (Qiagen) at 4 °C for 30 min ...
-
bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
-
bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
-
bioRxiv - Genomics 2021Quote: ... 1 g of ground leaf powder was resuspended in lysis buffer with 1 mg/ml of proteinase K (19133, Qiagen) and 190 μg/ml of RNase A (19101 ...
-
bioRxiv - Genomics 2020Quote: ... aseptic LS007 shoot tissue was ground under liquid nitrogen using a mortar and pestle and resuspended in 2 mL of AP1 (Qiagen) with 20 µl of RNase I (Qiagen) ...
-
bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
-
bioRxiv - Cancer Biology 2020Quote: ... or 50 um sections (liver metastases) were cut and homogenized in 1 ml of AllPrep DNA/RNA Mini Kit (Qiagen) using a plastic pestle (Cole-Palmer #44468-23) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Isolated colonies were inoculated into 5 mL of LB with appropriate antibiotics and plasmids were purified using the alkaline lysis protocol and Qiaquick miniprep kit (Qiagen). Plasmids were verified by sequencing (GATC ...
-
bioRxiv - Biophysics 2022Quote: ... peak fractions were supplemented with 10 mM imidazole and 10 µg/mL aprotinin/leupeptin and bound to NiNTA beads (Qiagen) for 2 hours ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
-
bioRxiv - Plant Biology 2022Quote: ... the supernatant was discarded before resuspending the nuclei pellet in 30 mL prewarmed (37 C) G2 lysis buffer (Qiagen, Germany). Resuspended pellets were incubated with RNase A (50 μg/ml ...
-
bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
-
bioRxiv - Microbiology 2022Quote: ... The protein complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
-
bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
-
bioRxiv - Microbiology 2022Quote: RNA was isolated from approximately 107 cells grown to a density of 106 cells/ml using the RNAeasy Mini Kit (Qiagen), including an on-column DNase I treatment (Qiagen) ...
-
bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
-
bioRxiv - Microbiology 2022Quote: ... Tissue sections collected for virology were disrupted in 1 mL of sterile PBS using the TissueLyser LT (Qiagen, Germantown, MD) at 30 Hz for 5 min twice ...
-
bioRxiv - Immunology 2022Quote: ... The protein complex was adjusted to 11 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
-
bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
-
bioRxiv - Biophysics 2022Quote: ... The resulting mixture was rotated at 4 °C for 60 min and then poured into a 1 mL polypropylene column (Qiagen). The resin was washed three times with 5 mL lysis/wash buffer and eluted in 0.25 mL fractions with Ni-NTA elution buffer (composition same as lysis/wash buffer ...
-
bioRxiv - Biophysics 2022Quote: ... Cell debris was cleared by centrifugation at 40,000 g for 45 min at 4°C and supernatant was applied to His-tag affinity purification using Ni-NTA agarose (beads or 5 ml column, Qiagen) in buffer A (50 mM TRIS ...