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Citations for Qiagen :
2201 - 2250 of 3116 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1X106 cells were mixed with 5 μl of 20 μM siRNAs (GeneSolution siRNA, Qiagen) and 100 μl of Ingenio electroporation solution (Mirus ...
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bioRxiv - Immunology 2023Quote: Total mRNA was isolated from 5×105 cells using a kit (RNeasy kit, QIAGEN) and reverse-transcribed using oligo(dT)20 primer (SuperscriptIII ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin for 90 minutes stirring in a beaker at 4ºC ...
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bioRxiv - Immunology 2022Quote: ... were isolated using the “miRNeasy Mini Kit” (cell numbers ≥ 5 × 105, Qiagen, Cat# 217004). FACS-isolated FO or MZ B cells were directly sorted into the Qiazol Lysis Reagent (700 μl final volume) ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from pools of 5 females using an RNeasy Mini kit (Qiagen) by first homogenizing them using a plastic pestle in 600 µL of lysis buffer in a 1.5 mL microfuge tube ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Plant Biology 2022Quote: ... The suspension was applied to a column (5 ml polypropylene column, Qiagen, Hilden, Germany) with subsequent washing (5 column volumes ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 5 million sorted bacteria using DNeasy Blood & Tissue (Qiagen, 69504) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Each sample was analysed in duplicate ...
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bioRxiv - Cell Biology 2023Quote: ... The control cells were replated after 5 days of AllStars negative-control siRNA (QIAGEN) treatment ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was loading onto 5 mL Ni-NTA resin (Qiagen, catalog number 30230) equilibrated with buffer A (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... frozen samples were thawed in the presence of 5 volumes of TRI-reagent (Qiagen), pipette mixed thoroughly then centrifuged at 16,000 x g for 1 min ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 minutes with a 5 mm stainless steel ball (Qiagen, Cat. No. 69989). RNA was isolated using the Qiagen RNeasy Lipid Tissue Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 5 g of faecal sample were resuspended in 25 mL of InhibitEx Buffer (Qiagen) and aliquoted into 2 mL tubes containing a mixture of homogenization beads (Thistle Scientific/Biospec Products) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared supernatant was loaded onto a 5 mL Strep-Tactin Superflow Cartridge (Qiagen) at 1 mL/min ...
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bioRxiv - Bioengineering 2024Quote: ... CHMP6 (Hs_CHMP6_1) target sequence: 5′-CTG AGC GCA ATC ACT CAG GAA -3′ (QIAGEN Sciences LLC ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was then mixed with 5 mL of nickel-NTA agarose resin (Qiagen) equilibrated with the lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was subsequently applied to a 5 mL Ni-NTA affinity column (QIAGEN) via gravity flow ...
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bioRxiv - Synthetic Biology 2024Quote: ... Freeze dried samples were turned into powder using a steel bead (5 mm; Qiagen) and the TissueLyser LT (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... We immediately transferred all swabs and film to a microcentrifuge tube with 1 mL lysis buffer and garnet homogenization beads (Qiagen NV ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µM A740003 or both BzATP and A740003 together (A740003 was pre incubated for 1 h before adding BzATP) 24 hours later RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysate [1μg/μL] was RNase A digested at 37°C for 15 min following addition of 1 μg RNase A (Qiagen) per 25 μg protein ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was extracted from 1 B6 and two Card19lxcn BMDMs derived from three independent mice using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: CIRCLE-seq libraries were prepared for each gRNA (IDT) using genomic DNA extracted from NHGRI-1 (DNA Blood & Tissue kit, Qiagen) following the described protocol [59] ...
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bioRxiv - Microbiology 2020Quote: ... We sampled 1 ml of the clear phase for living planktonic cells and extracted DNA using DNeasy Blood & Tissue Kit (Qiagen). The cultures were also plated on SHIEH-agar plates with dilutions for cfu/ml -estimation and colony-PCR.
