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Citations for Qiagen :
1701 - 1750 of 2105 citations for Potassium Bromate 90 95% Chemical Purity 18O3 98% 100 Ug Ml In 18O Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated in six biological replicates per strain from 0.5 mL of culture using the RNeasy Protect Bacteria Mini kit (Qiagen) on six consecutive days ...
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bioRxiv - Developmental Biology 2020Quote: ... Femoral vein blood collection (1.0-2.0 mL) was performed on each animal and blood was placed in to PAXgene blood collection tubes (Qiagen). Whole blood was frozen and stored at −80° C for shipment to UCLA for methylation analysis.
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bioRxiv - Genetics 2021Quote: ... 10 mM imidazole (final concentration) was added to the sample and the solution was bound to 0.5 ml NiNTA resin (Qiagen). The resin was washed with NTA buffer A1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... cells were first washed and pelleted five times with 15 ml of PBS then lysed with QIAzol reagent (Qiagen), RNA was extracted according to manufactures directions ...
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bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Biochemistry 2021Quote: ... The ankyrin repeat domains were purified over a Ni-nitrilotriacetic acid column (2.5 ml column volume) according to the manufacturer’s instructions (QIAgen, Germany). Up to 200 mg of highly soluble ankyrin repeat domains were purified from one liter of E ...
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bioRxiv - Genetics 2021Quote: ... We extracted DNA from approximately 1-2 million cells of actively growing culture by first pelleting the cells and resuspending them in 1.0 mL Cell Lysis Solution (Qiagen). The samples were incubated with RNase A solution at 37°C for 40 min ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen pieces of tissue (~20 mg) were homogenized in 0.5 ml TRIzol in a TissueLyser II (Qiagen, Hilden, Germany) (2 × 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... or ectodomain buffer (20 mM Tris, pH 8.5, 500 mM NaCl) and applied to 10 ml Ni-NTA-Sepharose (Qiagen) columns ...
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bioRxiv - Microbiology 2021Quote: ... Samples were spun at 5000 x g for 10 minutes and supernatant was discarded before resuspension in 1.5 mL QIAzol reagent (Qiagen). Screw-cap tubes were prepared for each sample by adding ∼250 µL of sterile beads and the QIAzol suspension mixture was added and incubated for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed to a fresh 50 mL conical and further incubated with 2mL of Talon Beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells suspended at 0.8 × 105 cells/ml were transfected with 50 nM siRNA using HiPerfect transfection reagent (Qiagen) according to the manufacturer’s protocol and analysed after 48 h ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from a 0.5 ml aliquot of the culture using the RNAeasy Protect Bacteria Mini Kit (74524, QIAGEN) with on-column DNAse digestion ...
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bioRxiv - Cancer Biology 2022Quote: ... The femurs were placed with knee-end down in a perforated 0.6 ml tube added 80 μl of RNAlater (Qiagen) and inserted in a 1.5 ml centrifuge tube ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Microbiology 2022Quote: ... 1 to 2 mL of culture were mixed with the same volume RNAprotect™ Bacteria Reagent (Qiagen, Hilden, Germany) incubated for 10 min and centrifuged ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma or serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was recovered by centrifugation at 40 000 × g in a JLA25.50 rotor and put through a gravity flow column with 5 ml of NiNTA Agarose (Qiagen). Bound fraction was washed in Buffer B (300 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Immunology 2022Quote: ... All samples were homogenized in PBS (1 mL for cecal content and 0.5 mL for fecal pellets and mLNs) for 2.5 min at 25 Hz in a TissueLyser (Qiagen, Germany). In the case of mLNs ...
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bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Both biopsies were homogenized in 350 mL of Buffer RLT and then extracted using the RNeasy Micro kit (Qiagen) with on-column DNase digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified bacterial lysates from a 1 l culture were bound to a 0.5 ml column of Ni-NTA resin (Qiagen) by gravity flow ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of logarithmic growth phase culture was pelleted by centrifugation and resuspended by vortexing in B1 buffer (Qiagen, Hilden Germany ...
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bioRxiv - Neuroscience 2023Quote: ... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and its genomic DNA was amplified in the same 0.2-mL PCR tube using the REPLI-g single-cell WGA kit (QIAGEN) to obtain single-worm WGS data from LJ2050 ...
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bioRxiv - Developmental Biology 2023Quote: Blastocysts were washed with DPBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by incubation with 6 ml of lysis buffer (50 mM Tris pH 8.0, 50 mM EDTA, 1% SDS, 0.1 mg/ml proteinase K (Qiagen, 19131)) at 55°C for 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were moved on magnet and supernatant was transferred to clean 1.5 mL tubes for nucleic acid extraction using the MinElute PCR Purification Kit (Qiagen). Purified DNA was used for library preparation using the NEBNext Ultra Library prep Kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by three washes with 2X SSC at 37°C and treatment with RNaseA (200ng/ml; Qiagen) in 2X SSC at 37°C ...
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bioRxiv - Microbiology 2023Quote: The supernatant was collected and at room temperature was passed over a 2.0 mL bed volume of Ni-NTA Sepharose resin (Qiagen). The column was then washed three times with 10.0 mL of wash buffer (50.0 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was centrifuged at 15,000 x g for 35 mins and the soluble fraction incubated with 3 mL of Ni-NTA beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Deparaffinization with Xylene and RNA extraction was performed in sterile 1.5 ml microcentrifuge tubes with the RNeasy FFPE Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was 0.2 µM filtered and was incubated overnight at 4°C with 5 mL of Ni-NTA beads (Qiagen). XXX
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1 mg/mL Lysostaphin (MilliporeSigma) was added in the pre-treatment step in combination with enzymatic lysis buffer (Qiagen). Bacterial strains are listed in Table S2.
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1 mg/mL Lysostaphin (MilliporeSigma) was added in the pre-treatment step in combination with enzymatic lysis buffer (Qiagen). Purified DNA was stored at −20°C before further processing for NGS or cell-based detection.
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the supernatant incubated 1 hr at 4°C with 1.5 ml packed Ni-NTA Agarose beads (Qiagen). Beads were washed with buffer H adjusted to 50 mM imidazole ...