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Citations for Qiagen :
2251 - 2300 of 2378 citations for 6H 5 Oxa 1 2a 4a triazacyclopenta cd pentalene 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was cut from a 1 % agarose gel and purified using the QIAGEN PCR purification kit (QIAGEN, Hilden, Germany). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... The digested vectors were run on 1% agarose gel and the target band was cut and harvested using QIAquick Gel Extraction Kit (QIAGEN 12362). The gel extraction products were further purified with QIAGEN MinElute PCR purification kit (QIAGEN 28004) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581). 20ng of total DNA was used for qPCR using primers for the KSHV gene ORF57 ...
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bioRxiv - Molecular Biology 2024Quote: ... NEM was subsequently aspirated and the tissue homogenized for 1 min at 30 Hz using a TissueLyser II bead mill (QIAGEN, USA) in ice-cold homogenization buffer [10% glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Bioengineering 2024Quote: ... IVD cells that were attached to the well were washed with 1% dPBS and lysed using RLT buffer (Qiagen, Hilden, Germany) and 1% mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... The digest was run on an 1% Agarose gel (containing ethidium bromide) and the pLV6 backbone was extracted with the QIAquick Gel extraction kit (Qiagen 28704) according to the manufacturer’s manual.
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the RNA QuantiTect transcription kit (Qiagen, Cat # 205311). Approximately 100 ng of cDNA was used as a template for each qRT-PCR reaction using the PowerTrack SYBR Green supermix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... was run in a 1% agarose gel and the desired band (1.8 kb) was purified using QIAquick gel extraction kit (Qiagen, Cat. No. 28704) and used as a template for the transcription reaction ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from the supernatant of cells infected with Candid #1 (P0 and P11) using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
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bioRxiv - Developmental Biology 2020Quote: ... Japan) and 1 μL of the cDNA synthesis reaction mixture was used with the Platinum SYBR Green kit from Qiagen (Hilden, Germany). q-PCR was performed using a Rotor-Gene Q Real-time PCR machine (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were resolved on a 1% w/v agarose gel and purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and damaged OA cartilage with a Mankin score of 4 or higher (n=1, female, 80 years old) using MiRNeasy Kit (Qiagen, Germantown, USA). RNA was quantified using the Nanodrop 1000 Spectrophotometer (Thermo Fisher ...
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bioRxiv - Zoology 2020Quote: Adult Spadella cephaloptera were starved for three days and sacrificed together with developmental stages covering early zygotes to hatched 1 months old juveniles for RNA extraction by using a RNA extraction kit (Qiagen, Roermond, Netherlands). Additional adults and developmental stages were carefully anesthetized in 7.14% MgCl2 before fixation and fixed for 1 hour at room temperature for in situ hybridization experiments and treated as previously described (Wollesen et al ...
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bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
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bioRxiv - Genomics 2019Quote: ... cfDNA was extracted from 1 or 4 ml of urine according to manufacturer recommendations (Qiagen Circulating Nucleic Acid Kit, Qiagen, Valencia, CA) and quantified using a Qubit fluorometer 3.0 (high sensitivity double-stranded DNA kit ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were blocked with 5% milk in PBST (Phosphate buffer saline, 0.05% Tween 20) and incubated with monoclonal mouse anti-His antibody (Penta His, Qiagen, dilution 1:1000) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Compost was crushed using mortar and pestle under liquid nitrogen and ground to a fine powder for 1 min at 30 Hz using a tissuelyser II with a steel grinding jar pre-cooled with liquid nitrogen (Qiagen, Venlo, Netherlands). RNA was extracted using a modified method described by Patyshakuliyeva et al ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of strains IMX2300 and IMX2300-1 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was extracted from 1-to 2-mm-long tail tips using the DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany). Genomic DNA (5 μl ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Samples and the DNA extraction negative control (1 ml PBS) were processed for DNA extraction using the QIAamp DNA Microbiome kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Depletion of Host DNA protocol).
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour at room temperature either with a commercial blocking buffer (QIAGEN, anti RGS HIS6 HRP conjugate kit) or with 5% milk in 0.05% tween TBS buffer ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Each primer (3.5 μl) was combined with reverse-transcriptase reagent (1 μl) and SYBR® Green master mix (25 μl) (Qiagen, Valencia, CA). The thermal profile was as follows ...
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bioRxiv - Microbiology 2020Quote: ... 120 mg l-1 sodium pyruvate and 10% FCS (supplemented with 10% Fetal Calf Serum and 1% penicillin–streptomycin) at 300 Hz for two minutes using the Tissuelyser II (Qiagen, Hilden, Germany) and centrifuged to clarify the supernatant ...
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bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
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bioRxiv - Molecular Biology 2019Quote: DNA was purified from the eluate and from 1% and 10% input chromatin using QIA-Quick DNA purification kit (Qiagen, Hilden, Germany) and eluted within 50 μl of ddH2O ...
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bioRxiv - Molecular Biology 2019Quote: ... a sample from each tested swatch was collected using a 4N6 FLOQSwab® (Copan Diagnostics) and DNA extracted using a Qiagen EZ-1 instrument (Qiagen, Netherlands). One side of a 4N6 FLOQSwab was wetted with 2 μl of molecular biology grade water and rubbed over the surface of the swatch ...
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bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All samples were diluted to 1 µg of total RNA and were used to synthesize cDNA using a QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) in accordance with manufacturer protocols ...
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bioRxiv - Plant Biology 2020Quote: Reduced His-tagged PRX-IIE (3 mg) or PRX-IIE C146S (3 mg) were bound to 1 mL Ni-NTA resin (Qiagen, Hilden, Germany) and used as affinity matrix ...
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bioRxiv - Bioengineering 2020Quote: ... pelleted and lysed (Qiagen RLT lysis buffer supplemented with 1% β-Mercaptoethanol. RNA was recovered using spin columns (Qiagen MicroRNAeasy® kit), with on-column DNAse I digest performed according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For the detection of phosphorylated Serine residues a TBS solution with a 1:100 dilution of the Anti-Phospho-Serin Antibody (Qiagen, Hilden, Germany) was used ...
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bioRxiv - Cell Biology 2020Quote: ... The qPCR was performed using the TaqMan™ RNA-to-CT™ 1-Step kit (Thermo Fischer Scientific) and was run in a RotorGene-6000-2-plex (Qiagen). PCR conditions having reverse transcription at 48’C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from the liver of an adult female Limnodynastes dumerilii dumerilii (Fig. 1) using the Gentra Puregene Tissue Kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions with the following exceptions ...
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bioRxiv - Genetics 2020Quote: ... at least ∼1 × 107 cells were collected by centrifugation and RNA was purified using an RNeasy Mini Kit (Qiagen 74104; Hilden, Germany), including on-column DNase digestion (Cat No ...
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bioRxiv - Microbiology 2020Quote: High-quality genomic DNA was obtained starting from 250 mg of each sample of table 1 using DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... of soluble SARS-CoV-2 WA1 and SARS-CoV-2 Omicron BA.1 spike trimers were isolated from cell supernatants using a Ni-NTA column (Qiagen, Hilden, Germany). Eluents from Ni-NTA purifications were subjected to SEC using a HiLoad Superdex 200 16/600 column followed by a Superose 6 10/300 (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from up to 1 gram of sediment using a modified version of the Qiagen DNeasy PowerSoil kit (Qiagen, Venlo, Netherlands), as described previously [41 ...