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Citations for Qiagen :
151 - 200 of 10000+ citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... we used the same set of two siRNAs (Qiagen) against HNF1B compared with negative and positive control siRNA (Qiagen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with two sterile 3 mm beads (Qiagen, Hilden, Germany) and a bead device at 20 Hz for 3 min ...
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bioRxiv - Microbiology 2019Quote: ... Two separate rounds of DNase treatment were performed (Qiagen RNase-free DNase ...
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bioRxiv - Genetics 2019Quote: ... Repaired DNA was purified on two MinElute columns (Qiagen) and eluted in 16 μl (32 μl final for each library) ...
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bioRxiv - Microbiology 2019Quote: ... Cultures were treated with two volumes of RNAprotect (Qiagen) incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Repaired DNA was purified on two MinElute columns (Qiagen) and eluted in 16 μl (32 μl final for each library) ...
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bioRxiv - Microbiology 2023Quote: ... immediately mixed with two volumes of RNAProtect Bacteria (Qiagen) and pelleted according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Two antisense LNA GapmeRs specifically targeting two different regions of LINC00607 (NR_037195.1) were designed and purchased from QIAGEN (see table below for sequences). LNAs were separately transfected into HUVECs with Lipofectamine RNAiMAX following the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 0.25 g of soil from all segments from two of the five 13C soil cores using the DNeasy PowerSoil DNA Extraction kit (Qiagen, Hilden, Germany) to determine the segment with the highest abundance of methanogens or methanotrophs based on the respective functional marker genes using qPCR as described below ...
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bioRxiv - Developmental Biology 2022Quote: ... was isolated from flash-frozen fetal liver (left lobe) and placenta tissues (two samples/placenta from separate cotyledons) using miRNeasy mini kit (Qiagen, Germantown, MD) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two PowerBead preparations of 0.5 g were used along with the PowerSoil Pro DNA Isolation kit (MoBio Labs/QIAGEN, Carlsbad, CA, USA) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... from each RNA sample was synthesized in two separate technical replicates from 150 ng of total RNA using QuantiTect Reverse Transcription Kit (Qiagen, cat.: 205311), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... unexpanded leaves was extracted from 14 individuals (7 early-blooming, 5 late-blooming, and the two parents) with a DNeasy Mini Plant Kit (Qiagen, Hilden, Germany) and quantified with a Quant-iTTM PicoGreenTM dsDNA assay kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... Two volumes of RNAprotect Bacteria Reagent (Qiagen, Valencia, CA, USA) were added to all samples and incubated at room temperature for 5min ...
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bioRxiv - Microbiology 2023Quote: ... with two 5-minute cycles on a TissueLyser II (Qiagen) separated by a 5-minute incubation on ice ...
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bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from young leaflets of each RIL and the two parents using DNeasy® Plant Mini Kit (Qiagen; Hilden, Germany). DNA quantification was performed with Qubit (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR reactions for each primer set were performed on RNA (50-75 ng/ul) from at least two biological replicates of the mutant allele and control (QIAGEN OneStep RT-PCR Kit, QIAGEN Inc., Cat # 210212). The bounds of where transcriptional termination and initiation occurs within Mu for each mutant allele was determined by presence of amplification from gDNA and absence of amplification from cDNA ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen). Then ...
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bioRxiv - Cancer Biology 2019Quote: ... Two pathway-directed RT2 Profiler PCR Arrays were selected from Qiagen: Mouse Extracellular Matrix & Adhesion Molecules (PAMM-013ZE-4 ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was loaded on two 1-ml Ni-NTA (Qiagen 30210) columns ...
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bioRxiv - Immunology 2022Quote: ... The tissue homogenization was performed using two Tungsten Carbide Beads (Qiagen) (3 mm ...
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bioRxiv - Microbiology 2022Quote: ... Cell cultures were then combined with two volumes of RNAprotect (Qiagen) in an anaerobic chamber ...
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bioRxiv - Microbiology 2023Quote: ... two swabs pre-moistened in nuclease-free water (Qiagen, Hilden, Germany) were inserted 2 cm into one nostril and rotated against the anterior nasal mucosa for up to 10 seconds ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were stopped by addition of two volumes of RNAprotect (Qiagen) then centrifuged at 5,000 g for 5 min ...
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bioRxiv - Neuroscience 2019Quote: ... on BioRad CFX96 real-time system using QuantiTect SYBR Green PCR Kit (Qiagen) along with gene specific forward (fwd ...