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Genomics 2020Quote: PCR amplicons verified by 1% agarose gel electrophoresis were purified from gel using Gel extraction kit (Qiagen GmbH, Hilden, Germany) and ligated into a pGEM-T easy cloning vector (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from cavin1a/1b DKO and WT zebrafish embryos (> 100 embryos randomly selected from 1 clutch) using the RNeasy Mini Kit (QIAGEN) and cDNA synthesis was performed using SuperscriptIII reverse transcriptase (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was isolated from 1×106 BMMCs that were untreated or treated with IgE receptor crosslinking using the RNeasy mini kit (Qiagen). RNA was treated with DNase I according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids containing qPCR amplicon sequences were linearized by digestion with SmaI for 1 h and purified using the QIAquick PCR purification kit (Qiagen). Linearized plasmids were quantified using spectrophotometry ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue disruption and homogenization was performed in RIPA buffer supplemented with 1:100 protease using Tissue Lyser LT (Qiagen), for 5 min at 50 Hz ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Bioengineering 2020Quote: Around 250 mg of each produce sample was cut into 1-3 mm pieces using a scalpel and then processed with the DNeasy Powersoil Pro kit (Qiagen) to isolate 50μL of eluted DNA ...
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bioRxiv - Genetics 2021Quote: Amplicons spanning the gRNA target sites were amplified from 1 µL of purified gDNA using HotStar PCR Master Mix (Qiagen). Half of each reaction was run on a 1.5% agarose gel ...
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bioRxiv - Genetics 2020Quote: Bone marrow was isolated by flushing and red cell lysis performed by resuspending pellet in 1 ml of Buffer EL (Qiagen) and incubating on ice for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... The PCR products were subjected to 1 % agarose gel electrophoresis for analysis and purified with QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
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bioRxiv - Cell Biology 2021Quote: RNA samples of MS-5 cells cultured alone and in co-culture with SKM-1 cells extracted using Trizol were purified using the Rneasy Plus Micro kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous Mecp2 cDNA was amplified for analysis using a forward primer in the 5’ untranslated region and a reverse primer located in the 3’ untranslated region PCR products were fractionated on a 1% agarose gel and purified using the QIAquick gel extraction kit (Qiagen) before being submitted for Sanger sequence analysis ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted by two different approaches: 1) for RNASeq: RNA was extracted using a column based kit from QIAGEN GmbH (Plant RNeasy ...
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bioRxiv - Plant Biology 2021Quote: ... The translation mixture was gently agitated in a microtube for 1 h at 4°C with a fivefold volume of Ni-NTA agarose (Qiagen) that had been equilibrated with a solution containing 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Microbiology 2020Quote: Samples for RNA-sequencing were crushed in liquid nitrogen using a sterile pestle and mortar on dry ice and resuspended by vortexing in 1 mL RLT Buffer (Qiagen) supplemented with β-mercaptoethanol (10 μl in every 1 ml buffer) ...
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bioRxiv - Microbiology 2020Quote: ... frozen tissue was partially thawed and submerged in RLT lysis buffer containing 1% ß-mercaptoethanol and 0.5% reagent DX before being homogenized using a hand-operated TissueRupture (Qiagen). To pool samples in equal proportions ...
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bioRxiv - Systems Biology 2020Quote: ... and RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo with a readout probe binding DNA sequence (Qiagen) were hybridized in 50% hybridization buffer consisted of 50% formamide (Invitrogen AM9342) ...
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bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
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bioRxiv - Microbiology 2020Quote: ... frozen tissue was partially thawed and submerged in lysis buffer containing 1% ß-mercaptoethanol and 0.5% Reagent DX before tissues were homogenized together with TissueRupture (Qiagen). The homogenate was centrifuged to remove any potential tissue residues ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was purified 1-3 dpi from mock- or POWV-infected hBMECs or hPCs using RLT lysis buffer and RNeasy columns (Qiagen). Purified RNA was quantitated ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 1 mL digestate slurry or 0.25 g soil using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) following a modified kit protocol where bead beating for 30s at 4.5 ms−1 in a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...