Labshake search
Citations for Qiagen :
2001 - 2050 of 2378 citations for 6H 5 Oxa 1 2a 4a triazacyclopenta cd pentalene 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
-
bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
-
bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Immunology 2020Quote: ... mRNAs recovered from microdissected samples or vaginal biopsies (about 1 mm3) were reverse-transcribed with the QuantiTect Rev Transcription Kit (Qiagen), used according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
-
bioRxiv - Microbiology 2020Quote: ... Preparation of total RNA and Northern blotting including the determination of RNA half-lives were carried out as described previously [29] except that 1 ml time samples were taken and directly added to 250 μl RNAprotect Bacteria Reagent (Qiagen) or to 250 μl containing 5 % phenol and 95 % ethanol ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
-
bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
-
Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
-
bioRxiv - Bioengineering 2021Quote: ... for 1 day prior to extraction of total RNA of the treated leaves with an RNeasy plant mini kit (QIAGEN). Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves ...
-
bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
-
bioRxiv - Genomics 2021Quote: ... The PCR products were size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The pSTARR-seq_human vector (kindly provided by Alexander Stark ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue microregions were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
-
bioRxiv - Immunology 2021Quote: ... A total of 1-2 μg of RNA was used to synthesize the first single-strand cDNA using QuantiTect Reverse Transcription kit (Qiagen). For RT-PCR amplification ...
-
bioRxiv - Immunology 2020Quote: RNA was extracted from isolated neutrophils after the 1 and 4 hour EC challenges as well untreated controls using the miRNeasy Micro kit from Qiagen and libraries were generated using KAPA PolyA enrichment mRNA library prep ...
-
bioRxiv - Biophysics 2019Quote: ... Insoluble material was pelleted for 1 hr at 35,000 rpm and supernatant was added to Ni-NTA resin (QIAGEN, 30410) in batch and rotated at 4°C for 1 hr ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were electrophoresed in a 1% agarose gel and the gel bands were isolated using QIA Quick PCR Purification Kit (Qiagen). Isolated DNA was cloned into pCR-TOPO plasmids using the TOPO TA cloning kit (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2019Quote: Genomic DNA was extracted from up to 1 mL whole blood or buffy coat using the Gentra Puregene Blood Kit (Qiagen) as per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... an 8 μg of the isolated RNA was further treated with 2U of TURBO DNase (Invitorgen) at 37°C for 1 h and purified with RNeasy spin columns (Qiagen). The amount of RNA was quantified with Qubit RNA BR assay kit (Invitrogen) ...
-
bioRxiv - Biochemistry 2019Quote: ... The template was run on a 1% agarose gel and extracted using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). DNA template concentration was determined by a Qubit 3.0 Fluorometer (Life Technologies ...
-
bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
-
bioRxiv - Genomics 2021Quote: ... To eliminate lipids supernatants were applied to a RNeasy column and centrifuged at 10,000 rpm and 4 °C for 1 min (Qiagen, 74104). The flow through was collected and protein concentrations were assessed using the Bradford assay ...
-
bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
-
bioRxiv - Biochemistry 2021Quote: pAC5.1 vectors with CLIP-CRY and TIM-SNAP-HA were delivered into s2 cells using Effectene reagent (Cat# 301425, Qiagen) or TransIT-Insect Transfection Reagent (Cat# MIR6100 ...
-
bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2021Quote: ... The LNA gapmers for lnc-FLii-1 and lnc-LSAMP-3 were designed and purchased along with negative control LNA from Qiagen. For transfection ...
-
bioRxiv - Cancer Biology 2021Quote: The frozen tumors were minced to small pieces and suspended in buffer P1 (QIAGEN, 1 mL Buffer/100 mg tumor) supplemented with 100 μg/mL RNase A (Promega) ...
-
bioRxiv - Genomics 2022Quote: ... The indexed PCR products were cleaned using 1 Volume AMPure XP beads on magnetic stand and eluted with EB buffer (QIAGEN) followed by quantification using a Qubit dsDNA HS Assay Kit ...
-
bioRxiv - Developmental Biology 2021Quote: ... Skin samples were taken by remote biopsy dart (1 mm wide by 20-25 mm deep plug) and preserved in RNAlater (Qiagen). Blood samples were taken opportunistically during veterinarian visits and preserved in EDTA tubes ...
-
bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
-
bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
-
bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
-
bioRxiv - Genomics 2021Quote: We began the assembly by first mapping long reads to the S288c reference genome (version R64-2-1) using CLC Genomics Workbench (Qiagen)(Fig ...
-
bioRxiv - Immunology 2020Quote: RNA was isolated from individual pools of 1×104 to 2×104 double-sorted SLAM cells using an RNEasy Micro kit (Qiagen). RNA was quantified and quality checked using an Agilent Bioanalyzer 2100 (Agilent) ...
-
bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
-
bioRxiv - Molecular Biology 2021Quote: ... for a total of 6 min (2 x 3 min with 1 min on ice in between homogenisations) at 50 Hz using a TissueLyser LT (Qiagen). Thereafter ...
-
bioRxiv - Molecular Biology 2020Quote: ... 50uL of the dounce homogenized sample was labelled as “input” and added to 350uL RLT buffer with 1% 2-mercaptoethanol (Qiagen), followed by RNA extraction ...
-
bioRxiv - Immunology 2021Quote: Heat-inactivated human serum was diluted 1:10 in phosphate buffered saline (PBS) and applied to a gravity polypropylene flow column (Qiagen) containing 1 mL of Protein G-sepharose resin (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
-
bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
-
bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). A first round of PCR was performed using primers GSP1 and P1 (First denaturation step ...
-
bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
-
bioRxiv - Biochemistry 2021Quote: ... and early stationary phase (OD = 1.0-1.3).1 mL sample of each culture was directly transferred to 2 mL RNAprotect cell reagent (Qiagen, Hilden, Germany). The samples were vortexed 5 sec ...