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Citations for Qiagen :
1801 - 1850 of 2378 citations for 6H 5 Oxa 1 2a 4a triazacyclopenta cd pentalene 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Genomics 2022Quote: ... PCR#1 amplicons were selected on a 2% agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen). These amplicons were then used as template for “PCR#2” reactions ...
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bioRxiv - Physiology 2022Quote: Frozen liver tissue was manually crushed and 30 mg per sample was homogenized in 1mL of 2:1 chloroform:methanol via a TissueLyser II (Qiagen). Samples were stored at 4 °C overnight with agitation ...
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bioRxiv - Genetics 2022Quote: ... The lysates were cleared by centrifugation at 10,000 xg for 30 min and added to 1 ml of Ni-NTA agarose (Qiagen) pre-equilibrated with the same lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... tissue was digested away from the slide by incubating the tissue with 1% 2-mercaptoethanol in RLT buffer (Qiagen) for one hour at 56°C with interval shaking ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products of the expected size were excised from a 1 % w/v agarose gel and purified using the QIAquick gel extraction kit according to the manufacturer’s protocol (Qiagen). The cDNA fragment was inserted into the pCR4-TOPO® vector and transformed into OneShot® chemically competent E ...
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bioRxiv - Microbiology 2019Quote: A total of 250 ng of Qst was incubated with 1 ml Ni-NTA Superflow beads (Qiagen, Hilden, DE) in an end-over-end shaker for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... or lungs (pneumonia) were harvested and homogenized in 1 mL PBS + 0.1% Triton-X100 using a TissueLyser LT (Qiagen) before plating to LB agar + 5 μg/ml irgasan to enumerate bacterial burden.
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bioRxiv - Microbiology 2020Quote: ... adjusted to pH 8.0 using 1M Tris-HCl pH 8.0 and incubated at room temp for 1 h with Ni-NTA Agarose beads (Qiagen). Beads were washed twice with PBS ...
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Genetics 2019Quote: ... mixed with 1 volume of 70% ethanol and then transferred to a RNeasy MinElute column (Qiagen, Hilden, Germany: 74204) for purification following the standard protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue was digested away from the slide by incubating the tissue with 1% 2-mercaptoethanol in RLT buffer (Qiagen) for one hour at 56°C with interval shaking ...
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bioRxiv - Systems Biology 2020Quote: DNA was isolated from CAMA-1 and CAMA-1_ribociclib_resistant cells using DNeasy Blood & Tissue Kit (Qiagen, Cat. No.: 69504) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Physiology 2021Quote: ... feces were homogenized in 1 mL 80% (v/v) methanol using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Five days later 1×107 cells were harvested and DNA isolated for sequencing (QIAamp DNA Blood Midi Kit, Qiagen). For each experimental condition (mock ...
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bioRxiv - Genetics 2020Quote: ... The PCR product was separated on a 1 % agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen). 60 ng of each probe were labeled with dATP [α-32P] (DECAprime kit II ...
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bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from mantle or arm tissue (~1 mm3) using a DNeasy Blood and Tissue Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The amplified genes were then electrophoresed in 1% agarose gel followed by purification using PCR purification kit (Qiagen, USA). Both purified genes and plasmids were digested with Nde I and Not I prior to ligation using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was purified from THP-1 cells using the QIAmp DNA mini and blood extraction kit (QIAgen, Manchester, UK) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Genomics 2021Quote: ... and an aliquot of 300-500uL was immediately mixed at 1:2.76 volume ratio with fluid from a Paxgene blood RNA tube (Qiagen), whilst the remainder was stored on ice for flow cytometry analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation for 1 h at 4°C at 10,000 rcf before being subjected to Ni-NTA (Qiagen) affinity purification following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor, 2.5 μM template switch oligo with Qiagen #339414YCO0076714 ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from DF-1 cells and HH27 whole chick heads using the RNeasy Plus Kit (Qiagen, 74136) following the manufacturer’s directions ...
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bioRxiv - Cell Biology 2020Quote: ... and A10 encoding constructs or 1 μg Shh constructs with or without 0.5 μg Scube2 by using Polyfect (Qiagen). Cells were grown for 2 days or 3 days for Disp−/− rescue experiments at 37°C with 5% CO2 in DMEM containing 10% fetal calf serum and penicillin-streptomycin (100 μg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were analyzed on a 1% agarose gel and bands gel purified using QIAquick gel extraction kit (Qiagen). Samples were sequenced via Sanger sequencing (Genewiz ...
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bioRxiv - Molecular Biology 2020Quote: ... 15uL of the sample was de-crosslinked overnight at 65°C with 85ul of decrosslinking buffer (0.1M NaHCO3, 1% SDS) before clean up using QiaQuick PCR clean up kit from Qiagen.
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was isolated following the manufacturer’s protocol and cDNA was synthesized from 1 µg total RNA using the QuantiTekt Reverse Transcription kit (Qiagen). The primers used for detecting Ccdc186 cDNA were:
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bioRxiv - Genomics 2021Quote: ... Illumina reads for each genome were mapped to the yeast reference genome (R64-2-1, yeastgenome.org) using CLC-Genomics software (Qiagen). Resulting read mapping files were then subjected to copy number and heterozygous single nucleotide polymorphism (hetSNP ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Immunology 2022Quote: ... The complementary DNA (cDNA) was synthesized from 1 μg of total RNA using QuantiTect Reverse Transcription Kit (Qiagen: 205311) and quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of extracted RNA from each sample was used for the cDNA synthesis (cDNA synthesis kit, Qiagen, Germany) according to the suppliers’ protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared from 1 μg total RNA using the QIAseq FastSelect -rRNA Yeast kit (Qiagen 334217) and QIAseq Stranded RNA Library kit (Qiagen 180743 ...
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bioRxiv - Genomics 2022Quote: ... Illumina reads for each genome were mapped to the yeast reference genome (R64-2-1, yeastgenome.org) using CLC-Genomics software (Qiagen). Resulting read mapping files were then subjected to copy number and heterozygous single nucleotide polymorphism (hetSNP ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 µg of each sample was used to prepare 20 µL cDNA using RNeasy Plus Mini Kit (Qiagen). Human RT2 Profiler™ PCR Array ...
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bioRxiv - Microbiology 2022Quote: ... 1 to 2 mL of culture were mixed with the same volume RNAprotect™ Bacteria Reagent (Qiagen, Hilden, Germany) incubated for 10 min and centrifuged ...
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bioRxiv - Physiology 2022Quote: ... Lysates were centrifuged at 100,000 x g for 1 h and the supernatant were affinity purified on Ni-NTA resin (Qiagen) by batch binding for 30 min-1h ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Microbiology 2022Quote: Airway epithelium cultured on 6.5-mm Transwell membranes was washed and treated for 1 min at RT with RLT buffer (Qiagen) with 0.01 % β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The lysate was centrifuged at 50,000×g for 10 min and to the supernatant 1 mL of 50% Ni−NTA (Qiagen) was added and incubated for 1 h at 4 °C ...