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bioRxiv - Molecular Biology 2021Quote: ... two frozen sections were directly collected into 750 µL RLT buffer (Qiagen) containing 40 mM dithiothreitol (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... two diaphragms of P2.5 C57Bl6 pups were collected in RNA later (Qiagen) before they were processed in RLT buffer containing 1% mercaptoethanol (Sigma ...
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bioRxiv - Genomics 2019Quote: ... total RNA was extracted from two independent liver samples using Qiazol (Qiagen) and DNase treated with DNA-free DNA Removal Kit (Ambion) ...
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bioRxiv - Biophysics 2022Quote: ... and proteins were purified by two successive nickel affinity chromatography (Qiagen®) steps ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: Cell samples from two independent differentiations were collected in Buffer RLT (QIAGEN) at the the time points described above (Figure 1A) ...
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bioRxiv - Genomics 2022Quote: ... exRNA was isolated from cell culture media by two methods: exoRNeasy (Qiagen) and Exosome Total Isolation Chip (ExoTIC) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from two-dimensional cultured cells using QIAshredder (QIAGEN) and PureLink RNA mini kit (InvitrogenTM ...
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bioRxiv - Cell Biology 2019Quote: ... Two hundred microliters of 1-mg/ml RNase A (Qiagen, Germantown, MD) was added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with two 5 mm stainless beads using a TissueLyser (Qiagen, CA, USA) at 50 Hz for 4 min ...
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bioRxiv - Microbiology 2022Quote: ... Samples were immediately exposed to two volumes of RNA protect reagent (Qiagen) and used for subsequent RNA isolation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... followed by two purification steps with Genomic tip 500/G (QIAGEN, Germany). To generate a high-quality genome assembly ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was incubated for two hours with Ni-NTA beads (Qiagen). Non-specifically bound proteins were removed by washing the beads with 20 mM NaPO4 pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... For two-way qPCR (analysis of MAPK11; Hs_MAPK11_1_SG QuantiTect Primer Assay, Qiagen); cDNA was first synthesised with High Capacity cDNA Reverse Transcription Kit (Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Amplified cDNA was prepared from total RNA using the Ovation PicoSL WTA System V2 Kit (NuGEN) and purified with the MinElute Reaction Cleanup Kit (Qiagen). RNA quality and quantity were assessed using a 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... The clinical Samples (cervical smear) for the HPV DNA test were processed using HPV Test Hybrid Capture® 2 protocol (QIAGEN). Samples with relative light units (RLU ...
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bioRxiv - Evolutionary Biology 2020Quote: ... aeruginosa isolates on a QIAcube system using a DNeasy Blood and Tissue kit (Qiagen) and sequenced on an Illumina HiSeq 2000 platform ...
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bioRxiv - Genetics 2019Quote: ... and total RNA was extracted using the RNeasy Protect Animal Blood System kit (Qiagen) according to the manufacturer’s protocol followed by concentration of RNA using NucleoSpin RNA clean-up XS kit (Macherey-Nagel) ...
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bioRxiv - Neuroscience 2021Quote: ... using SYBR-Green based detection system with QuantiNova SYBR green PCR Kit (Qiagen, 208052). Relative expression of mRNA was calculated through ΔΔCt method using the ribosomal protein RP49 RNA expression as internal control for each sample ...
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bioRxiv - Molecular Biology 2022Quote: Two independent siRNAs targeting YBX1 (Hs_YBX1-1 and Hs_YBX1-6 FlexiTube siRNA, Qiagen) and a non-targeting negative control siRNA (AllStars ...
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bioRxiv - Physiology 2022Quote: ... Two rounds of tissue homogenization were performed on the TissueLyserII machine (Qiagen, #85300) at 25 Hz ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 × 108 cells were mixed with two volumes of RNAprotect Bacteria Reagent (Qiagen). Samples were lysed in Tris-EDTA (pH 8.0 ...
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bioRxiv - Plant Biology 2023Quote: ... and ground for two minute at 20 Hz in a TissueLyser II (QIAGEN). Then ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was filtered and loaded on two 0.5 ml Ni-NTA (Qiagen) columns (one column/L growth ...
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bioRxiv - Cancer Biology 2024Quote: ... Two ml of a 50% slurry of washed Ni-NTA beads (Qiagen # 30210) was added to the supernatant of the 2-liter culture ...