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Citations for Qiagen :
1951 - 2000 of 3116 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 1 µg of RNA per sample according to manufacturer’s instructions (Omniscript RT kit, Qiagen). Taqman assay probes (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... there were 1×106 ESCs culturing in 2iLIF condition for genomic isolation with QIAamp DNA Mini Kit (Qiagen). 500 ng of gDNA was used for bisulfite conversion followed by the instructions of EZ DNA Methylation-Gold Kit (Zymo) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... Roots were washed quickly (< 1 min) to detach the surrounding peat matrix using 10% RNAlater (QIAGEN, Valencia, CA) in 1X PBS as the capture buffer to mitigate RNA degradation ...
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bioRxiv - Cell Biology 2020Quote: ... collected in 1 mM EDTA in PBS and total RNAs were isolated using RNeasy Mini Kit (Qiagen, 74106) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... We then washed beads four times in 1 ml of Modified RLT buffer (RLT buffer supplied by Qiagen with added 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: Up to 1×106 culture cells were collected in 350 μl of RLT buffer (QIAGEN RNeasy Mini Kit). RNA was extracted following the QIAGEN RNeasy manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... see Supplemental Table 1 for more details) using either: PN3 ovaries - QIAzol Lysis Reagent (Qiagen, Chadstone, VIC, Australia) and Glycoblue coprecipitant (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR amplifications were carried out using Power SYBR Green RNA-to-CT 1 step kit (Qiagen, UK) on a 384 well ViiA Real-Time PCR System (Applied Bio-systems ...
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bioRxiv - Biochemistry 2021Quote: Total RNAs were extracted from wild-type or DACR HAP-1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of TIE-1 was isolated using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA, USA) and used as a template for PCR reactions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was prepared from 1 μg of total RNA using the QuantiTect RT Kit (Qiagen, Venlo, the Netherlands). Quantitative RT-PCR was performed using TB Green Premix ExTaq II (Takarabio ...
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bioRxiv - Genomics 2021Quote: ... The reaction was stopped by adding 1 μL 0.5 M EDTA (pH = 8.0) then cleaned up with MinElute PCR Purification Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2022Quote: ... mycelia were harvested by filtering and immediately homogenized for 1 min at 30 Hz using a TissueLyzer (Qiagen). Then ...
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bioRxiv - Microbiology 2022Quote: ... and sgRNA-specific and universal primers (Table 1) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen). sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from 1 ml of cells from each culture using a miRNeasy kit (Qiagen, Germany) with some modifications ...
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bioRxiv - Systems Biology 2022Quote: ... siRNA was transfected into RWPE-1 cells in a 24-well plate using HiPerFect Transfection Reagent (Qiagen, 301704). siRNA ...
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bioRxiv - Microbiology 2022Quote: Total community DNA was extracted from 1 g of crushed rocks using DNeasy PowerSoil Pro Kit (Qiagen, German), quality checked by electrophoresis using a 1.5% agarose gel and Nanodrop spectrophotometer (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription reactions were performed with 1 μg of total RNA with the QuantiTect Reverse Transcription kit (Qiagen). Gene expression was measured using TaqMan™ master mix (Thermofisher ...
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bioRxiv - Physiology 2024Quote: ... using 350 microliter RLT buffer with 1% b-mercaptoethanol as a collection/lysis buffer (RNeasy micro kit, Qiagen). RNA was extracted following manufacturer’s instructions (RNeasy micro kit ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded using a peristaltic pump in 1 mL (bed volume) of Ni-NTA agarose (QIAGEN) packed in a Poly-Prep® Chromatography Column (Cat #731-1550 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from frozen Hepa 1-6 cell pellets using the RNeasy Mini kit (74106, QIAGEN) following the manufacturer’s instructions with an on-column DNase digestion step (79254 ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed with 1 µg of template RNA using the Qiagen QuantiTect reverse transcription kit (Qiagen) in a Bio-Rad T100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was then purified from 500,000 to 1 million cells using the miRNeasy Micro kit (Qiagen GmbH) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a minimum of 1 x106 cells per experimental group were processed using the RNeasy Mini kit (#74104; QIAGEN). The mRNA levels were measured with the NanoDrop (DS-11 ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2023Quote: ... 1 μg total RNA was subjected to reverse transcription with a QuantiTect reverse transcription kit (Qiagen, Germantown, MD). The resulting cDNA and Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were washed with 1× PBS and RNA were extracted using the RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from 1 x 106 transduced HEK293T cells with the RNeasy Plus Mini Kit (Qiagen, #74134) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μL of bisulfite treated DNA was amplified for the regions of interest using PyroMark PCR kit (Qiagen) with final primer concentration of 0.4 μM ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by phenol:chloroform:isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR Purification (QIAGEN, Germany). Purified DNA was then analyzed on a 1.5% agarose gel and on Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 cm of the mouse distal ileum was cut into small pieces and stored in RNAlater (Qiagen). RNA was prepared using a combination of Trizol (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then disrupted by sonication and the supernatant was mixed with 1 mL Ni-NTA agarose (QIAGEN) and incubated for 1 h at 4 °C with gentle shaking ...
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bioRxiv - Immunology 2023Quote: ... Total DNA was obtained from the lysed inoculated THP-1 macrophages using DNeasy Blood & Tissue Kit (Qiagen, Germany). The amplification reaction was performed in a 20μl final volume containing 1μl of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of culture was withdrawn and chromosomal DNA extracted using the Dneasy blood and tissue kit (Qiagen). The samples were normalized according to DNA concentrations and diluted 1:5 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen). qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Systems Biology 2023Quote: ... Amplicons were gel-purifed from a 1 % Agarose gel using the QIAquick Gel Extraction Kit (QIAGEN, Düren, Germany). After determining the concentration with Qubit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA extraction was carried out using one of the following methods: 1) QIAmp DNA Mini Kit (Qiagen, Germany); 2 ...
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bioRxiv - Microbiology 2023Quote: ... All treatment groups were supplemented with 1 % FCS and RNA was extracted in RNAeasy Plus lysis buffer (QIAGEN) 6 h post treatment.
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was isolated from 1 mL of overnight culture of each genotype (DNeasy Blood & Tissue Kit; Qiagen). Paired-end ...
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bioRxiv - Cell Biology 2023Quote: ... cells (1 x 106 cells/well) were washed three times with PBS and lysed in Buffer RLT (Qiagen). Lysates were transferred to Eppendorf tubes on ice and stored at -80°C until ready for RNA purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 1 ug of RNA was converted into cDNA using either the Quantitect Reverse Transcription kit (QIAGEN) or the Superscript IV transcription kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... V6.5 mESCs and K562 genomic DNA was purified from 1 million cells using the QIAamp micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1 x 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...